FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837278
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAA500505.9777039406266494No Hit
ATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAA388864.644335573131027No Hit
CTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTT223742.6722307286229903No Hit
GAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCAT218772.612871710471313No Hit
ACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGT189082.2582702519354387No Hit
AAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGA160871.921345120736482No Hit
GTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCA148331.7715740769493524No Hit
GGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAA123031.4694044272033902No Hit
AGCGAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGG118161.4112397554933964No Hit
GAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGG110831.3236941613179853No Hit
TCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCA107891.2885803759324859No Hit
GTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGT101381.2108284225788806No Hit
ACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATC100121.1957796574136668No Hit
CCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAG96941.1577994405681267No Hit
GTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTAT94061.1234022630476377No Hit
CAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTT82970.9909492426649213No Hit
ATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAATG76930.9188107175872292No Hit
ATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGA76210.9102114232071069No Hit
GACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCAT70710.8445223689145063No Hit
GCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCT69770.8332955123626801No Hit
CTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCA69340.8281598226634403No Hit
ATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTA65740.7851633507628291No Hit
AGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGT65160.7782361414010639No Hit
CTCACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGG64780.773697624922666No Hit
AAACAAAGCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTT63940.7636651148125234No Hit
TAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAA63580.7593654676224624No Hit
GCCCTAAACAAAGCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTT63330.7563796015182531No Hit
ACATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAATGGCTTTTAAAGTTA58940.7039477927283411No Hit
GGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGG58580.6996481455382799No Hit
ACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCT57500.6867492039680966No Hit
AAGCAGGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTC56750.6777916056554693No Hit
GGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCC56740.6776721710113008No Hit
TGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGC56430.6739696970420815No Hit
GGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGG53790.6424389509816333No Hit
GCTTCTCACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACAT52710.6295400094114499No Hit
CTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGG51740.6179548489271186No Hit
GTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGAC51100.6103110317003433No Hit
CTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGACGATTTCTATGTTGG50600.604339299491925No Hit
AAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTT50390.6018311719643893No Hit
GCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTTTGCCAGG49200.5876184493083539No Hit
CTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAG46870.559790177217125No Hit
GGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGA46740.5582375268429363No Hit
CCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTT46310.5531018371436965No Hit
TCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTCTCCTTATC45430.5425915884568805No Hit
GTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAG45370.5418749805918703No Hit
CCCTAAACAAAGCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTTT44300.5290954736658553No Hit
ACCCAGGCGCCCTAAACAAAGCATTATTTTAGGGACCAACATAGAAATCG44200.5279011272241716No Hit
CATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAATGGCTTTTAAAGTTAC44050.5261096075616462No Hit
GGGAAATAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTG43800.5231237414574371No Hit
ACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAG41920.5006700283537845No Hit
TCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGC41800.4992368126237641No Hit
CACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAA39500.4717668444650403No Hit
GAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCC39180.4679449358516526No Hit
AGCGAAAGCAGGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAA36840.4399972291162553No Hit
CCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCT36210.43247284653364826No Hit
ATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTG35790.427456591478577No Hit
GGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTCTCCTT35470.4236346828651893No Hit
GCGAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGC34840.4161103002825824No Hit
TTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATA34160.4079887444791336No Hit
GTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGACGATTTC33980.405838920884103No Hit
ACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAA33940.40536118230742957No Hit
TGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAG33870.40452513979825105No Hit
GGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAG33200.3965230186389706No Hit
GCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAA32870.39258167538141453No Hit
GTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACTTTAAA31990.3820714266945985No Hit
GGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCA31660.3781300834370424No Hit
AAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATG31320.37406930553531803No Hit
GCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTA31190.3725166551611293No Hit
TTATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAA31190.3725166551611293No Hit
GCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTG30400.36308131827182843No Hit
ATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGAC29190.3486297263274563No Hit
ATTTCTATGTTGGTCCCTAAAATAATGCTTTGTTTAGGGCGCCTGGGTGG28680.3425385594748698No Hit
CAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAG27500.32844527146300273No Hit
CTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAGGG26970.32211523532207936No Hit
GCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAGG26830.32044315030372233No Hit
TTTCTATGTTGGTCCCTAAAATAATGCTTTGTTTAGGGCGCCTGGGTGGC26560.3172184149111764No Hit
AACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCC25860.30885798981939094No Hit
GATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGA25110.29990039150676356No Hit
GATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTT25000.2985866104209116No Hit
GCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATG24550.2932120514333352No Hit
GACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAGGGACCAACA24400.2914205317708097No Hit
CACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCT23930.2858071034948965No Hit
GACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCC23470.2803131098631518No Hit
GTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAA23260.2778049823356161No Hit
GGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTA22980.2744608122989019No Hit
ATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTCTCCTTAT22660.27063890368551424No Hit
CCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGG22540.2692056879554939No Hit
GCGAAAGCAGGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAAC22540.2692056879554939No Hit
GAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAG22470.2683696454463153No Hit
GTTTAGGGCGCCTGGGTGGCTCAGTCAGCTAAGCATCTGCCTTTAGTGCC22440.26801134151381023No Hit
CTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCC21720.259412047133688No Hit
GGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGA21090.25188766455108097No Hit
ATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTT21050.25140992597440753No Hit
AGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGC20730.24758801736101987No Hit
GGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAAT20560.24555762841015766No Hit
TATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGG20280.24221345837344346No Hit
CCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCAC19680.2350473797233416No Hit
CTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATT19050.22752299714073462No Hit
CCCTAAAATAATGCTTTGTTTAGGGCGCCTGGGTGGCTCAGTCAGCTAAG18730.22370108852734694No Hit
AAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAG18440.22023748384646435No Hit
GAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAGGGACCAACATAGA17880.21354914377303597No Hit
GGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAA17760.21211592804301557No Hit
ACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCA17440.20829401942962794No Hit
ATAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAA17060.20375550295123004No Hit
GGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAAC16500.19706716287780165No Hit
GTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGA16420.19611168572445473No Hit
GCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACA16310.1947979046386027No Hit
CCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTG16190.19336468890858236No Hit
CTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCAT15930.19025938816020485No Hit
CAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGC15630.1866763488351539No Hit
ACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGT15290.1826155709334295No Hit
AAATAATGCTTTGTTTAGGGCGCCTGGGTGGCTCAGTCAGCTAAGCATCT15270.1823767016450928No Hit
TAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACT15250.18213783235675607No Hit
TGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACT15250.18213783235675607No Hit
CATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTT14810.17688270801334802No Hit
CCCAGGCGCCCTAAACAAAGCATTATTTTAGGGACCAACATAGAAATCGT14100.16840284827739413No Hit
GAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTG13890.16589472074985848No Hit
GAAATAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGC13750.16422263573150137No Hit
ACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCC13370.1596841192531035No Hit
GCCATTGCCTGGCAAAAGAAAAGCAGACGATTTCTATGTTGGTCCCTAAA13240.15813146887891477No Hit
GAGCGAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTG13220.15789259959057805No Hit
TACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGAT13070.15610107992805258No Hit
TAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGT13000.155265037418874No Hit
TCTCACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGG12910.15419012562135873No Hit
GGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTA12820.15311521382384347No Hit
GCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTT12560.15000991307546596No Hit
ATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACTTTA12280.14666574303875177No Hit
CTTTTAAAGTTACTGACAATGATCATTCCCACTATTTCCCAAAAGAAAGA12260.14642687375041502No Hit
ATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATG12210.1458297005295732No Hit
TGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAG11920.14236609584869064No Hit
GCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGG11810.1410523147628386No Hit
CGAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCT11330.13531945184275712No Hit
AGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATT10890.1300643274993491No Hit
AAGCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTTTGCCA10400.12421202993509921No Hit
CAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTAC10250.12242051027257375No Hit
AGCAGGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTCT10090.12050955596587991No Hit
CCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCA9740.11632934341998716No Hit
CAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCC9550.11406008518078821No Hit
CTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAA9350.11167139229742093No Hit
TCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACTTTAAAAG9280.11083534978824237No Hit
CCAACATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAATGGCTTTTAAAG9220.11011874192323218No Hit
TTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTC8820.10534135615649759No Hit
GGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTG8580.10247492469645685No Hit
GGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAA8410.10044453574559464No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)