FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S7_R1_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S7_R1_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15207
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGC4883.209048464522917No Hit
AGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCC4032.6500953508252776No Hit
GGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAG3702.4330900243309004No Hit
GAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGA3392.229236535805879No Hit
CTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCC3202.1042940750969947No Hit
CCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATC3072.0188071282961797No Hit
GGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTG2951.939896100480042No Hit
AAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGAT2711.7820740448477674No Hit
GTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCC2611.7163148550009864No Hit
GTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGG2431.5979483132767802No Hit
GTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGT2301.512461366475965No Hit
ATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATT2151.4138225817057934No Hit
GCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAA2021.328335634904978No Hit
GATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATC1941.2757282830275531No Hit
AGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCG1881.2362727691194846No Hit
GCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTT1851.2165450121654502No Hit
AGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTT1821.1968172552114158No Hit
AGCGAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCG1731.1376339843493128No Hit
CCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTC1681.1047543894259222No Hit
CCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAATTCAACGGCATCG1661.091602551456566No Hit
CTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTT1641.0784507134872099No Hit
TCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCC1591.0455711185638195No Hit
GGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATG1591.0455711185638195No Hit
CTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGA1551.0192674426251067No Hit
AAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAG1500.9863878477017163No Hit
GTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTT1470.966660090747682No Hit
GTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGC1360.8943249819162228No Hit
CCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCC1320.8680213059775104No Hit
GGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAA1240.8154139541000854No Hit
GAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGG1210.7956861971460512No Hit
CGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGC1210.7956861971460512No Hit
GGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTG1140.7496547642533045No Hit
GGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGG1110.7299270072992701No Hit
AGCGAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAG1030.6773196554218452No Hit
CTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCA1000.6575918984678109No Hit
CCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCG1000.6575918984678109No Hit
CAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGA980.6444400604984546No Hit
GCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGA980.6444400604984546No Hit
CAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTG960.6312882225290984No Hit
CTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTC950.6247123035444203No Hit
GAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACC950.6247123035444203No Hit
AGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACT950.6247123035444203No Hit
GGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGG930.6115604655750642No Hit
CCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATG930.6115604655750642No Hit
CTGCATGGGTTGGCGGACGGGAGTGCGTGAGAGCGTGGTAACGAGTCGAT930.6115604655750642No Hit
TTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCG870.5721049516669955No Hit
CTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGA870.5721049516669955No Hit
GCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGC850.5589531136976392No Hit
TGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTG840.5523771947129611No Hit
CCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCAT830.545801275728283No Hit
AGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACG790.5194975997895707No Hit
CGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTG760.49976984283553627No Hit
TTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTC740.48661800486618007No Hit
AGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGG740.48661800486618007No Hit
GAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGA690.45373840994278947No Hit
CCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAAT690.45373840994278947No Hit
GAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTTGGCGGACG670.4405865719734333No Hit
ACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTC660.4340106529887552No Hit
GCGAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGT640.4208588150193989No Hit
CGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGC640.4208588150193989No Hit
AAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGA640.4208588150193989No Hit
CCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCA610.40113105806536464No Hit
GGGAAAGTGGATCGGCGGACGCTGCATGGGTTGGCGGACGGGAGTGCGTG600.39455513908068657No Hit
CTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCT590.3879792200960084No Hit
TCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCAT590.3879792200960084No Hit
CCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCC560.3682514631419741No Hit
GGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGG550.36167554415729597No Hit
AGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCA550.36167554415729597No Hit
TCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCC540.3550996251726179No Hit
CACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCC530.34852370618793976No Hit
AACCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAATTCAACGGCAT520.34194778720326163No Hit
TTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCAC510.33537186821858356No Hit
GTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGT490.3222200302492273No Hit
AGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATA480.3156441112645492No Hit
CCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAATTCAAC470.30906819227987115No Hit
AAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGT450.2959163543105149No Hit
CTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCAT450.2959163543105149No Hit
GAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTAC440.2893404353258368No Hit
GCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCAC400.26303675938712434No Hit
GGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGG390.2564608404024462No Hit
GAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGT390.2564608404024462No Hit
TCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACC390.2564608404024462No Hit
GCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACG390.2564608404024462No Hit
GGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGT380.24988492141776814No Hit
GCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCG380.24988492141776814No Hit
GCGTCCGCCGATCCACTTTCCCAGTCGAATTCAACGGCATCGCAGACACG350.2301571644637338No Hit
GTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTTGGCGG350.2301571644637338No Hit
ACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCG340.2235812454790557No Hit
GAGCGAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGC340.2235812454790557No Hit
GCGAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGC330.2170053264943776No Hit
ACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCG320.21042940750969946No Hit
ATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAATTCAACGGCATCGCAG320.21042940750969946No Hit
GTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAG310.20385348852502136No Hit
GCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGT310.20385348852502136No Hit
ACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCCA310.20385348852502136No Hit
GCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTT290.19070165055566515No Hit
TCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCC290.19070165055566515No Hit
CGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGG280.18412573157098705No Hit
CGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCAC280.18412573157098705No Hit
GCATGGGTTGGCGGACGGGAGTGCGTGAGAGCGTGGTAACGAGTCGATGG270.17754981258630895No Hit
GTCGAATTCAACGGCATCGCAGACACGAGCGTGATCCGCTCCGGTACCTT270.17754981258630895No Hit
GGTTGGCGGACGGGAGTGCGTGAGAGCGTGGTAACGAGTCGATGGGAGGA270.17754981258630895No Hit
ACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGAT270.17754981258630895No Hit
AGCAAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCG270.17754981258630895No Hit
GTGGATCGGCGGACGCTGCATGGGTTGGCGGACGGGAGTGCGTGAGAGCG270.17754981258630895No Hit
CCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCT260.17097389360163082No Hit
CTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCA260.17097389360163082No Hit
CGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGAT260.17097389360163082No Hit
CGAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTC250.1643979746169527No Hit
GGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTC250.1643979746169527No Hit
GCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTC250.1643979746169527No Hit
GCGTGAGAGCGTGGTAACGAGTCGATGGGAGGATGGAGGAAAGGGGAACG250.1643979746169527No Hit
CTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTT240.1578220556322746No Hit
CACTTTCCCAGTCGAATTCAACGGCATCGCAGACACGAGCGTGATCCGCT230.15124613664759648No Hit
CCGATCCACTTTCCCAGTCGAATTCAACGGCATCGCAGACACGAGCGTGA230.15124613664759648No Hit
GAGCGAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAA230.15124613664759648No Hit
ATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAAC230.15124613664759648No Hit
AGCGTGATCCGCTCCGGTACCTTGTATGCCGCCAGCCTATCCGCCACAAA230.15124613664759648No Hit
CGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCT220.1446702176629184No Hit
GGCGGACGCTGCATGGGTTGGCGGACGGGAGTGCGTGAGAGCGTGGTAAC220.1446702176629184No Hit
GCAAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGT210.13809429867824027No Hit
CCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTTGGC210.13809429867824027No Hit
TCGCAGACACGAGCGTGATCCGCTCCGGTACCTTGTATGCCGCCAGCCTA210.13809429867824027No Hit
ATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTT210.13809429867824027No Hit
CCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCC200.13151837969356217No Hit
CATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAAT200.13151837969356217No Hit
TCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGG200.13151837969356217No Hit
TCCCAGTCGAATTCAACGGCATCGCAGACACGAGCGTGATCCGCTCCGGT190.12494246070888407No Hit
AGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAG190.12494246070888407No Hit
CCACTTTCCCAGTCGAATTCAACGGCATCGCAGACACGAGCGTGATCCGC190.12494246070888407No Hit
ATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAG180.11836654172420596No Hit
GCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTTGG180.11836654172420596No Hit
GAGCAAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGC180.11836654172420596No Hit
GAGCAAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAA180.11836654172420596No Hit
GCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACT180.11836654172420596No Hit
ACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCC180.11836654172420596No Hit
GTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCC170.11179062273952785No Hit
AGCAAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAG170.11179062273952785No Hit
GGACGGGAGTGCGTGAGAGCGTGGTAACGAGTCGATGGGAGGATGGAGGA170.11179062273952785No Hit
CATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCA170.11179062273952785No Hit
CAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACT160.10521470375484973No Hit
GTGCGTGAGAGCGTGGTAACGAGTCGATGGGAGGATGGAGGAAAGGGGAA160.10521470375484973No Hit
CACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCT160.10521470375484973No Hit
TAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTA160.10521470375484973No Hit
GGAAAGTGGATCGGCGGACGCTGCATGGGTTGGCGGACGGGAGTGCGTGA160.10521470375484973No Hit
GGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTG160.10521470375484973No Hit
GGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGT160.10521470375484973No Hit
CCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATA160.10521470375484973No Hit
GATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGC160.10521470375484973No Hit
CTTGTATGCCGCCAGCCTATCCGCCACAAACTGACGCAACTCCTCTACCC160.10521470375484973No Hit
GTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGC160.10521470375484973No Hit
AAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAG160.10521470375484973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)