FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_A11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_A11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657396
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACAGGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATT126310.7620991000340293No Hit
GCCTTATAAACCATGTTAAGAATCCAGACTTTATCCTATTAAAGGTGACA46110.27820750140581973No Hit
TAGTATTACAGGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACA44150.2663817216887213No Hit
GTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATTCCTTTTTGT36960.22300041752242677No Hit
ATATAGTATTACAGGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAA30590.18456663344185698No Hit
GATTATACTTGTGCTATGAAGGAAATTAAGTGGAGAGAGCTGAAACTGGG25690.15500218414911102No Hit
GTGATAAGGTGTAGGAAATTCAAGTCACGTGTTAAACGGAATGATTCTAG24060.14516747958846285No Hit
TATTAAAGGTGACAGGACTCAGGGAAAGGGAATGTTTTTGAAAGATTATA23650.14269371954560045No Hit
ATTAAAGGTGACAGGACTCAGGGAAAGGGAATGTTTTTGAAAGATTATAC22850.13786687068147865No Hit
CTTATAAACCATGTTAAGAATCCAGACTTTATCCTATTAAAGGTGACAGG21850.13183330960132641No Hit
GTGTTAATATGTTGAGGGGGAGAGAAATACAACAGACAGTGATAAGGTGT20830.12567907729957112No Hit
GTGAAAAGCAAACTGCCTTATAAACCATGTTAAGAATCCAGACTTTATCC19970.12049021477064022No Hit
AGATAGTGCTCTGAGGCAAGCAGGAGACAGACTGTGAAAAGCAAACTGCC19900.12006786549502955No Hit
CAGGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATTCCTTTT19380.11693041373335039No Hit
GGTATAGTTCTTCTTTTTTTCTGCTTTGTCTCTGCAAACATTCCTTTTTG18660.11258624975564079No Hit
GTCCTAGATAAAGGTTATTTATGGGGAAGAGCATAAAGAAAGGGAATGAT18240.11005215410197684No Hit
CTATTAAAGGTGACAGGACTCAGGGAAAGGGAATGTTTTTGAAAGATTAT17450.10528564084865658No Hit
GTTAAAAACAGGCACATGATGGAAATAAACCATTGAGACAAGATCCAAAA16940.10220852469777891No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)