FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_A10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_A10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355389
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT91680.6764109786932018No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA64610.476689717859596No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA63920.471598928425714No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC58060.4283641080162226No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG54710.4036479564169401No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA48040.3544369918894133No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA43260.31917036363730267No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC40840.301315710840209No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA39850.2940115346959434No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG38430.2835348376001281No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG33830.24959624137424757No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG33200.24494812928244217No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG31310.23100379300702603No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA27960.20628764140774347No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT27820.20525472760956448No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT27710.20444315248242384No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA27250.2010492928598358No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT24060.1775136141727578No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC22790.16814361043213424No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA22710.16755337397603198No Hit
GTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAA22460.16570888505071238No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA22310.16460219169552062No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA22300.16452841213850783No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC22120.16320038011227772No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG22050.16268392321318825No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA21440.15818337023540843No Hit
GTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCT20600.1519858874463346No Hit
GGTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATC20220.14918226427984882No Hit
CAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTA20200.14903470516582326No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG20010.14763289358258033No Hit
CTTCTAATTGTGGTAAAATACAATAACACAAAACTCACACCTTAATCATT18880.13929580364013577No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA18740.1382628898419568No Hit
ACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGAGTTTGT18270.13479525066235595No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA17540.12940934300042276No Hit
ACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTT17320.1277861927461415No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC16990.12535146736471964No Hit
GATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATATTA16860.12439233312355345No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA16290.12018689837382478No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG16280.120113118816812No Hit
CTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAA16060.11848996856253075No Hit
CCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCC16000.11804729122045406No Hit
GTTCTAATAGTTACCCTAGCTAGAAACCTCAAAACATTTTTAGTTCCTTC15640.11539122716799383No Hit
TTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCT15300.11288272222955918No Hit
GTAAAATACAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACG15050.1110382333042396No Hit
ACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACA15050.1110382333042396No Hit
TCCTAAGAGTAATCTTGGAGACTTACACAGAGGGCATTAGAGCCTTATCA15020.11081689463320124No Hit
CCTCCAAATAGCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAA14690.10838216925177938No Hit
GCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAATGGATAAACA14610.1077919327956771No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA14330.10572610519931916No Hit
GTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATC14320.10565232564230638No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA13800.10181578867764161No Hit
GCATATTACTTGGTCTTCAGATGGATGGAAATCCTGACACGGGCTACAGC13560.10004507930933482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)