FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_G10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_G10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968246
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGTAGGTAAACCTCTAGCTTTAATCTTTTGCCATCTCTGATCTC57270.2909697263451825No Hit
GTCTTAAGTACTATTGCTTTATAGTAAGTTTTGAAATCAGGAAGTGGGAA51260.2604349253091331No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT47100.23929935587319878No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG46120.2343203034580027No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT37480.19042335155260065No Hit
ATATTAGAGAAGATCTAAATAATTGGAAAAAACATCCTGTGTTCATGGAC37100.18849269857527973No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA34450.17502893439133116No Hit
ACTTAAGACAGTGTGCAAGTGTCACAAGGATAAACAAACAGATTAACAGC32480.1650200229036411No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA23630.12005613119498275No Hit
GATCTAAATAATTGGAAAAAACATCCTGTGTTCATGGACTGGAAGACAAC22850.11609321192574507No Hit
CTTATAGCCTGATACTTTGTTGAAGAAATGCAGTAATGCGTTATACACTA22110.11233351928569904No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)