FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51805
Sequences flagged as poor quality0
Sequence length151
%GC64

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCG20283.9146800501882058No Hit
GTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCG20073.8741434224495706No Hit
GGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGT13902.683138693176334No Hit
CTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAA10772.0789499083100087No Hit
AAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGC10522.0306920181449666No Hit
CCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTG10101.9496187626676962No Hit
GCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCG10091.9476884470610947No Hit
AAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTC9901.9110124505356627No Hit
GGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATG9721.8762667696168325No Hit
CAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGC8581.656210790464241No Hit
GTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCC8021.548113116494547No Hit
GTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGCTATACC7661.4786217546568863No Hit
GAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGC7391.426503233278641No Hit
CCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGA7181.3859666055400057No Hit
GCACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGC6591.2720779847505068No Hit
CACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCT5881.1350255766817874No Hit
AGCGAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCC5581.077116108483737No Hit
CACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGC5481.0578129524177204No Hit
TGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCT5461.053952321204517No Hit
GAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTG5281.0192066402856867No Hit
CCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTT5160.9960428530064667No Hit
ATGGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGC5110.9863912749734582No Hit
AGCAAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCG4720.9111089663159927No Hit
GTATAGCCATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGGGACT4690.9053180194961876No Hit
CAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCAT4670.9014573882829842No Hit
GCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGC4490.8667117073641541No Hit
GTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGG4430.855129813724544No Hit
GTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGTCGGCAACACCTTG4290.8281053952321205No Hit
AGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACAGGCGGTATAGCCA4090.7894990831000868No Hit
GCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCG4040.7798475050670785No Hit
GGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTG3910.7547534021812566No Hit
GCGAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGG3830.7393108773284431No Hit
CCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATG3730.7200077212624264No Hit
CGATGGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGA3600.6949136183766046No Hit
CTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCG3550.6852620403435962No Hit
GAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTT3520.6794710935237911No Hit
AGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCC3430.662098253064376No Hit
GGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCG3350.6466557282115626No Hit
ATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGG3350.6466557282115626No Hit
CTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCG3260.6292828877521475No Hit
CCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCGT3230.6234919409323424No Hit
GCGAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCG3090.596467522439919No Hit
GCTCTTGACACAGGCGGTATAGCCATTCGAGCCCATGGTCACGCATCCAT2970.5733037351606988No Hit
AGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTG2960.5713734195540972No Hit
GCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCC2900.559791525914487No Hit
GATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGC2720.5250458449956568No Hit
GTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTC2650.5115336357494451No Hit
GCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCG2640.5096033201428434No Hit
GGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTC2540.49030016407682653No Hit
AAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCC2430.4690666924042081No Hit
GCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCA2430.4690666924042081No Hit
GGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGC2430.4690666924042081No Hit
GCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCA2360.45555448315799635No Hit
CACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGC2260.43625132709197956No Hit
GAGCGAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTC2140.4130875398127594No Hit
CACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGC2050.3957146993533443No Hit
ACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTG2000.3860631213203359No Hit
GTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAG1900.3667599652543191No Hit
GCAAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGG1850.3571083872213107No Hit
GCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTG1810.34938712479490397No Hit
AAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGA1800.3474568091883023No Hit
GCCACAGCCGGCCCGGCTCTTGACACAGGCGGTATAGCCATTCGAGCCCA1790.34552649358170057No Hit
GAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGG1760.33973554676189555No Hit
GCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCA1750.3378052311552939No Hit
GCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGA1620.31271112826947206No Hit
GTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGG1540.2972686034166586No Hit
GAGCAAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTC1510.2914776565968536No Hit
GCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGAT1510.2914776565968536No Hit
ACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGCT1480.2856867097770486No Hit
CGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCG1460.2818260785638452No Hit
GCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAA1460.2818260785638452No Hit
GCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCG1450.2798957629572435No Hit
AACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGT1440.2779654473506418No Hit
CCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGAGG1360.2625229224978284No Hit
CCACGATGGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCA1350.2605926068912267No Hit
GTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGTCGGCAACACCT1340.25866229128462503No Hit
CTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGT1320.2548016600714217No Hit
GTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACAG1280.24708039764501497No Hit
AGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGCTATAC1270.24515008203841326No Hit
AGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACAC1270.24515008203841326No Hit
CCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCG1220.2354985040054049No Hit
ACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCT1210.23356818839880322No Hit
CCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGT1200.23163787279220152No Hit
GGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGG1190.22970755718559982No Hit
CGGTATAGCCATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGGGA1180.22777724157899817No Hit
CTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGG1160.2239166103657948No Hit
GCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCG1120.21619534793938808No Hit
GGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCG1110.21426503233278638No Hit
AGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCG1100.21233471672618473No Hit
GAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCC1100.21233471672618473No Hit
GAGCGAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGC1100.21233471672618473No Hit
GCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACAGGCGGT1100.21233471672618473No Hit
ATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGTCG1070.2065437699063797No Hit
GAGCAAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGC1060.204613454299778No Hit
GCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTT1060.204613454299778No Hit
TGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGC1020.19689219187337131No Hit
GGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTC1020.19689219187337131No Hit
ACCTTGTTCATCGGCTACGTGCTGCGCGAACTTCTGGGGCGACGTCAGCA970.1872406138403629No Hit
TTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACA960.18531029823376122No Hit
CGAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGT960.18531029823376122No Hit
CGAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGC910.17565872020075282No Hit
GACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTG900.17372840459415115No Hit
AACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTG880.16986777338094777No Hit
GCTACGTGCTGCGCGAACTTCTGGGGCGACGTCAGCACTAAAGGGCGCGG870.1679374577743461No Hit
AAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGT840.16214651095454105No Hit
GGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCT820.1582858797413377No Hit
GGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATG790.15249493292153268No Hit
CATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGG790.15249493292153268No Hit
AGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCC790.15249493292153268No Hit
CGCCACGATGGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGT790.15249493292153268No Hit
GCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCC790.15249493292153268No Hit
GGCTGTGGCGGCGCTACCTCGTCGGCAACACCTTGTTCATCGGCTACGTG750.14477367049512596No Hit
CCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGTCGGCAACA740.1428433548885243No Hit
CGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCT720.1389827236753209No Hit
CTTGACACAGGCGGTATAGCCATTCGAGCCCATGGTCACGCATCCATCGT720.1389827236753209No Hit
TCTTGACACAGGCGGTATAGCCATTCGAGCCCATGGTCACGCATCCATCG700.13512209246211757No Hit
GAGCCGGGCCGGCTGTGGCGGCGCTACCTCGTCGGCAACACCTTGTTCAT690.13319177685551586No Hit
GATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTC690.13319177685551586No Hit
GTCACGCATCCATCGTGGCGCCTGGGACTTGGTGCCAGCGATCAGCGCAA680.1312614612489142No Hit
CGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGAT670.12933114564231252No Hit
CAGCCGGCCCGGCTCTTGACACAGGCGGTATAGCCATTCGAGCCCATGGT670.12933114564231252No Hit
GCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCG660.12740083003571084No Hit
CCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGCTA660.12740083003571084No Hit
CTGGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAA650.12547051442910914No Hit
CGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGA650.12547051442910914No Hit
TAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCC640.12354019882250748No Hit
CGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCG640.12354019882250748No Hit
AGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCG640.12354019882250748No Hit
GCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCC630.1216098832159058No Hit
CCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGG630.1216098832159058No Hit
CATCCATCGTGGCGCCTGGGACTTGGTGCCAGCGATCAGCGCAAAGGCCA600.11581893639610076No Hit
GGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACA600.11581893639610076No Hit
CCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAG600.11581893639610076No Hit
CCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGC580.1119583051828974No Hit
GGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGT580.1119583051828974No Hit
AAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCG570.11002798957629573No Hit
GGCGGTATAGCCATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGG570.11002798957629573No Hit
ATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGGGACTTGGTGCCA570.11002798957629573No Hit
ACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCC570.11002798957629573No Hit
ACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCG550.10616735836309237No Hit
CGCATCCATCGTGGCGCCTGGGACTTGGTGCCAGCGATCAGCGCAAAGGC540.10423704275649068No Hit
GTTCATCGGCTACGTGCTGCGCGAACTTCTGGGGCGACGTCAGCACTAAA530.102306727149889No Hit
GCGCTACCTCGTCGGCAACACCTTGTTCATCGGCTACGTGCTGCGCGAAC530.102306727149889No Hit
CGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCA530.102306727149889No Hit
GCAAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCG520.10037641154328732No Hit
TCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTC520.10037641154328732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)