FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_F03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_F03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824110
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT48970.26845968718991725No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA35050.1921484998163488No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG33410.1831578139476238No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA32570.17855282850266704No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA25440.13946527347583204No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC22440.12301889688670091No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC22140.1213742592277878No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA21280.11665963127223686No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG19460.10668216280816398No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA18910.10366699376682328No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG18720.1026253899161783No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)