FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953125
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATACAGGTGCCCCTGATAAGGTCTTTATAGATTTTGGATACTCGCCC92290.47252479999999997No Hit
CCCTTTATCTGATAATGTTATTCGCAAATATCTTCTCCCATTCTCTCCAT84390.4320768No Hit
TCCTTACACCACCTTACACACAAAAACAGACTCAAAATGGATGAAAGACC70390.3603968No Hit
TTCTAGTACTGAACAAATCTTGACTTTCTTTGGAAAAGCTGAACAAAAGA66650.341248No Hit
CTTCAATAAATGGTGTTGGAAAAACTGGACAGCCACATGCAGAAGAATGG63660.3259392No Hit
GTCTTTATAGATTTTGGATACTCGCCCTTTATCTGATAATGTTATTCGCA62790.3214848No Hit
ACCTTACACACAAAAACAGACTCAAAATGGATGAAAGACCTAAATGTGAG62510.3200512No Hit
TCATAGGACCCTGGGATCATGACCTTAGCAGAAGGCAGATGCTTAATGAC42620.2182144No Hit
AAGTCATACAGGTGCCCCTGATAAGGTCTTTATAGATTTTGGATACTCGC42170.21591039999999997No Hit
CTACAGGGTCATTTGGTTGAGGCCTTTCTGCCACATTACCTAAACCAACC41040.2101248No Hit
CTTAATGACTAAGTCATACAGGTGCCCCTGATAAGGTCTTTATAGATTTT40560.2076672No Hit
ATGCAGAAGAATGGACTATTTCCTTACACCACCTTACACACAAAAACAGA39180.2006016No Hit
CTCAATGACTGAGCCACCCTGGCACCCCGGCAATTACTTTTAACTCCAGG39130.20034559999999998No Hit
CACAAAAACAGACTCAAAATGGATGAAAGACCTAAATGTGAGATAGGAAT38620.19773439999999998No Hit
CTATACGCACAGTCTCAGCTCTCCTGGCTGGAAAAAGCTCAGAGCTCTCG38410.1966592No Hit
GATAAGGTCTTTATAGATTTTGGATACTCGCCCTTTATCTGATAATGTTA37170.19031040000000002No Hit
GTGTGTAGCAGCTCGAGAGGCACCGAGCAGCTGAGCCACGCGAAAACCTG37120.1900544No Hit
ACCTAAATGTGAGATAGGAATCCATCAAAATCCTAGAGAACACACACAGC36460.18667519999999999No Hit
GTCCTAGTGAGAGCGGCGCTCAGAAAGAGTCCGACAAGAGATCAAATGAG35400.181248No Hit
CACTAGGACTCTGGCCCTGACTTTTCTCTCTGCCCATGATAGCCAAGCGA34380.1760256No Hit
ACCCTGTAGAGCGTAATTACCTATTAAAGGGATGGGAGGCACCAAATGGA33410.1710592No Hit
GCCACATGCAGAAGAATGGACTATTTCCTTACACCACCTTACACACAAAA33390.17095680000000002No Hit
CTCTTCAATAAATGGTGTTGGAAAAACTGGACAGCCACATGCAGAAGAAT33150.169728No Hit
GAAAAAAGACAGTCTCTTCAATAAATGGTGTTGGAAAAACTGGACAGCCA31490.1612288No Hit
ACCTAAACCAACCCGTCAGAGGACCCTTACTTAATCTTCAGACACCCTGC30910.15825920000000002No Hit
CAATAAATGGTGTTGGAAAAACTGGACAGCCACATGCAGAAGAATGGACT30690.15713280000000002No Hit
CCTTAGCAGAAGGCAGATGCTTAATGACTAAGTCATACAGGTGCCCCTGA29900.153088No Hit
GTCTTTGGTGTTGTTGAGTGTTTCTTTTGCTGTGCAAAAGGAAGTCCCAG28550.146176No Hit
GGAAAAAAGACAGTCTCTTCAATAAATGGTGTTGGAAAAACTGGACAGCC28250.14464No Hit
GATAATGTTATTCGCAAATATCTTCTCCCATTCTCTCCATTGTCTTTGGT27080.1386496No Hit
GTGTCAGGCGTTGTGCTAAATACTTTCCACGTAGGGAGGATACGCTGCCT26880.1376256No Hit
GACTATTTCCTTACACCACCTTACACACAAAAACAGACTCAAAATGGATG26320.1347584No Hit
AGAATGGACTATTTCCTTACACCACCTTACACACAAAAACAGACTCAAAA26300.134656No Hit
TTCTTTTGCTGTGCAAAAGGAAGTCCCAGTAGTTCATTTTGCCCTTGCTT25960.13291519999999998No Hit
ATTAAAGGGATGGGAGGCACCAAATGGAGCAGATAAGAAAACCAGAAACA25100.12851200000000002No Hit
CCTTAAAGCCTGGAATACATCCGTCCCACCTAGAAACGGTAAGGAAGTCG25070.12835839999999998No Hit
GGTCTTTATAGATTTTGGATACTCGCCCTTTATCTGATAATGTTATTCGC24580.1258496No Hit
CTCCCATTCTCTCCATTGTCTTTGGTGTTGTTGAGTGTTTCTTTTGCTGT24500.12544No Hit
GTATTTATGCTGTAAGATGTCTGGACTGGAAAGTACTAATCGGAGGGAAC24090.1233408No Hit
GTGTTGGAAAAACTGGACAGCCACATGCAGAAGAATGGACTATTTCCTTA23410.1198592No Hit
CCTTACACCACCTTACACACAAAAACAGACTCAAAATGGATGAAAGACCT22890.1171968No Hit
ATCCTAGAGAACACACACAGCAACCTCTTTGAACTTCTTCCTAGACACAT22710.1162752No Hit
CTCTTAGCTTATGGAAGCCAAGTCTTCCTGGATCATGCCAGAAGTTCCTG22420.11479039999999999No Hit
GTCCAAAGGAAAAAAGACAGTCTCTTCAATAAATGGTGTTGGAAAAACTG22120.1132544No Hit
GTCTAGGAAGAAGTTCAAAGAGGTTGCTGTGTGTGTTCTCTAGGATTTTG21790.11156479999999999No Hit
ATTTCCTTACACCACCTTACACACAAAAACAGACTCAAAATGGATGAAAG21780.1115136No Hit
GCTAAATACTTTCCACGTAGGGAGGATACGCTGCCTGCTGCCTTCTCAGA21490.11002880000000001No Hit
GGGAAGGACTGTGTGTAGCAGCTCGAGAGGCACCGAGCAGCTGAGCCACG21430.1097216No Hit
CTCCCAATCATCTCCCAATTTCTAGTACTGAACAAATCTTGACTTTCTTT21270.10890240000000001No Hit
TTCCTAGACACATCATCAAAAGCAAGGGAAGCAAGGGCAAAATGAACTAC21060.10782719999999998No Hit
TGCCTGCAGTGTGTCAGGCGTTGTGCTAAATACTTTCCACGTAGGGAGGA20970.1073664No Hit
CCCTGGCACCCCGGCAATTACTTTTAACTCCAGGGTTTTTTGTTGTTGTT20910.1070592No Hit
ATTTAAGGTCAAAGGGGCAACTCAAGGTCAAGTATCTGAATAACTAGCAG20900.10700799999999999No Hit
GGCAGAGAGAGGGGGGTAGGCAGGCTCCCTGCCAAGCAGAGAGCGGGGAC20870.1068544No Hit
GGTTTAGGTAATGTGGCAGAAAGGCCTCAACCAAATGACCCTGTAGAGCG20340.10414079999999999No Hit
GGAAAGAATGTCCAAAGGAAAAAAGACAGTCTCTTCAATAAATGGTGTTG20210.10347519999999999No Hit
AAATAATAAAGAGCTTTTCAAATCAAAAACACTGTAGCATAAATCCTGAA19750.10112No Hit
GTACAGTGATCCAGGTCAGGGCCCTCGGCGAGGATGCTGGGGACAAGCCA19720.1009664No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)