FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_H07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_H07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903284
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT79110.4156500028372013No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA72340.3800799039975117No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT60040.3154547613493309No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA57210.3005857244636113No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG50740.26659184861534063No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG46280.24315866680957757No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA45600.23958589469569436No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC44760.23517247031972108No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA43830.23028617904632204No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG40800.2143663268329897No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA40080.210583391653584No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA39700.2085868425311199No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG34170.1795317987226289No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC33010.17343706982247525No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG30680.16119507125578736No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC30140.15835786987123307No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA27600.14501251521055186No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC26860.1411244984983849No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG26560.13954827550696586No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA26390.13865508247849506No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG25460.13376879120509602No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA25150.13214002744729636No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA25090.13182478284901256No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT24420.12830455150151002No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC23600.12399620865829798No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG23570.12383858635915607No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC23170.12173695570393069No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA23140.12157933340478878No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT23030.12100138497460179No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT22840.12000311041336974No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA22800.11979294734784718No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT22390.11763877592624117No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC21880.1149591968408288No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT21740.11422362611149993No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC21560.11327789231664849No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA21380.11233215852179707No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA20900.1098102017355266No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT20520.10781365261306247No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC20210.1061848888552628No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA20170.10597472578974027No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA19820.10413579896641804No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)