FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_C05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_C05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536201
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC72980.47506804122637597No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG62250.40522041061033026No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA37910.2467776026704839No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA31650.20602772684043297No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA30420.1980209621006626No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA30160.19632847524510139No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT29290.1906651538438004No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT28030.18246310215915754No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC27620.1797941805792341No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC26750.1741308591779331No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG26530.17269875491553513No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA25970.16905339861124943No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA23700.15427668644923417No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC23670.15408139950436175No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT23590.1535606343180352No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT23460.1527143908902546No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT22670.14757183467528012No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT22280.14503310439193828No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA22190.14444724355732094No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT21190.13793767872823934No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA20390.13273002686497404No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA20050.1305167748230863No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT19310.12569969684956592No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC19260.12537421860811185No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA19080.12420249693887712No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC18960.12342134915938735No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA18240.1187344624824486No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC18040.11743254951663226No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA17470.11372209756405574No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA17230.11215980200507615No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT16940.1102720282046425No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG16800.10936068912857105No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT16570.1078634892178823No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT16410.10682195884522923No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT15840.10311150689265272No Hit
GAAATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATT15780.10272093300290781No Hit
AATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGA15720.10233035911316292No Hit
GTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATA15540.10115863744392824No Hit
ATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATTCTT15480.10076806355418333No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)