FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S10_R1_A02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S10_R1_A02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240685
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT33590.27073753611915996No Hit
GTCTAATATTAACATGTAACTGATTTTGCCGTTTAGGCTTTAGCTTTTAA20600.16603731003437616No Hit
GTATTAATTCATGTGATTTATTCTAAGGAAATATTAGAAATGCTCACAAT19540.1574936426248403No Hit
CATTAATACTGAGACTTAGAAAATTAAAGGCAGCTTAAGGTTGCCAAAAT18750.15112619238565791No Hit
GTATTAAGAATATGAAAGTACCCATGTCTTCTAGGACTTACCACTACTTT15980.12879981623055006No Hit
TTCTAGGACTTACCACTACTTTAACACTTAGGGTAAATGCATACATTTTA15190.12243236599136767No Hit
CAACTACACCGTGTGTGTGAACCGCCAGCCACTTCTCCAGCTGAAGGAGG14670.11824113292253875No Hit
GTACAGAAAAGTATTAAGAATATGAAAGTACCCATGTCTTCTAGGACTTA14010.11292149095056361No Hit
TATTAGAAATGCTCACAATGGTTTAGAAGTTAGGTTAACAGTGAAATGCA13490.1087302578817347No Hit
GATTTATTCTAAGGAAATATTAGAAATGCTCACAATGGTTTAGAAGTTAG13100.10558683308011299No Hit
GAATATGAAAGTACCCATGTCTTCTAGGACTTACCACTACTTTAACACTT12940.10429722290508872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)