FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S7_R1_G12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S7_R1_G12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11230
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCT5274.692787177203918No Hit
AGCGAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGT5024.470169189670525No Hit
CTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAA3813.3926981300089047No Hit
AAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAG3152.8049866429207477No Hit
GCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTG3012.6803205699020483No Hit
AAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCA2332.07479964381122No Hit
GCGAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTG1741.5494211932324131No Hit
AGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAG1661.4781834372217275No Hit
GCGAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTG1641.460373998219056No Hit
TGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTT1541.3713268032056989No Hit
GATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGA1311.1665182546749777No Hit
GGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCG1181.0507569011576134No Hit
AAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGGTT1171.0418521816562778No Hit
GCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGC1131.006233303650935No Hit
CTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCA1090.9706144256455922No Hit
CCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCGC1090.9706144256455922No Hit
ACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGA1030.917186108637578No Hit
CGAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGC970.8637577916295637No Hit
GACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCT960.8548530721282279No Hit
CACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGG940.8370436331255565No Hit
GCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTG870.7747105966162066No Hit
CCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCAC850.7569011576135352No Hit
CCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGC840.7479964381121995No Hit
GGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGT830.7390917186108638No Hit
TCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACC820.730186999109528No Hit
GCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGAC750.667853962600178No Hit
GAGCGAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCC630.5609973285841496No Hit
GTTGAAGGATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACT570.5075690115761353No Hit
GGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGC560.4986642920747996No Hit
GCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCG510.45414069456812106No Hit
GAGCGAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCG510.45414069456812106No Hit
GCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCC470.41852181656277826No Hit
AAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGC460.40961709706144256No Hit
GCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCT460.40961709706144256No Hit
GTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGTT450.4007123775601068No Hit
ACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGG450.4007123775601068No Hit
AAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGTTTACT450.4007123775601068No Hit
TCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCG440.3918076580587711No Hit
GCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGC430.38290293855743546No Hit
GCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAG430.38290293855743546No Hit
GCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTC420.37399821905609976No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATAAGTCTGTCTCTTATACA410.365093499554764No Hit
TTATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGTTTACTATTTA410.365093499554764No Hit
GTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTATTGTATATC410.365093499554764No Hit
GTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAA410.365093499554764No Hit
GGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGC400.3561887800534283No Hit
CGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCG390.3472840605520926No Hit
GGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTG390.3472840605520926No Hit
CCTTCGTGCGCGATGCTTCGCACGAGCTGCGCACGCCGCTCACGGCCCTC380.3383793410507569No Hit
GGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCAC380.3383793410507569No Hit
GTTTACTATGTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTA380.3383793410507569No Hit
TTTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGCAC370.3294746215494212No Hit
ACTTATTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTCTTATACA360.32056990204808544No Hit
CGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCT360.32056990204808544No Hit
GTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGA360.32056990204808544No Hit
CTTTTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGC360.32056990204808544No Hit
TTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGCACG350.3116651825467498No Hit
CTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTT340.3027604630454141No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAAAATTAAAA330.2938557435440784No Hit
CTATACAACATAGTAAACTATACAACAACACTTTTATAATATTTACTACT320.28495102404274264No Hit
AGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAA320.28495102404274264No Hit
TTCGTGCGCGATGCTTCGCACGAGCTGCGCACGCCGCTCACGGCCCTCAT310.27604630454140694No Hit
TTATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGCTGTCTCTTAT300.26714158504007124No Hit
TGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAA300.26714158504007124No Hit
GTAGTAAATATTATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGT290.25823686553873554No Hit
CACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCG290.25823686553873554No Hit
GCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGCACGCCGCTCACGG290.25823686553873554No Hit
CGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGC270.2404274265360641No Hit
CTATACAACATAGTAAACTATACAACAACACTTTTATAACTGTCTCTTAT270.2404274265360641No Hit
AGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGT270.2404274265360641No Hit
CGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCC270.2404274265360641No Hit
CAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACC260.2315227070347284No Hit
CAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGG260.2315227070347284No Hit
CTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCG260.2315227070347284No Hit
GAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCT250.22261798753339268No Hit
CAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAAAATTAAAAATTAA240.21371326803205698No Hit
AGGATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACA240.21371326803205698No Hit
GCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACA230.20480854853072128No Hit
ATTGTATATCCTTCAACACTTCAGTTGATACCTGCTTTCGCTCTGTCTCT230.20480854853072128No Hit
CTATACAACATAGTAAACTATACAACAACACTTTTATCTGTCTCTTATAC220.19590382902938555No Hit
GCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCGCG220.19590382902938555No Hit
GTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGTTTACTATGTTGT210.18699910952804988No Hit
CAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCAT210.18699910952804988No Hit
GCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGC210.18699910952804988No Hit
GCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGG210.18699910952804988No Hit
AGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTCTGT200.17809439002671415No Hit
ATTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTCTTATACACATC200.17809439002671415No Hit
AAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCG200.17809439002671415No Hit
ATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACAACA200.17809439002671415No Hit
GGCCAGCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGCACGCCGCT200.17809439002671415No Hit
GTTGTATAGTTTACTATGTTGTATAGTTTACTATTTAATTTTTAATTTTT200.17809439002671415No Hit
GAGCAAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCG190.16918967052537845No Hit
GAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGTTTAC190.16918967052537845No Hit
ATGCTTCGCACGAGCTGCGCACGCCGCTCACGGCCCTCATCGGCGAGCTG190.16918967052537845No Hit
GCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGG180.16028495102404272No Hit
GGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGGTTGAAGCGCTGG180.16028495102404272No Hit
TTTTTCTACTTATTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTC180.16028495102404272No Hit
ATGTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTATTGTATA180.16028495102404272No Hit
ACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCGCGATGCT180.16028495102404272No Hit
TTGTATATCCTTCAACACTTCAGTTGATACCTGCTTTCGCTCTGTCTCTT170.15138023152270705No Hit
TGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGG170.15138023152270705No Hit
TGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAG170.15138023152270705No Hit
GTGTTGAAGGATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAA170.15138023152270705No Hit
TTTCTACTTATTGTATATCCTTCAACACTTCAGTTGCTGTCTCTTATACA170.15138023152270705No Hit
GATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACAAC170.15138023152270705No Hit
GCCTCGAGGCGGTCGAGCAGGCGGTTGAAGCGCTGGGTGAGCAGGCCGAT170.15138023152270705No Hit
GAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGA170.15138023152270705No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAAACTGTCTC160.14247551202137132No Hit
CGAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGC160.14247551202137132No Hit
ATACAACATAGTAAACTATACAACAACACTTTTATAATATTTACTACTAA160.14247551202137132No Hit
GTATAGTTTACTATGTTGTATAGTTTACTATTTAATTTTTAATTTTTTCT160.14247551202137132No Hit
CGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGT160.14247551202137132No Hit
CGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGG150.13357079252003562No Hit
CGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCG150.13357079252003562No Hit
ACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACAACATAGT150.13357079252003562No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAACTGTCTCTT150.13357079252003562No Hit
TTTCTACTTATTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTCTT150.13357079252003562No Hit
AGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAAAATT150.13357079252003562No Hit
AGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACCTGTCTCTTAT150.13357079252003562No Hit
TTATAAAAGTGTTGTTGTATAGTTTACTATCTGTCTCTTATACACATCTG140.1246660730186999No Hit
ATACAACATAGTAAACTATACAACAACACTTTTATAACTGTCTCTTATAC140.1246660730186999No Hit
GCTTCGCACGAGCTGCGCACGCCGCTCACGGCCCTCATCGGCGAGCTGGA140.1246660730186999No Hit
CCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGC140.1246660730186999No Hit
ATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGCTGTCTCTTATAC140.1246660730186999No Hit
ACTTATTGTATATCCTTCAACACTTCAGTTGCTGTCTCTTATACACATCT140.1246660730186999No Hit
GGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGGTTGA140.1246660730186999No Hit
ATAGTAAACTATACAACAACACTTTTATAATATTTACTACTAAACCTTGT140.1246660730186999No Hit
GCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCG140.1246660730186999No Hit
CAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCT130.1157613535173642No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATCTGTCTCTTATACACATC130.1157613535173642No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAACTGTCTCTTATACACATCT130.1157613535173642No Hit
CCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGC130.1157613535173642No Hit
CCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGGTTGAAGCGCTGGGTG130.1157613535173642No Hit
GGATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACAA130.1157613535173642No Hit
TTTTTCTACTTATTGTATATCCTTCAACACTTCAGTTGATACCTGCTTTC130.1157613535173642No Hit
AGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGA130.1157613535173642No Hit
GCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAA130.1157613535173642No Hit
TTACTATGTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTATT130.1157613535173642No Hit
ATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAA130.1157613535173642No Hit
GCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAA130.1157613535173642No Hit
ACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTC130.1157613535173642No Hit
TTGTATATCCTTCAACACTTCAGTTGATACCTGCTCTGTCTCTTATACAC120.10685663401602849No Hit
GCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATCTGTCTCTT120.10685663401602849No Hit
GCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCA120.10685663401602849No Hit
AGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATCTGTCTCT120.10685663401602849No Hit
AAACTATACAACATAGTAAACTATACAACAACACTTTTATAATATTTACT120.10685663401602849No Hit
AGCAGGTATCAACTGAAGTGTTGAAGGATATACAATCTGTCTCTTATACA120.10685663401602849No Hit
TTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTCTTATACACATCT120.10685663401602849No Hit
CGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAA120.10685663401602849No Hit
CTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGT120.10685663401602849No Hit
GAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGA120.10685663401602849No Hit
GCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGGTTGAAGC120.10685663401602849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)