FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660335
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA36910.2223045349281922No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA36590.22037721303230975No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT32350.19484019791186719No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG27920.16815883541574442No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT25790.15533009904627681No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG25360.15274026024868476No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG23950.14424799814495268No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC23570.14195930339359228No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT22450.13521367675800366No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG21970.13232269391417997No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC21590.13003399916281955No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA21380.12876919416864668No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC19740.11889166945224909No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC19620.11816892374129316No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA19270.11606091541767173No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA18130.10919483116359047No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA17280.1040753823776527No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)