FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGG484.037005887300253No Hit
GAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGT453.7846930193439863No Hit
AGCGAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCG383.1959629941127No Hit
AGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTC363.027754415475189No Hit
GTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCC322.6913372582001682No Hit
AAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCT312.607232968881413No Hit
CTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACC302.5231286795626575No Hit
ATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAA292.4390243902439024No Hit
TAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCA231.9343986543313711No Hit
GGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCA231.9343986543313711No Hit
GGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCA211.7661900756938604No Hit
GCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCA191.59798149705635No Hit
AAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGC181.5138772077375946No Hit
CCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCG171.4297729184188395No Hit
CCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGC171.4297729184188395No Hit
GTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCC151.2615643397813288No Hit
GCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCG151.2615643397813288No Hit
TTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGT141.1774600504625736No Hit
CACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCC141.1774600504625736No Hit
GTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGC141.1774600504625736No Hit
CTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGG131.0933557611438183No Hit
GCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCG131.0933557611438183No Hit
GCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGC121.0092514718250631No Hit
TGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTC121.0092514718250631No Hit
CGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCC110.9251471825063078No Hit
GGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGC100.8410428931875525No Hit
GGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAAC100.8410428931875525No Hit
GGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTC90.7569386038687973No Hit
AAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTG90.7569386038687973No Hit
GCAAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCG90.7569386038687973No Hit
AGCGAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCC90.7569386038687973No Hit
GGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGG90.7569386038687973No Hit
GAGCAAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGC80.6728343145500421No Hit
TTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCCC80.6728343145500421No Hit
TCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGAT80.6728343145500421No Hit
CAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACC80.6728343145500421No Hit
TCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAAC80.6728343145500421No Hit
GCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACATGCC80.6728343145500421No Hit
ACCTCGGGTTCCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGG70.5887300252312868No Hit
GTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGT70.5887300252312868No Hit
GAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACATGCCCGC70.5887300252312868No Hit
GGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCAT70.5887300252312868No Hit
ACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACATGCCCGCCA70.5887300252312868No Hit
CAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCG60.5046257359125316No Hit
GATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGG60.5046257359125316No Hit
GCGAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGG60.5046257359125316No Hit
CAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCAC60.5046257359125316No Hit
AGCAAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCC60.5046257359125316No Hit
CCGTGAAGCCGGGCTGGAGATAGCGCTGCAGGAAGGGCAGGTGCACTTCC60.5046257359125316No Hit
GCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTG60.5046257359125316No Hit
AAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTT50.42052144659377627No Hit
CCTCGGGTTCCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGC50.42052144659377627No Hit
GAGCAAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCC50.42052144659377627No Hit
AGCAAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCG50.42052144659377627No Hit
AGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTT50.42052144659377627No Hit
CAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGG50.42052144659377627No Hit
GGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGG50.42052144659377627No Hit
CACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGG50.42052144659377627No Hit
GCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTC50.42052144659377627No Hit
CCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAGATAG50.42052144659377627No Hit
GAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGC50.42052144659377627No Hit
GTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTG40.33641715727502103No Hit
GTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGA40.33641715727502103No Hit
CCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCA40.33641715727502103No Hit
GAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGACCTGTCCCACTACC40.33641715727502103No Hit
GTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCAT40.33641715727502103No Hit
AAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGG40.33641715727502103No Hit
GCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGACCTGTCCCA40.33641715727502103No Hit
AGCGAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGCTGTCTCTTATACA40.33641715727502103No Hit
GGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCA40.33641715727502103No Hit
CAAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGG40.33641715727502103No Hit
GCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCC40.33641715727502103No Hit
CGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCCCGACTG40.33641715727502103No Hit
GCGAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCG30.2523128679562658No Hit
AGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCAC30.2523128679562658No Hit
GAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGC30.2523128679562658No Hit
GCAAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGG30.2523128679562658No Hit
CGGCACCACCGTGAAGCCGGGCTGGAGATAGCGCTGCAGGAAGGGCAGGT30.2523128679562658No Hit
GGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTT30.2523128679562658No Hit
GGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGC30.2523128679562658No Hit
TTCCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAGAT30.2523128679562658No Hit
AGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGC30.2523128679562658No Hit
CCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACATGCCCGCCAC30.2523128679562658No Hit
GAGCGAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGC30.2523128679562658No Hit
CCATCCGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCG30.2523128679562658No Hit
GCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCC30.2523128679562658No Hit
CGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGACCT30.2523128679562658No Hit
TCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTC30.2523128679562658No Hit
ACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAGATAGCGCTG30.2523128679562658No Hit
GCCGCCATCCGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTC20.16820857863751051No Hit
GGCACCACCGTGAAGCCGGGCTGGAGATAGCGCTGCAGGAAGGGCAGGTG20.16820857863751051No Hit
GAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTG20.16820857863751051No Hit
ACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCCCGACT20.16820857863751051No Hit
CCGCCGCCATCCGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATC20.16820857863751051No Hit
TCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCC20.16820857863751051No Hit
TCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCG20.16820857863751051No Hit
GGTTCCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAG20.16820857863751051No Hit
GTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCTGTCTCTTATAC20.16820857863751051No Hit
TAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGCTGTCTCTTAT20.16820857863751051No Hit
GTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACG20.16820857863751051No Hit
GGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGACCTGTCCC20.16820857863751051No Hit
CAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGA20.16820857863751051No Hit
GCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCA20.16820857863751051No Hit
GCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGG20.16820857863751051No Hit
GCCATCCGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTC20.16820857863751051No Hit
CCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATG20.16820857863751051No Hit
CAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGT20.16820857863751051No Hit
TGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGC20.16820857863751051No Hit
GTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGG20.16820857863751051No Hit
TTCCCGGCCTGCGCCACGTCTTCAGGAGAACGGCATGTTCACCGGCATTA20.16820857863751051No Hit
CCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAGATCT20.16820857863751051No Hit
GGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGG20.16820857863751051No Hit
CTCGGGTTCCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCT20.16820857863751051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)