FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536057
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC68280.44451475433528836No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA47960.31222799674751656No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT46970.30578292342015956No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT45310.2949760327904498No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA45040.2932182855193525No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC38940.25350621754270836No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG37370.2432852426700311No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT36170.23547303257626506No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA34070.22180166491217448No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA33980.22121574915514203No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG33700.21939290013326326No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG31880.20754438149105142No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA29670.19315689456836563No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG29160.18983670527851507No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT28660.18658161773944587No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG27590.1796157304058378No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC26870.17492840434957815No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC26670.1736263693339505No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT26430.1720639273151973No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA24830.16164764719017588No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA24510.1595643911651716No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA24470.15930398416204608No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC23640.1539005388471912No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA23230.1512313670651545No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC22450.14615343050420654No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG22260.14491649723936026No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC21230.13821101690887772No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC20870.13586735388074792No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA20760.1351512346221527No Hit
ATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATTCTT20720.13489082761902715No Hit
CATAAAAAGTTATTCTATTATTCATGCTTGTATTCTGGACTGAGATTAGT20330.1323518593385532No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA20230.13170084183073935No Hit
GAAATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATT20190.1314404348276138No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC19560.12733902452838664No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA19490.12688331227291696No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT19010.12375842823541053No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC18730.12193557921353179No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG18050.1175086601603977No Hit
ATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGT17710.11529520063383064No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT17120.11145419733772899No Hit
GGAAAAAACTACTCTCAAGTTATCAAAGGTTCTTCTGTTAAAAGTGTGAT16480.10728768528772044No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG16410.10683197303225077No Hit
GTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGTTCTTCTGT16220.10559503976740447No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA16170.10526953101349754No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT16160.10520442926271617No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT16110.10487892050880925No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG16040.10442320825333956No Hit
CTATAATTTGACTGTTAGCAGATTCTTTTAGCTACCAAGAGGACACAGTA15940.10377219074552571No Hit
ATTCTGGACTGAGATTAGTCTAGAGCTGAGAACAGGATTGGGTATTCTAA15740.10247015572989804No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA15620.10168893472052144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)