FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248211
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG122540.9817250448842383No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA64790.5190628827978603No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT63520.5088883209649651No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA59510.4767623422642486No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA58170.46602697781064256No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT48350.38735438159093294No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT47750.38254750198484067No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC45360.3634000982205733No Hit
TTTTAATACATCAGGCATTTTTCTCAATGACTTCAGATAGGTAAGTCTCC41980.33632134310625367No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC41720.33423836194361367No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT40430.32390357079051535No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA40120.3214200163273677No Hit
AATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGA37920.3037947911050295No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG35380.28344566743923905No Hit
ATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATC31570.2529219819405533No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT30780.24659292379253187No Hit
GAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAA28930.23177171167374747No Hit
GCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGT28700.22992907449141212No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA28470.22808643730907677No Hit
ATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGT28320.2268847174075537No Hit
TTTTTTAATACATCAGGCATTTTTCTCAATGACTTCAGATAGGTAAGTCT28210.22600345614643677No Hit
AGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTC28130.22536253886562446No Hit
ATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAA26740.2142266011115108No Hit
GTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATA26560.2127845372296831No Hit
GCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCTGAGCCGA26140.20941972150541857No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT25940.20781742830338779No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT25280.20252986073668633No Hit
ACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCAT24080.19291610152450187No Hit
TAGTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGA23060.18474440619414506No Hit
GATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGG21920.1756113349425698No Hit
ATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCTGAGCCGAAGGCA21280.1704839966960714No Hit
GTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTT20990.16816067155312683No Hit
TTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATT20980.1680805568930253No Hit
GGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTT20700.16583734641018227No Hit
CCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTCAATGACTTCA20550.16463562650865918No Hit
TCCCAGGACCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCA20490.16415493854804997No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG19910.1595082882621608No Hit
AAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAG19700.1578258804000285No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT19420.15558266991718547No Hit
TCCCTATCCTATGGGGAATTAATGGAAGATCCAGAACTAGAGCCTAGGTT19250.15422072069545936No Hit
AGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAGCA19240.15414060603535779No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG18830.15085590497119478No Hit
GGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAGAGACAA18280.14644959866561022No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA18050.14460696148327487No Hit
CCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGATC17820.14276432430093952No Hit
TCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAAT17750.14220352168022873No Hit
GGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATC17320.13875859129586265No Hit
TCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTTAA16980.13603469285241038No Hit
ATATCACACTGTATGTTAATTACACTGGAATTAAAAACAAAGATATGCAT16930.1356341195519027No Hit
TTCCTATACAGATGGCTCTAATTTGGGAAGTACACTGACTTTTTGACTTG16830.1348329729508873No Hit
CCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCAT16720.13395171168977038No Hit
CCATGAATGATGTCCAAGTCAGTAGTTCAAAGCTTACAGTGAGCGGCATT16670.1335511383892627No Hit
ATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCATTC16630.13323067974885655No Hit
ATCCCAGGACCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCC16190.12970563470438892No Hit
CTGTAAGCTTTGAACTACTGACTTGGACATCATTCATGGAGACTTACCTA16030.12842380014276433No Hit
GGTAAGTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAAGCTTACAGTG15860.1270618509210382No Hit
CCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCA15790.12650104830032743No Hit
GTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAAGCTTACAGTGAGCGG15710.12586013101951513No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC15550.12457829645789054No Hit
TATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAAT15540.12449818179778899No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA15340.12289588859575826No Hit
CATCAGGCATTTTTCTCAATGACTTCAGATAGGTAAGTCTCCATGAATGA15270.12233508597504748No Hit
GGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAAT15130.12121348073362596No Hit
CCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGG15090.12089302209321981No Hit
GGCTAATAGAAGGTAGAAAGTCAATTAGAGACGATTACAAACTAACTGAC15020.12033221947250906No Hit
CTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAA14740.11808900898966602No Hit
GTTCAATGGCTGAGCCAAGCCTTGAATGGCATGTCAGGTAGGCCAGGAAA14680.11760832102905679No Hit
GAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCATGTTGGCTGT14510.11624637180733063No Hit
GAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTT14420.1155253398664168No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT14340.11488442258560452No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG14000.11216052414215225No Hit
GTTCTGCATTTTTAAAATTAAATTCCTTAGAGACAAGCTAATTTGCCAGA13910.1114394922012384No Hit
GTTCAAAGGCGCGCAAAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCC13580.10879570841788769No Hit
AGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCC13520.10831502045727846No Hit
TTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCA13460.10783433249666922No Hit
CTTCAGATAGGTAAGTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAAG13400.10735364453606001No Hit
ACCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCC13380.10719341521585693No Hit
GGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGTGGGACT13240.10607180997443541No Hit
GAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTCAAAAG13240.10607180997443541No Hit
TCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTCAAAAGCTT13200.10575135133402926No Hit
AGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCT13090.10487009007291236No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA13080.10478997541281082No Hit
GCTTTAGAATTCAGTCATGTGAAATTAAACTCAAAAGCTTAGAGAACCTA12830.10278710891027237No Hit
ATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAG12750.10214619162946008No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA12500.10014332512692165No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)