FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848907
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG80500.4353923696540713No Hit
GTATAGAATTACCCATTGTCAAACAATGTATCAGACAACACAGGGTCTTG68310.36946152510645475No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT54310.2937411129927033No Hit
GTGTGTGTGTTAAGCATGGAACACCACAAACCAGGTATTTTTTGCATGGT36180.19568317930539503No Hit
ATCCTAAAGGTGCCAAATTTATCTCCAGATAAATTTGGAAGCAACTGTGG35810.19368199698524588No Hit
ATATTACACTTGGGAAAAGGAAAAAAAGTCACCAGCAGTTGAACTATTCA33790.18275662323740458No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC32280.17458963593084997No Hit
GTCTTGGCACTGTGTGTGTGTTAAGCATGGAACACCACAAACCAGGTATT31140.16842383094444446No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG28550.15441555470340043No Hit
GTATAAATATTACACTTGGGAAAAGGAAAAAAAGTCACCAGCAGTTGAAC28320.1531715765043888No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA27800.15035910405444947No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT27750.15008867401118609No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA27690.14976415795926998No Hit
TCCTAAAGGTGCCAAATTTATCTCCAGATAAATTTGGAAGCAACTGTGGA27030.14619448138819313No Hit
CTCTAGGAATTCATAAAAGCATAAGAGGCAGCAAAATACAGAGGCAGATT25850.13981233236717694No Hit
GTTCTATGCTGCTCTTCCATCAGTAAGGCATGAGATCAGCATCAGTAGCC24340.1316453450606223No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA23790.12867061458472492No Hit
CCCATGGATTATCCTAAAGGTGCCAAATTTATCTCCAGATAAATTTGGAA21720.11747481079362022No Hit
GAATTATGAAGACTATTTGGGAGCTACCTCCTGTTGTCCAGGATGAGACA21530.11644717662921932No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA21450.11601448855999788No Hit
CTTTAGTTCTATGCTGCTCTTCCATCAGTAAGGCATGAGATCAGCATCAG20920.11314793010140586No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA19760.10687395309769501No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA19630.10617083498521018No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG19590.10595449095059946No Hit
GTATAGCCCAGTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGC19490.10541363086407267No Hit
GGATAATGGATCCTGCTACTTGGCATGCAAAGAAGGCAATAGGAAACGTT19310.10444008270832443No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA19190.10379105060449227No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT18990.10270933043143868No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)