FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_F12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_F12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794
Sequences flagged as poor quality0
Sequence length151
%GC75

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGC1096.075808249721293No Hit
AGCGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGC965.351170568561873No Hit
GGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTT673.7346711259754737No Hit
ACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCG653.6231884057971016No Hit
AAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCG613.400222965440357No Hit
AGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGG573.177257525083612No Hit
GGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCAT422.341137123745819No Hit
CAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCC422.341137123745819No Hit
AGCGAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCG412.2853957636566333No Hit
GTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCC351.950947603121516No Hit
GCGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCC311.7279821627647716No Hit
AGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGG291.6164994425863992No Hit
GTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGAC281.560758082497213No Hit
GCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCT251.3935340022296545No Hit
CAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGC231.282051282051282No Hit
CCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCC231.282051282051282No Hit
CTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGC191.0590858416945375No Hit
GTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCC170.947603121516165No Hit
GCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGC160.8918617614269788No Hit
TGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGC140.7803790412486065No Hit
CCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGTA140.7803790412486065No Hit
GGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCG140.7803790412486065No Hit
GCGAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGT130.7246376811594203No Hit
CCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTC130.7246376811594203No Hit
CGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCT130.7246376811594203No Hit
GCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGT120.6688963210702341No Hit
TCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGC110.6131549609810479No Hit
CGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGC110.6131549609810479No Hit
GCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGC110.6131549609810479No Hit
AAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCG100.5574136008918618No Hit
GGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGC90.5016722408026756No Hit
GAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCG90.5016722408026756No Hit
CCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCT90.5016722408026756No Hit
TCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGAC80.4459308807134894No Hit
CAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCG80.4459308807134894No Hit
CTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGTAACGCCGCC80.4459308807134894No Hit
GTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGT80.4459308807134894No Hit
GCCACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCG80.4459308807134894No Hit
GGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACGGCGG80.4459308807134894No Hit
GATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACGGCGGG80.4459308807134894No Hit
GAGCGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCG70.39018952062430323No Hit
CGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCG70.39018952062430323No Hit
GCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAG70.39018952062430323No Hit
GCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGC70.39018952062430323No Hit
ACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTG70.39018952062430323No Hit
ATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCT70.39018952062430323No Hit
GCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCG60.33444816053511706No Hit
CATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGTAACGC60.33444816053511706No Hit
CCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGC60.33444816053511706No Hit
CCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGC60.33444816053511706No Hit
GCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGT60.33444816053511706No Hit
GGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCG60.33444816053511706No Hit
CTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCAC50.2787068004459309No Hit
ACGTCACCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGCGCCCGTCGGC50.2787068004459309No Hit
GAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGG50.2787068004459309No Hit
GAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGC50.2787068004459309No Hit
GAGCGAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGC50.2787068004459309No Hit
CCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAG50.2787068004459309No Hit
AGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCA40.2229654403567447No Hit
CCACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGG40.2229654403567447No Hit
CGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGG40.2229654403567447No Hit
GACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGT40.2229654403567447No Hit
AAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCC40.2229654403567447No Hit
GTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTG40.2229654403567447No Hit
CCGCCACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGC40.2229654403567447No Hit
GCGACGACGTCACCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGCGCCC30.16722408026755853No Hit
CCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGTAACGCCGCCGGCG30.16722408026755853No Hit
GGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTA30.16722408026755853No Hit
GAGACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGCTTTCCCGCCGTCGC30.16722408026755853No Hit
GGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGC30.16722408026755853No Hit
CGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGCGC30.16722408026755853No Hit
CTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAG30.16722408026755853No Hit
GGCCGGCACGCGACGGCGGGAAAGCAGGGGGAGACGCATCGGTTGTCCTG30.16722408026755853No Hit
CGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGCCC30.16722408026755853No Hit
GCGATGGCCGGCACGCGACGGCGGGAAAGCAGGGGGAGACGCATCGGTTG30.16722408026755853No Hit
GCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTC30.16722408026755853No Hit
GCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGT30.16722408026755853No Hit
CCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGG30.16722408026755853No Hit
GCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGA30.16722408026755853No Hit
CGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACG30.16722408026755853No Hit
ACCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGCGCCCGTCGGCACGAC30.16722408026755853No Hit
CTCGCCGCCACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGA30.16722408026755853No Hit
CTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTT20.11148272017837235No Hit
TTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTC20.11148272017837235No Hit
TTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCA20.11148272017837235No Hit
AAAGCAGGGCGCGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGC20.11148272017837235No Hit
CCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGCGCCCGTCGGCACGACG20.11148272017837235No Hit
TGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCC20.11148272017837235No Hit
CAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCCGCC20.11148272017837235No Hit
CCTGTGGCGGCGACGACGTCACCTCGCAGCCGGTAACGCCGCCGGCGGCC20.11148272017837235No Hit
GCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGA20.11148272017837235No Hit
AGCAGGGGGAGACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGCTTTCCC20.11148272017837235No Hit
GGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGG20.11148272017837235No Hit
TCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCACAGGCGGCGAC20.11148272017837235No Hit
GTCCTGCTTTCGCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGC20.11148272017837235No Hit
CGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGG20.11148272017837235No Hit
ACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCC20.11148272017837235No Hit
CGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGGGGCCCCCC20.11148272017837235No Hit
GTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCCGCCATCGCCGCCCTCGCC20.11148272017837235No Hit
AAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTTCCG20.11148272017837235No Hit
GGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGCC20.11148272017837235No Hit
GAGCAAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCG20.11148272017837235No Hit
GCAAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCC20.11148272017837235No Hit
CCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGC20.11148272017837235No Hit
AAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCT20.11148272017837235No Hit
GGCGGCGACGACGTCACCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGC20.11148272017837235No Hit
GGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACGGCGGGAAAGCA20.11148272017837235No Hit
CGCCGCCACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATG20.11148272017837235No Hit
GTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATAGCCGCGCTCGCC20.11148272017837235No Hit
CGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGGGG20.11148272017837235No Hit
GGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGG20.11148272017837235No Hit
GGGAGACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGCTTTCCCGCCGTC20.11148272017837235No Hit
GCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCA20.11148272017837235No Hit
CGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCT20.11148272017837235No Hit
GCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACG20.11148272017837235No Hit
ACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTC20.11148272017837235No Hit
AGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACGGCGGGAAAGCAGGGG20.11148272017837235No Hit
GTGGTGGCTTGGGTTTGCGGGTCGATGCTCTCGGTGCCCACCAGGGAAAT20.11148272017837235No Hit
GCAGGGGGAGACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGCTTTCCCG20.11148272017837235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)