FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_F01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_F01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880711
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG73860.39272381562079445No Hit
TTATAATACTGTGACCACCACCAGTTTCGAGAAATACTACCTTTTGCCTC66290.3524730806593889No Hit
GTCTATAAGATATCCAGGTAAATATATTGACAAGACAAATGGAAATGAGC35540.1889710859350533No Hit
GTGCTGGAGAACAGCCCCCACTGCGACACCATGGTGGACAACAACCTGCG34910.18562128896996932No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG32910.17498701289033775No Hit
GTGTATACTCTGCCTGAACGTAATAATAGCAGATATATTGGTGAACAAGG29430.1564833725117788No Hit
GAGTATACACTGGGTATTTTAAAATATGGATGATGGATTGGTATGCGTAT26870.14287149912985037No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA26780.14239295670626695No Hit
TCTTGATACAACCCATTTTGCCCAGGAAACTTGCTTCTGGCATCCGTTAT24010.12766448433597719No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC23710.12606934292403246No Hit
GTCCTAAGCGAGGACTAAGAGAGAATGAAGGCCACTGTCCCAGGTCACCC20970.11150038469493719No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)