FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456624
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG60650.41637375190852266No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC53730.36886663957205157No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA33310.22867946704159758No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA27650.18982249365656478No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA25790.17705324091872712No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA25490.1749936840255275No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT24240.1664121969705291No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT23440.16092004525533013No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC23100.15858588077637056No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC22540.15474137457573128No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT22270.1528877733718516No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT22080.15158338733949187No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT21990.15096552027153198No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA21900.1503476532035721No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA21790.14959248234273226No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA21730.14918057096409232No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT21520.14773888113885258No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA20980.14403167873109327No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT20960.1438943749382133No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT19550.1342144575401751No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC19270.13229220443985545No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG19030.13064455892529575No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG18430.12652544513889652No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT17720.12165116049165743No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG16950.1163649644657784No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA16800.1153351860191786No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC16780.1151978822262986No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC16420.11272641395445908No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG16230.11142202792209933No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC16060.11025494568261954No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA15980.10970573051109964No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA15900.10915651533957975No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG15840.10874460396093982No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT15490.10634178758554026No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT15190.10428223069234065No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA15180.10421357879590067No Hit
GAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAA15040.10325245224574084No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)