FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S7_R1_A07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S7_R1_A07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650212
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTATTATGGATGAGAAAAAGAACTCTTGCTAATATTTAATTCCTTTTC83630.5067833708638647No Hit
ATTTAATTCCTTTTCTCCTAAGAAAAGCTTATCTCTGAAAGGTTTTGCAA62520.3788604130863186No Hit
GTATTAGATTATCTGTTAAGTTCCCCCTGCAGATCTGGGCATTAGCCTCA52260.3166865832996003No Hit
AAATTGTATTGTGGGGTTACTGGTATATTGAACAACTCTTGTATTTGGAT48230.2922654786172928No Hit
GAAATAGATACCTTCCTACAGTAAATTGTATTGTGGGGTTACTGGTATAT38130.2310612212249093No Hit
CATTTAATGAGATGTAAAAATTATAATGGCATTTTTCTTTTAGTAGCCAG33950.20573114242291293No Hit
TGCTAATATTTAATTCCTTTTCTCCTAAGAAAAGCTTATCTCTGAAAGGT32840.19900473393721532No Hit
CTGGTATATTGAACAACTCTTGTATTTGGATACTACCTTAGCAGCATGTG32540.19718678569783762No Hit
GTATGTTCTTGCTCCAAGCAGTGATAATGGATAAAATGGAAGGAATGGGT31130.1886424289727623No Hit
GGTATATTGAACAACTCTTGTATTTGGATACTACCTTAGCAGCATGTGCC29970.18161302911383506No Hit
ATCTAATACAGTCCACCCCTGGTACTAGTGCTGACCCTCTTGATCCCACA29060.17609858612105594No Hit
ATATTGAACAACTCTTGTATTTGGATACTACCTTAGCAGCATGTGCCTGT28000.169675169008588No Hit
GAAAAAGAACTCTTGCTAATATTTAATTCCTTTTCTCCTAAGAAAAGCTT26040.15779790717798683No Hit
GTATTGTGGGGTTACTGGTATATTGAACAACTCTTGTATTTGGATACTAC25630.15531337791750394No Hit
CCTTCCTACAGTAAATTGTATTGTGGGGTTACTGGTATATTGAACAACTC23380.14167876612217095No Hit
GTCATAGAGAGGTGCCCGTGGTTTCTGAGGGCCAGGCTGTGGGCCGCAGC23250.14089098855177395No Hit
GTGATAATGGATAAAATGGAAGGAATGGGTGAGTCATTCAAACATACTCC22090.13386158869284673No Hit
ATATAATATTGGGTTCTCACATAGCAGGAGTGAATAATCTTTGTGAGAAT21480.13016509393944536No Hit
ACCTTAGCAGCATGTGCCTGTTTTTGATAAGGTAATTTCTGATATGTGAC20780.12592321471423065No Hit
ATTATATTCCTTATTATGGATGAGAAAAAGAACTCTTGCTAATATTTAAT20270.12283270270728852No Hit
ATACTGGATTGCTCCATAAGTGGAACTCTACCCTGAAACAGCTTTGGAAA19010.11519732010190206No Hit
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA17610.10671356165147265No Hit
TTCTTGCTCCAAGCAGTGATAATGGATAAAATGGAAGGAATGGGTGAGTC17560.10641057027824305No Hit
ATATTATATGAGGCTAATGCCCAGATCTGCAGGGGGAACTTAACAGATAA17530.10622877545430527No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT17460.1058045875317838No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA17040.10325945999665496No Hit
AAGAAAAGCTTATCTCTGAAAGGTTTTGCAAAGTATGTTCTTGCTCCAAG17020.10313826344736314No Hit
ACAGTAAATTGTATTGTGGGGTTACTGGTATATTGAACAACTCTTGTATT16970.10283527207413351No Hit
GTTAATAAGAGAGCCCTTGGAGTAGGAGATGAGCAATGTGACTGGACAGG16800.10180510140515281No Hit
CTGAAAGGTTTTGCAAAGTATGTTCTTGCTCCAAGCAGTGATAATGGATA16510.10004775144042098No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)