FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S7_R1_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S7_R1_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663752
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG44960.2702325827406969No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA39260.23597266900355343No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC35790.21511619520216954No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT34820.20928599935567319No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA29520.17743029009131167No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT29460.17706965942039438No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA29430.17688934408493573No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT28320.1702176766729657No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT25280.15194572267982248No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC22590.1357774476003635No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC22110.13289240223302512No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA21900.13163019488481456No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG21550.1295265159711303No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC21220.12754304728108515No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT20780.12489842236102495No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT18290.109932249517957No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC17740.10662646836788175No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT17640.10602541724968625No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG17290.103921738336002No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT17090.10271963609961099No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT16700.10037553673864855No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC16680.10025532651500946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)