FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088757
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT88900.4256119787988742No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA57820.2768153499904489No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA54790.26230911494252324No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG54240.2596759699668272No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC46460.22242893740152636No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA45620.21840740689319055No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA41170.19710287027164958No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC39270.18800655126469953No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG33510.16043034206468249No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT30880.1478391215445358No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG28550.13668416192022337No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG28380.1358702807459173No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG25880.1239014399472988No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA25850.12375781385771537No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT24720.11834789781673982No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA24220.11595412965701611No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA23860.11423061658201504No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA23020.11020908607367921No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT22530.10786319327715001No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC22060.10561305120700973No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA21100.1010170163403402No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC21090.10096914097714572No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)