FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_D08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_D08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963069
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGTCACTACATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAA51430.5340219651966785No Hit
GTAATAGTCAGAAAGCTAATATACCTGGTTGAAGCTAATAATGAAATGTT49730.5163700627888552No Hit
CTGTAAATGTGTAATGTTGTAAGTCACTACATTTGTAGTAATCTGTTAAT49170.5105553184662781No Hit
CCATAACTGTAAATGTGTAATGTTGTAAGTCACTACATTTGTAGTAATCT47540.4936302590987769No Hit
GCCTCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAG47400.49217657301813267No Hit
CATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCAGAGAGCA47310.49124206053771846No Hit
CTGTTAATGCAGCCAGAAGAAAATAATGCAGAGAGCAGAGCTGCTGGTTC47030.4883346883764299No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT43990.45676893348243996No Hit
AAATGGAACTTGCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAG42590.4422320726759973No Hit
GGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGAGTTCTGATGATG41640.43236777427162537No Hit
CTTCTACACAATGGGGTGTTTGCTCTGCCGTGATTAGCACTGTAATAGTC41070.42644919522900226No Hit
GAAATGGAACTTGCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGA40910.42478783970826595No Hit
GACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGA40750.42312648418752963No Hit
GTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCAGAGAGCAGAGCT40330.41876542594559685No Hit
CTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTCA39320.40827811922094887No Hit
GACTATTACAGTGCTAATCACGGCAGAGCAAACACCCCATTGTGTAGAAG38200.3966486305757947No Hit
ATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGAGTTCT37690.39135305985344765No Hit
GTGTAATGTTGTAAGTCACTACATTTGTAGTAATCTGTTAATGCAGCCAG37640.3908338862532176No Hit
CCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGA36750.38159259616912183No Hit
CTCCTGGAACTAGTTCACCTCTTGACCTTCACAGCTACAAGAGCTTGAAC36120.3750510088062226No Hit
GGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAAT35030.36373302432120647No Hit
GTATATTAGCTTTCTGACTATTACAGTGCTAATCACGGCAGAGCAAACAC34550.35874895775899757No Hit
GAGCAGAGCTGCTGGTTCTCATTTCATCTTCTACACAATGGGGTGTTTGC34470.3579182799986294No Hit
CCACTAAAGCCTCCAGAAGGAACATAGTCCTGCGGACACCTTGACTTGAA33460.3474309732739814No Hit
CTCCATAACTGTAAATGTGTAATGTTGTAAGTCACTACATTTGTAGTAAT32710.3396433692705299No Hit
AAAGCAGGCAGCCTCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATC31770.32988290558620414No Hit
GTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAA30500.3166958961403596No Hit
GTTCTGATGATGACAAAGGCTGCTGGCCCATCTCTCCTGGAACTAGTTCA28800.29904399373253626No Hit
GGGAAATGGAACTTGCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATA28250.29333308413000525No Hit
GTGGACATCTGACCTCCATAACTGTAAATGTGTAATGTTGTAAGTCACTA28210.29291774524982117No Hit
CATAAGCATATGTCACTGTGGCCCAGGCCTCGCCCATGGGTCCAGAGCCA28110.2918793980493609No Hit
ATCTTCTAGTTTCCCAAGTTGGAAGGGTGTTTTTAAGGGTAGGGGAGTCA27780.28845285228784234No Hit
GAAAAAGGGAGAGAGAGAGAGATGAGTTCTGATGATGACAAAGGCTGCTG27620.286791496767106No Hit
CCTCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAGC26500.2751620081219518No Hit
CTATACGGGAATCGGCACTTTTCTCCCACACTGTTGGCAAACCACTATAA26460.2747466692417677No Hit
GTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGC24940.25896379179477275No Hit
GTCCTGCGGACACCTTGACTTGAACCCAGCGAGACCCACTGTGGACATCT24690.2563679237936223No Hit
GACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGA24550.254914237712978No Hit
CTATAAGGCATTTCATGCAAAAAGACACGTTCAACATTTTTGCTTTCCCA24380.25314904747219563No Hit
AAAGCAGGCAGAGGGCTTAGCTGCTGAAACTGTGGAGCAGGGAGAGAGCA24270.25200686555168944No Hit
GTGTAGAAGATGAAATGAGAACCAGCAGCTCTGCTCTCTGCATTATTTTC22520.233835789543636No Hit
CTTCACCACCATTCTCAAAAGACAACATCAGGACCCGTGGCCCCTTCTGG22190.23040924378211736No Hit
GGCTAATACTCTATAACGGAAACTAAGGTTCCTAATCAGGGCCTCTTGCT22130.22978623546184127No Hit
GATTAGCACTGTAATAGTCAGAAAGCTAATATACCTGGTTGAAGCTAATA22110.2295785660217492No Hit
CTACATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCAGAGA22080.22926706186161117No Hit
AGCGAAAGCAGGGAAATGGAACTTGCCCATGGGTAGTGTCCTTCTGGGCC21860.22698269802059876No Hit
GCTACAAGAGCTTGAACATTTCATTATTAGCTTCAACCAGGTATATTAGC21410.22231013561852786No Hit
GACAAAGGCTGCTGGCCCATCTCTCCTGGAACTAGTTCACCTCTTGACCT21220.22033727593765345No Hit
AGCTTGAACATTTCATTATTAGCTTCAACCAGGTATATTAGCTTTCTGAC21130.21940276345723933No Hit
CCTCTTGACCTTCACAGCTACAAGAGCTTGAACATTTCATTATTAGCTTC21060.21867592041691716No Hit
ACTGTGGACATCTGACCTCCATAACTGTAAATGTGTAATGTTGTAAGTCA20900.21701456489618084No Hit
GCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAG20360.2114074900136958No Hit
CATCAGGACCCGTGGCCCCTTCTGGCCTCTGGTGTGAACCAGGGAGTGTG20150.20922696089272938No Hit
GCTAATATACCTGGTTGAAGCTAATAATGAAATGTTCAAGCTCTTGTAGC19580.20330838185010627No Hit
GGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATG19410.20154319160932394No Hit
GAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGA19210.19946649720840354No Hit
GGGCTTAGCTGCTGAAACTGTGGAGCAGGGAGAGAGCAGAAGGTGTTTGG19180.1991549930482655No Hit
ATAATGCAGAGAGCAGAGCTGCTGGTTCTCATTTCATCTTCTACACAATG19130.19863581944803538No Hit
CCACTAAATCTCCACTAAAGCCTCCAGAAGGAACATAGTCCTGCGGACAC18750.19469010008628665No Hit
ACATAGTCCTGCGGACACCTTGACTTGAACCCAGCGAGACCCACTGTGGA18650.19365175288582645No Hit
GAACATAGTCCTGCGGACACCTTGACTTGAACCCAGCGAGACCCACTGTG18630.1934440834457344No Hit
ATTTGATACTATACGGGAATCGGCACTTTTCTCCCACACTGTTGGCAAAC18510.19219806680518217No Hit
TTACAGTGCTAATCACGGCAGAGCAAACACCCCATTGTGTAGAAGATGAA18430.191367389044814No Hit
GAACTTGCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAAT18070.18762933912315732No Hit
AAATAATGCAGAGAGCAGAGCTGCTGGTTCTCATTTCATCTTCTACACAA17620.18295677672108646No Hit
CCCTTAGCCCTCTCTCTAGTCCTCGTCCCCGCCGAGTTAAAGTGTCTCCA17570.18243760312085636No Hit
CTCTAGTCCTCGTCCCCGCCGAGTTAAAGTGTCTCCAGAGTAACAACTCT17550.18222993368076432No Hit
ATACGGGAATCGGCACTTTTCTCCCACACTGTTGGCAAACCACTATAAGG17550.18222993368076432No Hit
CATCTGACCTCCATAACTGTAAATGTGTAATGTTGTAAGTCACTACATTT17500.1817107600805342No Hit
GTGCTAATCACGGCAGAGCAAACACCCCATTGTGTAGAAGATGAAATGAG17490.1816069253604882No Hit
ATTTCAGAGTCTTCATTTGGAAGAGATTTATTTGATACTATACGGGAATC17130.17786887543883148No Hit
GGCGTGGAGAATGCCTATCTTCACCACCATTCTCAAAAGACAACATCAGG16550.17184646167616235No Hit
TGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAG16330.16956209783514994No Hit
ACACAATGGGGTGTTTGCTCTGCCGTGATTAGCACTGTAATAGTCAGAAA16220.1684199159146437No Hit
GAAGAAAATAATGCAGAGAGCAGAGCTGCTGGTTCTCATTTCATCTTCTA16200.16821224647455166No Hit
GACTTGAACCCAGCGAGACCCACTGTGGACATCTGACCTCCATAACTGTA15770.16374735351257283No Hit
CTGTAATAGTCAGAAAGCTAATATACCTGGTTGAAGCTAATAATGAAATG15540.16135915495151437No Hit
GTTAATGCAGCCAGAAGAAAATAATGCAGAGAGCAGAGCTGCTGGTTCTC15440.1603208077510542No Hit
CTATTACAGTGCTAATCACGGCAGAGCAAACACCCCATTGTGTAGAAGAT15430.16021697303100815No Hit
GTGTTTGGGGGCGGTATCTTCTAGTTTCCCAAGTTGGAAGGGTGTTTTTA15030.15606358422916738No Hit
GTTTTTAAGGGTAGGGGAGTCAGTCAGGCAGGAATGGCTTTTGCCCCTGA14950.15523290646879923No Hit
ACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGA14940.1551290717487532No Hit
GCATATGTCACTGTGGCCCAGGCCTCGCCCATGGGTCCAGAGCCAGTCTC14940.1551290717487532No Hit
GGGTAGGGGAGTCAGTCAGGCAGGAATGGCTTTTGCCCCTGACCTGCAGG14910.15481756758861515No Hit
CATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACC14790.1535715509480629No Hit
GCCGTGATTAGCACTGTAATAGTCAGAAAGCTAATATACCTGGTTGAAGC14780.1534677162280169No Hit
GTAATGTTGTAAGTCACTACATTTGTAGTAATCTGTTAATGCAGCCAGAA14550.15107951766695846No Hit
GCACTGTAATAGTCAGAAAGCTAATATACCTGGTTGAAGCTAATAATGAA14420.14972966630636017No Hit
GTGTGTGTGTGTGCACACATAAGCATATGTCACTGTGGCCCAGGCCTCGC14390.14941816214622214No Hit
CTTCTGGCCTCTGGTGTGAACCAGGGAGTGTGTGTGTGTGCACACATAAG14330.14879515382594602No Hit
TGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAA14080.14619928582479552No Hit
GTGAAAAAGGGAGAGAGAGAGAGATGAGTTCTGATGATGACAAAGGCTGC14040.14578394694461144No Hit
CTACAAGAGCTTGAACATTTCATTATTAGCTTCAACCAGGTATATTAGCT13930.14464176502410525No Hit
AGCGAAAGCAGGCAGCCTCTAGAAGGTGAAAATGGCAAAAATCTCCACTA13890.14422642614392117No Hit
TTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTCAA13880.14412259142387512No Hit
GAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGA13820.143499583103599No Hit
CTGTGGACATCTGACCTCCATAACTGTAAATGTGTAATGTTGTAAGTCAC13770.14298040950336893No Hit
GTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGG13640.14163055814277067No Hit
GGCAGAGGGCTTAGCTGCTGAAACTGTGGAGCAGGGAGAGAGCAGAAGGT13620.14142288870267863No Hit
AAAGCAGGGCGTGGAGAATGCCTATCTTCACCACCATTCTCAAAAGACAA13430.13945002902180426No Hit
AGAGAGATGAGTTCTGATGATGACAAAGGCTGCTGGCCCATCTCTCCTGG13380.13893085542157416No Hit
CTATAACGGAAACTAAGGTTCCTAATCAGGGCCTCTTGCTTTGATAGAAG13340.13851551654139008No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA13060.13560814438010155No Hit
GATGAGTTCTGATGATGACAAAGGCTGCTGGCCCATCTCTCCTGGAACTA13060.13560814438010155No Hit
GAGAGAGAGAGATGAGTTCTGATGATGACAAAGGCTGCTGGCCCATCTCT12790.132804606938859No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG12720.13207776389853687No Hit
CTAATATACCTGGTTGAAGCTAATAATGAAATGTTCAAGCTCTTGTAGCT12620.13103941669807667No Hit
GATGATGACAAAGGCTGCTGGCCCATCTCTCCTGGAACTAGTTCACCTCT12530.1301049042176625No Hit
GGGAGAGAGAGAGAGATGAGTTCTGATGATGACAAAGGCTGCTGGCCCAT12530.1301049042176625No Hit
GGTATATTAGCTTTCTGACTATTACAGTGCTAATCACGGCAGAGCAAACA12460.12937806117734035No Hit
AATCACGGCAGAGCAAACACCCCATTGTGTAGAAGATGAAATGAGAACCA12390.12865121813701821No Hit
ACATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCAGAGAGC12360.12833971397688018No Hit
GTAAATGTGTAATGTTGTAAGTCACTACATTTGTAGTAATCTGTTAATGC12140.12605535013586772No Hit
GTATCTTCTAGTTTCCCAAGTTGGAAGGGTGTTTTTAAGGGTAGGGGAGT12110.12574384597572968No Hit
GTGTTTAGCCAGATATTCAAGTAATAACATTTCCCCTATTTCAGAGTCTT12090.12553617653563764No Hit
GTCCAGAGCCAGTCTCAGGAAGCGAGAGCCCGACTGGCTCCAGGATGAAC12040.12501700293540752No Hit
GCTGAAACTGTGGAGCAGGGAGAGAGCAGAAGGTGTTTGGGGGCGGTATC11960.12418632517503937No Hit
GTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGAGTTCTGATGATGA11910.12366715157480929No Hit
AGCGAAAGCAGGGCGTGGAGAATGCCTATCTTCACCACCATTCTCAAAAG11800.12252496965430305No Hit
GACGAGTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGAGTCTAGT11640.12086361413356675No Hit
CACATAAGCATATGTCACTGTGGCCCAGGCCTCGCCCATGGGTCCAGAGC11630.12075977941352074No Hit
ATACTATACGGGAATCGGCACTTTTCTCCCACACTGTTGGCAAACCACTA11520.11961759749301451No Hit
GCAGAGAGCAGAGCTGCTGGTTCTCATTTCATCTTCTACACAATGGGGTG11490.11930609333287646No Hit
GTTCAAGCTCTTGTAGCTGTGAAGGTCAAGAGGTGAACTAGTTCCAGGAG11440.11878691973264635No Hit
GGCCTAGCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGGGATCC11410.1184754155725083No Hit
GTGTGTGTGTGCACACATAAGCATATGTCACTGTGGCCCAGGCCTCGCCC11380.11816391141237025No Hit
GGAGAGAGCAGAAGGTGTTTGGGGGCGGTATCTTCTAGTTTCCCAAGTTG11280.11712556421191005No Hit
CCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAA10990.1141143573305755No Hit
CTATAGGCTTTTCTGAGAGGTCTGTTGGTTTGTCACACAGCCGCCACAGT10980.11401052261052946No Hit
CAATGGGGTGTTTGCTCTGCCGTGATTAGCACTGTAATAGTCAGAAAGCT10920.11338751429025334No Hit
CCTTGACTTGAACCCAGCGAGACCCACTGTGGACATCTGACCTCCATAAC10820.11234916708979314No Hit
TGCCTATCTTCACCACCATTCTCAAAAGACAACATCAGGACCCGTGGCCC10820.11234916708979314No Hit
CTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAGCCTC10790.11203766292965509No Hit
CAGATAGAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAA10720.11131081988933296No Hit
CTACACAATGGGGTGTTTGCTCTGCCGTGATTAGCACTGTAATAGTCAGA10690.11099931572919489No Hit
GTCACTACATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCA10660.11068781156905684No Hit
TTTTAAGGGTAGGGGAGTCAGTCAGGCAGGAATGGCTTTTGCCCCTGACC10620.11027247268887276No Hit
GGCCCATCTCTCCTGGAACTAGTTCACCTCTTGACCTTCACAGCTACAAG10610.11016863796882673No Hit
TTTCATTATTAGCTTCAACCAGGTATATTAGCTTTCTGACTATTACAGTG10560.10964946436859664No Hit
AGTAATAACATTTCCCCTATTTCAGAGTCTTCATTTGGAAGAGATTTATT10510.10913029076836654No Hit
TGACAAAGGCTGCTGGCCCATCTCTCCTGGAACTAGTTCACCTCTTGACC10480.1088187866082285No Hit
CCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGAGT10420.10819577828795238No Hit
GAGCAGGGAGAGAGCAGAAGGTGTTTGGGGGCGGTATCTTCTAGTTTCCC10310.10705359636744614No Hit
CTTAATGAGGGACCCTAATTCGGTCCTCGCCTAATCAAGGCCTCCCTTAG10290.1068459269273541No Hit
GATTTATTTGATACTATACGGGAATCGGCACTTTTCTCCCACACTGTTGG10260.10653442276721606No Hit
GTGTGGGGCTCAATCTCAGGACCCTGGGATCATGACCTGAGCCAAAAGCA10250.10643058804717002No Hit
CCCTAATTCGGTCCTCGCCTAATCAAGGCCTCCCTTAGCCCTCTCTCTAG10170.10559991028680188No Hit
ATCTAAATCCCTCTGTTCCCCCTCCCCCCCAGTGATGGAATTTGTTAAAA10070.10456156308634168No Hit
GAAGGAACATAGTCCTGCGGACACCTTGACTTGAACCCAGCGAGACCCAC10040.10425005892620363No Hit
TTCATGATCCGCTCCATGTTGGCTGTCCAGCCATAGGTGCTAGTCACAAT10000.10383472004601955No Hit
GAGCAGAAGGTGTTTGGGGGCGGTATCTTCTAGTTTCCCAAGTTGGAAGG9930.1031078770056974No Hit
CATTTCATCTTCTACACAATGGGGTGTTTGCTCTGCCGTGATTAGCACTG9900.10279637284555936No Hit
CTCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAGCC9880.10258870340546732No Hit
GATAGAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGG9880.10258870340546732No Hit
CTATATCCCTTGGGTCTCACTTCCATCCACCAGCCAGGACACCTGGGCAC9790.10165419092505314No Hit
CTCCAGAAGGAACATAGTCCTGCGGACACCTTGACTTGAACCCAGCGAGA9750.10123885204486906No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)