FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_D10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_D10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290572
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC126350.5516089430936901No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG118220.5161156252674004No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT112970.493195586080682No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT94310.4117312182284599No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC75660.3303105075937364No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA75170.32817130393630933No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA71740.31319687833432No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT65200.2846450580902936No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC60090.26233621994855433No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT52450.2289821057796917No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT50860.2220406081974284No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC49590.21649614157511748No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG48770.21291624974023957No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT48470.2116065332152842No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT47930.2092490434703646No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG47800.208681499642884No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC43040.1879006641135926No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT42980.18763872080860153No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG42610.1860234037611566No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT40540.17698635973896476No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA39820.17384304007907195No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA38460.1679056584992744No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC37590.16410748057690394No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT37510.16375822283691585No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA37040.16170633361448583No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA36140.1577771840396198No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA35270.15397900611724932No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT35100.15323683341977462No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC34110.14891476888742203No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA33640.14686287966499198No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC33230.145072933747553No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA31550.1377385212078031No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG31410.13712732016282397No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC31150.1359922325078627No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT30430.13284891284796985No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC30270.13215039736799367No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG29930.1306660519730443No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG29870.13040410866805321No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT28590.12481598482824377No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGAGGAATCCAGC27760.12119243577586734No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG27740.12110512134087031No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC27620.12058123473088819No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG27550.12027563420839861No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT27450.11983906203341349No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT26880.11735060063599835No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA26860.11726328620100131No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG26840.1171759717660043No Hit
GTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA26740.11673939959101919No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAG26640.11630282741603407No Hit
ACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTC26440.11542968306606385No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT26360.11508042532607575No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT26210.11442556706359808No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT26060.11377070880112042No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATT25490.11128224740370528No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT24630.1075277266988333No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTC24600.10739675504633776No Hit
CTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCC24540.10713481174134669No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA24170.10551949469390179No Hit
ACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCC24120.10530120860640924No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTC24080.10512657973641519No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT23740.1036422343414658No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT23730.10359857712396729No Hit
AGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCC23440.10233251781651047No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC23420.10224520338151344No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC23240.10145937346654023No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACT23100.10084817242156108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)