FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508673
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT78970.5234401358014626No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC60060.39809819622940157No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA59970.39750164548580114No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA57580.38165990907241No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG49180.32598183966969646No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA42780.28356045345810527No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG37810.2506175957281664No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA35990.23855401402424514No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC35340.23424559198713038No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG34580.22920805237450398No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA32810.21747588775036072No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG32240.2136977330408909No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT28170.1867203827469571No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG27860.18466559685233314No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA27420.18174912655028624No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT25180.16690164137622931No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA24530.16259321933911458No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA23520.15589859432759784No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA23430.15530204358399732No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC23400.15510319333613048No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG23010.15251814011386164No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT21680.14370244579176536No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA21150.14018942474611795No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC20710.13727295444407103No Hit
GTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAA20710.13727295444407103No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA20380.13508560171753586No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG20190.13382621681437926No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA20160.13362736656651242No Hit
GTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCT18780.12448025516463807No Hit
CAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTA18030.11950899896796721No Hit
GGTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATC17940.11891244822436671No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA17640.11692394574569837No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC17360.11506801009894127No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA16530.10956648657462552No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA16390.10863851875124696No Hit
CTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAA16260.10777683434382401No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT15900.10539063136942199No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG15780.10459523037795466No Hit
GATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATATTA15450.10240787765141948No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC15180.10061822542061799No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)