FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440790
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAATAGAAGGTAGAAAGTCAATTAGAGACGATTACAAACTAACTGAC21840.15158350627086528No Hit
ATATTAAGGATCATCCTGTCTATTTCTGCTAATACCATCCCCTTAGCAGT17310.12014242186578197No Hit
TTCCTATACAGATGGCTCTAATTTGGGAAGTACACTGACTTTTTGACTTG17200.11937895182504042No Hit
GTATAGGAAATCTCCATTTTACAGGAGCGTAGTGCATCTGTTGTTTGTTT16130.11195247051964548No Hit
ATGTAGTACAAAGCTGACAGTGAACACTTTTACAAAATCACTGAAAAGAT15740.10924562219338002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)