FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585265
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT75090.47367474838591656No Hit
ACATAAGGGTGCATAGATCTTTCTAAACTTATGTTTTTGTTTTGTTTAAT34300.21636761046260403No Hit
GTTCTTAGGTGTGACAGAAGAAAATTAATGAACGTGCCCTTCCACATTTG30820.194415444736369No Hit
CCTCTAGGGTGCGCCTGTCCCTGAGTGCCAAGACTTATGAGCTAGATGGC27680.17460803083396154No Hit
AAACATAAGGGTGCATAGATCTTTCTAAACTTATGTTTTTGTTTTGTTTA24830.15662996407540694No Hit
ATTTATGAGTCTGTCTGCTTTGTCTATTTTTTCTTAGATTCTTCATGAAA24790.15637764033142723No Hit
TTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCAAACAAGGCAAAGGAT23980.15126808451583806No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA22680.14306756283649738No Hit
CCTATGGATGGACCCTTGCATTACTTCCATATCTTGGCTATTGTAACTAA22430.14149053943662415No Hit
GCATTAAACAAAGCAAACAAGGCAAAGGATTTGAATAGATATTTCTCCAA22330.14085973007667488No Hit
GAATATTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCAAACAAGGCAA22240.14029200165272052No Hit
ATTGTAACTAATGCTGCAGTAAACATAAGGGTGCATAGATCTTTCTAAAC22210.14010275884473575No Hit
TCCCTGAACCATGAGGGCATGGGAAGGCTGCCATAGCTCTGGCTCCACTC21800.13751644046894368No Hit
TCTATGTGTTGAATATTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCA20660.13032521376552186No Hit
GTCTGGGGCGGTCTGTGAGCAGGATCAGCTGGGCATGGAATGGGGCCCAC19680.12414328203801889No Hit
GTGTTGAATATTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCAAACAA19600.12363863455005947No Hit
AACTATACCCATCCATGAAATCATTCAGCTTCAGCATTATAGCAGATGTT19470.1228185823821254No Hit
GTTGAATATTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCAAACAAGG19370.1221877730221761No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG19290.12168312553421667No Hit
GTCCATATCCACTGCTCCCTTTCCTTGGTACCTGGTGGAGTTTGGCCAGC17890.11285179449492672No Hit
CATTTATTGAGAATCTATGTGTTGAATATTGTAGGTCCAAGTGATAGAGC17790.11222098513497744No Hit
TCTTTATCCATTCACCTATGGATGGACCCTTGCATTACTTCCATATCTTG16780.10584981059948968No Hit
ACCAAGGGCCATTCCCTGAACCATGAGGGCATGGGAAGGCTGCCATAGCT16540.1043358681356114No Hit
GACGAGTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGAGTCTAGT16470.10389430158364689No Hit
GTCTACCTCACCCTGAATGGTAACAGACTGAACGTTTTTCCTACAAGATC16420.10357889690367225No Hit
GTACATGGGGGTTAGGGAGCAGGTCTGGTTAGTAGAGGAATTTAGACTCA16330.1030111684797179No Hit
CCCCTAGGCTGGAGTTGCCATCAGTGAGAAGGGGAAGTACATGGGGGTTA16040.10118182133586498No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)