FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276071
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT67740.5308482051547289No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA61500.4819481047684651No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG55920.4382201303845946No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG52600.4122027692816465No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT47030.36855316044326686No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG45550.3569550597106274No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC40970.32106363987583764No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA40060.3139323752361742No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA32740.2565687959369032No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA32580.2553149472090503No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT27770.21762111982797194No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC27580.2161321744636466No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG26040.2040638804580623No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT25580.20045906536548516No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC25330.19849992672821498No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT24190.18956625454226295No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT23860.18698019154106627No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA23550.18455085963085127No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA21070.16511620434913105No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT20770.1627652379844068No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG20700.16221667916597118No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA19330.15148059943373057No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT19140.14999165406940523No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT18210.14270365833876014No Hit
TTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATG18000.1410579818834532No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT17860.13996086424658188No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA17610.1380017256093117No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG17490.13706133906342202No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC17270.13533729706262426No Hit
TCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTT17260.13525893151713345No Hit
GATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCC17240.13510220042615184No Hit
GACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGA16870.13220267524299195No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA16770.1314190197880839No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA16730.13110555760612067No Hit
ATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATA16680.13071372987866664No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA16370.1282843979684516No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA15810.12389592742096639No Hit
AACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAA15700.12303390642056751No Hit
ATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGT15590.12217188542016863No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA15160.11880216696406393No Hit
ACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAAC14850.11637283505384888No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA14400.11284638550676256No Hit
TTTTTATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGA14160.11096561241498318No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT13980.10955503259614865No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG13900.10892810823222218No Hit
CTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGA13670.10712570068593362No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC13480.10563675532160828No Hit
TCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAA13400.10500983095768181No Hit
CTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCA13350.1046180032302278No Hit
CCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAA13150.10305069232041163No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC12920.10124828477412307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)