FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_D04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_D04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786168
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA49250.27572994253620037No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA38460.21532129116634047No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT35900.20098893273197146No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT33700.1886720622024356No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT31440.17601927702209422No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC30560.1710925288102799No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC29700.16627775214873405No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG28430.1591675587066838No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG27950.15648024150023962No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC26970.15099363553708273No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC25770.14427534252097227No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA25700.14388344209503248No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG25670.14371548476962973No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA25070.14035633826157448No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG24900.13940458008429218No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA22800.12764756730609886No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA22290.12479229277425191No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC21720.12160110359159945No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA21060.11790604243273868No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA20300.1136511235225354No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC19320.10816451755937852No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC19240.10771663135830448No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA19180.10738071670749896No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA18180.10178213919407357No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA17970.10060643791625423No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)