FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S7_R1_F01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S7_R1_F01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893765
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA52640.27796479499832344No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA46610.24612346304847751No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC39090.2064142066201456No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT34280.18101506786744925No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT33970.17937811713702598No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC29110.15371495407297103No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG28940.15281727141435184No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC28860.1523948325161781No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA28590.1509691012348417No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC26860.1418338600618345No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT26190.13829593428962939No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT25720.13581410576285863No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA25620.13528605714014147No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG25270.13343788696063133No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT24830.13111447302067575No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG24770.13079764384704543No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC24490.12931910770343735No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC24020.1268372791766666No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA23590.1245666700989827No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT23290.12298252423083118No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA23070.12182081726085338No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT22790.12034228111724528No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT22690.11981423249452809No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT22640.1195502081831695No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA22340.11796606231501795No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC22210.11727959910548563No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG21110.11147106425559666No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA20880.11025655242334713No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA20450.10798594334566326No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT20090.10608496830388144No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT19850.1048176516093602No Hit
AACTTATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA19850.1048176516093602No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT19700.10402557867528442No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT19690.10397277381301269No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT19330.10207179877123086No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA19150.10112131125033993No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG19070.1006988723521662No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT19070.1006988723521662No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT19010.10038204317853588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)