FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638159
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGAGATGGTTGATAATAGGACTTCATGACTCTATGTCTGCAAAG32270.1969894253244038No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT26350.16085129709631363No Hit
TTATAGCACATGTCCATGTAAGCTTAGACATAAAACTCAAGAGGTTTTTA25240.1540753980535467No Hit
AAATAACACTGTACTCCTGGACCTAAAGAAGACAATGAAAAAAGGTATTT24550.14986335270263754No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG21160.12916939076121425No Hit
GGGTAAGGGTGTGAGGCCTGGGCTCTGGGCCAATTCTATCACCACTTAGT19680.12013485870419172No Hit
ATACAGCGCTGAATACCTTTTCAGTTTTTCCCCAAAATAACACTGTACTC18040.1101236204788424No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC16530.10090595601525859No Hit
GTGATGGAGGGACCCAGACAAGATGACCGGAGCCCCACCCAGCCTTGCTG16530.10090595601525859No Hit
CCCTATATCCAGGGTAAGAGAAATTAAAGTACTACTGAGAAATCACTTTA16440.1003565587955748No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)