FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5941
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTA4958.331930651405488No Hit
AGCGAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGC2494.191213600403972No Hit
GGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGC1833.0802895135499075No Hit
CTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACG1702.8614711328059252No Hit
CCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACG1352.2723447231105873No Hit
CTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCC1322.22184817370813No Hit
GGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCC1232.0703585255007573No Hit
TCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCT991.6663861302810976No Hit
GCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGG941.582225214610335No Hit
GGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTG801.3465746507321998No Hit
GGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGC751.2624137350614375No Hit
GAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGA691.1614206362565225No Hit
GTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGC601.00993098804915No Hit
GCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGG601.00993098804915No Hit
GTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGC570.9594344386466925No Hit
GCGAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCT570.9594344386466925No Hit
GCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAAC540.9089378892442349No Hit
GCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTG500.8416091567076249No Hit
CTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCA490.8247769735734725No Hit
CCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACG460.774280424171015No Hit
ACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACC420.706951691634405No Hit
CCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTA370.6227907759636424No Hit
CACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTG360.6059585928294899No Hit
GGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGC360.6059585928294899No Hit
CCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCT340.572294226561185No Hit
CCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCG340.572294226561185No Hit
CCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGC340.572294226561185No Hit
CACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCA340.572294226561185No Hit
TGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGG330.5554620434270325No Hit
ACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCT330.5554620434270325No Hit
GCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGG320.53862986029288No Hit
GTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCC320.53862986029288No Hit
GGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGA310.5217976771587275No Hit
GCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTT300.504965494024575No Hit
GGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGT290.4881333108904225No Hit
GCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGG270.45446894462211745No Hit
CCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCC270.45446894462211745No Hit
CCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGC260.437636761487965No Hit
CCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGAC250.42080457835381246No Hit
CCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTC250.42080457835381246No Hit
AAAGCAGGCTTCGGGCCCCCGCTCCTTCTCCCTGCCCCTCGACCTCACCC240.40397239521965994No Hit
CCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCC230.3871402120855075No Hit
CTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGC230.3871402120855075No Hit
ACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGC220.370308028951355No Hit
CTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTC220.370308028951355No Hit
AGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGT210.3534758458172025No Hit
CCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCCGCCCTGCCCTGC210.3534758458172025No Hit
GCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCT210.3534758458172025No Hit
GTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGGGGCAG210.3534758458172025No Hit
GAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCG190.3198114795488975No Hit
CTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTT180.30297929641474497No Hit
GAGCGAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAG180.30297929641474497No Hit
GCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTT170.2861471132805925No Hit
ACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCCCC170.2861471132805925No Hit
TGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGG160.26931493014644No Hit
CAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGA160.26931493014644No Hit
TCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCC150.2524827470122875No Hit
CGAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTG150.2524827470122875No Hit
ACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACAC150.2524827470122875No Hit
GGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGG150.2524827470122875No Hit
CGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAG150.2524827470122875No Hit
CTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCT140.235650563878135No Hit
CTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCCGCCCTGCCCT140.235650563878135No Hit
GTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGT140.235650563878135No Hit
CTCCTTCTCCCTGCCCCTCGACCTCACCCAGCCCGCCACCCTCCTGGCTT140.235650563878135No Hit
CCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGC140.235650563878135No Hit
GCAAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCT140.235650563878135No Hit
CCCCTCGACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTC140.235650563878135No Hit
CTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTC130.2188183807439825No Hit
AGCGAAAGCAGGCTTCGGGCCCCCGCTCCTTCTCCCTGCCCCTCGACCTC120.20198619760982997No Hit
AAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGT120.20198619760982997No Hit
GCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCCGCCCTGCCC120.20198619760982997No Hit
GGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACA120.20198619760982997No Hit
TCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGC120.20198619760982997No Hit
GCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACC120.20198619760982997No Hit
GAGCAAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAG120.20198619760982997No Hit
GCCCCTCGACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTT110.1851540144756775No Hit
GGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCG110.1851540144756775No Hit
AGCAAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGC110.1851540144756775No Hit
GCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGC110.1851540144756775No Hit
GCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTAGAGAC100.16832183134152498No Hit
GCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGA100.16832183134152498No Hit
GAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAG100.16832183134152498No Hit
CTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTC100.16832183134152498No Hit
GAAGTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGGGG100.16832183134152498No Hit
GCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTA100.16832183134152498No Hit
GAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGA90.15148964820737248No Hit
CGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAA90.15148964820737248No Hit
GCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGG90.15148964820737248No Hit
GGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCG90.15148964820737248No Hit
GCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAG90.15148964820737248No Hit
GAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGT90.15148964820737248No Hit
GAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAG90.15148964820737248No Hit
GGGAAGTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGG80.13465746507322No Hit
CGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACT80.13465746507322No Hit
TACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCCGCCCTGCCCTGCTT80.13465746507322No Hit
GTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTAGAGACCGA80.13465746507322No Hit
CACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCCCCCCTG80.13465746507322No Hit
TCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTG80.13465746507322No Hit
GAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTAGAGACCGAAG80.13465746507322No Hit
AGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGC80.13465746507322No Hit
GAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGG70.1178252819390675No Hit
CGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCG70.1178252819390675No Hit
CGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGC70.1178252819390675No Hit
ACCTCACCCAGCCCGCCCCCCTCCTGGCTTCGGTCTCTACTTCCCCCACC70.1178252819390675No Hit
CACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCT70.1178252819390675No Hit
CGACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCA70.1178252819390675No Hit
GAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAG70.1178252819390675No Hit
CGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCG70.1178252819390675No Hit
GCGAAAGCAGGCTTCGGGCCCCCGCTCCTTCTCCCTGCCCCTCGACCTCA70.1178252819390675No Hit
ACCTCACCCAGCCCGCCCCCCTCCTGGCTTCGGTCTCTACTTCCCCCCCC70.1178252819390675No Hit
GGGTGAGGTCGAGGGGCAGGGAGAAGGAGCGGGGGCCCGAAGCCTGCTTT70.1178252819390675No Hit
GGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCG70.1178252819390675No Hit
GCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGC70.1178252819390675No Hit
CCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCA60.10099309880491499No Hit
GGCTTCGGGCCCCCGCTCCTTCTCCCTGCCCCTCGACCTCACCCAGCCCG60.10099309880491499No Hit
AGTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGGGGCA60.10099309880491499No Hit
TCGCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCT60.10099309880491499No Hit
GCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCT60.10099309880491499No Hit
CTACTTCGCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACG60.10099309880491499No Hit
AGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAG60.10099309880491499No Hit
GCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGA60.10099309880491499No Hit
CCTTCTCCCTGCCCCTCGACCTCACCCAGCCCGCCACCCTCCTGGCTTCG60.10099309880491499No Hit
GGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCA60.10099309880491499No Hit
CTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTC60.10099309880491499No Hit
CCCTCGACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCC60.10099309880491499No Hit
CCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTC60.10099309880491499No Hit
CTTCGGGCCCCCGCTCCTTCTCCCTGCCCCTCGACCTCACCCAGCCCGCC60.10099309880491499No Hit
TCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCG60.10099309880491499No Hit
CAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGT60.10099309880491499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)