FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919953
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT230541.200758560235589No Hit
TCTTTATCTCGCTCACAGGAAAGGTGTCGCCCCTCTGGCAGGTGGGCAGG82460.4294896802161303No Hit
TCATAAACCGGTGGCACTTCCCACAGCGGGACAGGGGCTTGCCCGTGGCC78700.4099058674873812No Hit
CCCTAAACACTGTGGAGATGCTGCGAGTAGCAAGCTCTTCTCTGGGCATG75240.39188459300826634No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA70720.3683423500471105No Hit
GTATGAGTACATCACCAGACACTTCATCGCCACGGTGAGCTACGACTGCA68300.35573787483339436No Hit
GTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCATAAACCGGTGGCACT67770.3529773905923739No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG64990.33849786947909666No Hit
ATCTTGTAGTAGCCATGCACCAGGACGATGCCCAGGTTGGTGGGCTTGAG60750.31641399555093275No Hit
GACGAGTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGAGTCTAGT60670.31599731868436365No Hit
GTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCATAAACC57690.3004761054046636No Hit
GCCCAGGACCTGGCGGTAGTCAGCCTTGCCCTGGGCGATCAGGTTTAGCT53120.27667343940190203No Hit
TCCCACAGCGGGACAGGGGCTTGCCCGTGGCCGCCAGGGGCGAAAAAGAC52760.2747983935023409No Hit
GGCCTAGCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGGGATCC52710.27453797046073525No Hit
TTCATGATCCGCTCCATGTTGGCTGTCCAGCCATAGGTGCTAGTCACAAT51480.26813156363723484No Hit
TCACCAGACACTTCATCGCCACGGTGAGCTACGACTGCAAGTACCTCCAG50920.265214825571251No Hit
AGATAAAGATGCTGGAGAAACAGACGAGCCCGCCCGACTACCTGACTGAG49610.25839174188118147No Hit
GTCCCAATGCCATGCTTCTCCATGAGTGTGATGAGCTCAGCCTCAGTCAG49580.25823548805621804No Hit
TCCCTGTGCACATCAACAACATCTGTCAACGCAACTATGTCACTGTGGAG46280.24104756731024146No Hit
CCTCCAGAGGCACGCTCTGCCAGGGCATGATCTCCGTGAAGCCTGGAGAG43450.22630762315535852No Hit
CACCAGGACGATGCCCAGGTTGGTGGGCTTGAGCCGGCGCCCACTCTCCA42650.2221408544896672No Hit
ATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGTTT42380.22073457006499636No Hit
GCCTTGCCCTGGGCGATCAGGTTTAGCTGCTTCTCGACTGCACTGCGGAT41640.21688030904923195No Hit
GAATTAGGGGGCGAGGCATGGCGGCTGTATGAGTACATCACCAGACACTT41140.21427607863317488No Hit
TCTCTGGGCATGGGGCCTCAGCATGCCATGCAGACCGCCGAGCGGCTCTA40580.21135934056719097No Hit
CAGTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCATAAA40260.20969263310091443No Hit
CTACAAGATTGATGCAGAGCTGGTGCTCCCCACCATCCGCAGTGCAGTCG39840.20750507955142652No Hit
GTAAAGCGCTTGTTAGCAGAAGGTATCAACCGGCCACGGAAGGGCCACGA39800.20729674111814195No Hit
GTCCTGGTGCATGGCTACTACAAGATTGATGCAGAGCTGGTGCTCCCCAC39340.20490084913536943No Hit
CTCATGGAGAAGCATGGCATTGGGACAGACGCCAGCATCCCTGTGCACAT37520.19542145042092174No Hit
GTACTGGGCTGACACAGTAAAGCGCTTGTTAGCAGAAGGTATCAACCGGC37410.1948485197293892No Hit
GCTCTACACACAAGGCTACATCAGCTACCCGCGGACCGAGACCACCCACT36720.19125468175523047No Hit
CAGGAAAGGTGTCGCCCCTCTGGCAGGTGGGCAGGCTCTCCTCCAGAGGC36670.19099425871362477No Hit
CTCCAGGCTTCACGGAGATCATGCCCTGGCAGAGCGTGCCTCTGGAGGAG36240.18875462055581568No Hit
GTACATCACCAGACACTTCATCGCCACGGTGAGCTACGACTGCAAGTACC35930.18713999769786033No Hit
GTAGTGAAACTTCCTCTTGAAGACATCCAGTGTGTGGCCCAGGACCTGGC35180.1832336520737747No Hit
AGTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCATAAAC35150.1830773982488113No Hit
GTCACAGTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCA34670.18057733704939652No Hit
TCATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATC34430.17932730644968914No Hit
GTAGTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAA33820.17615014534209952No Hit
TCTCGTCACAGTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTAC33780.17594180690881495No Hit
CCCATGAGGTCTGCCACTGAGGCCGAATTAGGGGGCGAGGCATGGCGGCT33440.17417093022589614No Hit
GTGGAGAGTGGGCGCCGGCTCAAGCCCACCAACCTGGGCATCGTCCTGGT32650.170056246168526No Hit
CTCCATGAGTGTGATGAGCTCAGCCTCAGTCAGGTAGTCGGGCGGGCTCG32630.16995207695188372No Hit
GTACTCATACAGCCGCCATGCCTCGCCCCCTAATTCGGCCTCAGTGGCAG32380.1686499617438552No Hit
AGCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGGGATCCTCCAG32180.16760826957743236No Hit
GCACAGGGATGCTGGCGTCTGTCCCAATGCCATGCTTCTCCATGAGTGTG31490.16401443160327364No Hit
GTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCATGTTGGCTGTCCAGC31150.16224355492035483No Hit
GTGACATAGTTGCGTTGACAGATGTTGTTGATGTGCACAGGGATGCTGGC31050.1617227088371434No Hit
GTCTGGGGATCCTCCAGTGAGAAGCCAGAAGAGAGCAGAGCTGTTTCAAA30610.1594309860710132No Hit
CTGTGGAGATGCTGCGAGTAGCAAGCTCTTCTCTGGGCATGGGGCCTCAG30110.15682675565495613No Hit
CCTTTCCTGTGAGCGAGATAAAGATGCTGGAGAAACAGACGAGCCCGCCC29550.15391001758897224No Hit
GCATTGGGACAGACGCCAGCATCCCTGTGCACATCAACAACATCTGTCAA29540.1538579329806511No Hit
GCCCAGGCCTAGCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGG29460.15344125611408196No Hit
CCATTACCCCCATGAGGTCTGCCACTGAGGCCGAATTAGGGGGCGAGGCA29440.1533370868974397No Hit
GGAAAGGTGTCGCCCCTCTGGCAGGTGGGCAGGCTCTCCTCCAGAGGCAC29360.15292041003087056No Hit
GGTTTAGCTGCTTCTCGACTGCACTGCGGATGGTGGGGAGCACCAGCTCT29310.15265998698926483No Hit
TTGTTGATGTGCACAGGGATGCTGGCGTCTGTCCCAATGCCATGCTTCTC29230.1522433101226957No Hit
CATCACACTCATGGAGAAGCATGGCATTGGGACAGACGCCAGCATCCCTG29130.1517224640394843No Hit
GTCTAGTCTCTGTTACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGAG28560.14875364136517924No Hit
GCCTGAGGATGAAGAGGAGAAAAAGAAAATGGAGGAGAGCAAGGCCAAGT28560.14875364136517924No Hit
CATCAACAACATCTGTCAACGCAACTATGTCACTGTGGAGAGTGGGCGCC28440.14812862606532556No Hit
GCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGGGATCCTCCAGT28220.14698276468226046No Hit
CTTCACCTGCACAGGCAAAACCGTCATCTCTCCAGGCTTCACGGAGATCA28190.14682651085729703No Hit
GGCAAAACCGTCATCTCTCCAGGCTTCACGGAGATCATGCCCTGGCAGAG28160.1466702570323336No Hit
GAATTGGGTCCGAGCACTTCACCTGCACAGGCAAAACCGTCATCTCTCCA28030.14599315712415878No Hit
CACCTGCACAGGCAAAACCGTCATCTCTCCAGGCTTCACGGAGATCATGC28000.14583690329919535No Hit
GTGGAGATGCTGCGAGTAGCAAGCTCTTCTCTGGGCATGGGGCCTCAGCA28000.14583690329919535No Hit
GGATGTACTTCATAAACCGGTGGCACTTCCCACAGCGGGACAGGGGCTTG27880.14521188799934165No Hit
CTCCACAGTGACATAGTTGCGTTGACAGATGTTGTTGATGTGCACAGGGA27830.14495146495773595No Hit
CTGTTACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGAGGAGAAAAAG27730.14443061887452455No Hit
GTCCTTGACAGCTTTGTCGTTCTTGTCCGCCTCTGCCTTCTGCCGCAGCG27530.14338892670810172No Hit
CTCCAGAGCACCATCGCCTTCAGAATTGGGTCCGAGCACTTCACCTGCAC27320.14229514993335776No Hit
CATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCC27170.14151388080854063No Hit
CCATCAGCTCATCCATGCCAGCGATAGAGTCCACGAAGTAGTGAAACTTC26870.1399513425589064No Hit
CTATGTCACTGTGGAGAGTGGGCGCCGGCTCAAGCCCACCAACCTGGGCA26750.1393263272590527No Hit
CATCTAGGTCTAGCCTCAAGGCTGGGTACCTCCCAAATGGCCAAGGAAGG26600.1385450581342356No Hit
CTTCATCGCCACGGTGAGCTACGACTGCAAGTACCTCCAGAGCACCATCG26530.1381804658759876No Hit
ATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCATGTT26520.13812838126766647No Hit
TTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCATGTTG26320.13708668910124364No Hit
GTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGAGTCTAGTCTCTG26280.13687835066795906No Hit
CAGTAAAGCGCTTGTTAGCAGAAGGTATCAACCGGCCACGGAAGGGCCAC26270.1368262660596379No Hit
GGAAAGAGAAGGCCAAGCAGCGGCCCCTCGCCCTAAACACTGTGGAGATG26240.13667001223467448No Hit
GTCTGGTGATGTACTCATACAGCCGCCATGCCTCGCCCCCTAATTCGGCC26180.13635750458474766No Hit
GGACAGGGGCTTGCCCGTGGCCGCCAGGGGCGAAAAAGACACCTCCATCA25860.13469079711847112No Hit
GCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGA25750.13411786642693857No Hit
GATCATGCCCTGGCAGAGCGTGCCTCTGGAGGAGAGCCTGCCCACCTGCC25710.13390952799365402No Hit
GTGCAGGTGAAGTGCTCGGACCCAATTCTGAAGGCGATGGTGCTCTGGAG25620.13344076651876374No Hit
AGCAGGAACAGCAGCACTGGGTTCCTCTGCTGTCACTTCATCTTCATCAA25550.13307617426051574No Hit
AGTGTGATGAGCTCAGCCTCAGTCAGGTAGTCGGGCGGGCTCGTCTGTTT25450.13255532817730434No Hit
CCATGAGTGTGATGAGCTCAGCCTCAGTCAGGTAGTCGGGCGGGCTCGTC25430.13245115896066204No Hit
CCACAATGGGGTGGTCAGGATTGATCTCCAGGTGCTTCTTGGCCATCATA25380.13219073591905636No Hit
CCAGAGCACCATCGCCTTCAGAATTGGGTCCGAGCACTTCACCTGCACAG25230.13140946679423923No Hit
GGCAGAGCGTGCCTCTGGAGGAGAGCCTGCCCACCTGCCAGAGGGGCGAC25140.13094070531934895No Hit
TTTGTGGAGCGAGTGCGAAAGCGGGGCTTCGAGGTAGTATATATGACAGA24910.1297427593279627No Hit
GTCTTCACCCTGCTGCATTGTGACTAGCACCTATGGCTGGACAGCCAACA24800.12916982863643017No Hit
GACTGAGGCTGAGCTCATCACACTCATGGAGAAGCATGGCATTGGGACAG24520.1277114596034382No Hit
GCCCTGGGCGATCAGGTTTAGCTGCTTCTCGACTGCACTGCGGATGGTGG24510.12765937499511706No Hit
CATCCAGTGTGTGGCCCAGGACCTGGCGGTAGTCAGCCTTGCCCTGGGCG24450.1273468673451902No Hit
GCCCTAAACACTGTGGAGATGCTGCGAGTAGCAAGCTCTTCTCTGGGCAT24010.12505514457906No Hit
GTTTCAAACAGCAGCACCACCAGGTCCTTGACAGCTTTGTCGTTCTTGTC23960.1247947215374543No Hit
CCCTAATTCGGCCTCAGTGGCAGACCTCATGGGGGTAATGGGGGGATGGT23910.1245342984958486No Hit
GTCCACGAAGTAGTGAAACTTCCTCTTGAAGACATCCAGTGTGTGGCCCA23860.1242738754542429No Hit
GCTCTGCATCAATCTTGTAGTAGCCATGCACCAGGACGATGCCCAGGTTG23790.12390928319599491No Hit
GGTCAGGATTGATCTCCAGGTGCTTCTTGGCCATCATATAGCCCATTGTG23400.1218779834714704No Hit
GGTATCAACCGGCCACGGAAGGGCCACGACGCTGGTGACCATCCCCCCAT23260.12114879895497442No Hit
ATCTTCATCAATGCCCAGGCCTAGCTTAATCATGCGGTAGATGCGGTTGG23240.12104462973833213No Hit
GCCATGCAGACCGCCGAGCGGCTCTACACACAAGGCTACATCAGCTACCC22930.11943000688037675No Hit
GCATCAATCTTGTAGTAGCCATGCACCAGGACGATGCCCAGGTTGGTGGG22910.11932583766373447No Hit
AGGTAGTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTC22810.11880499158052307No Hit
CTGCCAGGGCATGATCTCCGTGAAGCCTGGAGAGATGACGGTTTTGCCTG22770.11859665314723851No Hit
ATCTCTAACAGGCTAGTGTCTTCACCCTGCTGCATTGTGACTAGCACCTA22610.11776329941410024No Hit
GTTAGAGATGGTCACCTTCTCAACCTTCTTATCCAAGATTTCTTTCATGA22520.11729453793920996No Hit
GCCATGCACCAGGACGATGCCCAGGTTGGTGGGCTTGAGCCGGCGCCCAC22480.1170861995059254No Hit
GAAGAGAGCAGAGCTGTTTCAAACAGCAGCACCACCAGGTCCTTGACAGC22310.11620076116446601No Hit
GGCCTGGATGTACTTCATAAACCGGTGGCACTTCCCACAGCGGGACAGGG22190.1155757458646123No Hit
CACTCATGGAGAAGCATGGCATTGGGACAGACGCCAGCATCCCTGTGCAC22140.1153153228230066No Hit
GCACTGGGTTCCTCTGCTGTCACTTCATCTTCATCAATGCCCAGGCCTAG22090.1150548997814009No Hit
CCACAGTGACATAGTTGCGTTGACAGATGTTGTTGATGTGCACAGGGATG22080.11500281517307977No Hit
CCATGTTGGCTGTCCAGCCATAGGTGCTAGTCACAATGCAGCAGGGTGAA21950.11432571526490491No Hit
GCCATAGGTGCTAGTCACAATGCAGCAGGGTGAAGACACTAGCCTGTTAG21870.11390903839833579No Hit
AGTCTAGTCTCTGTTACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGA21860.11385695379001466No Hit
AGGCTACATCAGCTACCCGCGGACCGAGACCACCCACTATCCCGAGAACT21740.11323193849016096No Hit
GGGCTATATGATGGCCAAGAAGCACCTGGAGATCAATCCTGACCACCCCA21670.11286734623191298No Hit
CATGGAGAAGCATGGCATTGGGACAGACGCCAGCATCCCTGTGCACATCA21590.11245066936534384No Hit
CATTACCCCCATGAGGTCTGCCACTGAGGCCGAATTAGGGGGCGAGGCAT21240.1106277080741039No Hit
GGATTGATCTCCAGGTGCTTCTTGGCCATCATATAGCCCATTGTGGAGTT21060.10969018512432337No Hit
ACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGAGGAGAAAAAGAAAAT20850.10859640834957938No Hit
GACCGAGACCACCCACTATCCCGAGAACTTCGACCTGAAGGGCCCTCTGC20820.10844015452461597No Hit
GCGATAGAGTCCACGAAGTAGTGAAACTTCCTCTTGAAGACATCCAGTGT20700.10781513922476227No Hit
CCCGTACTGGGCTGACACAGTAAAGCGCTTGTTAGCAGAAGGTATCAACC20660.1076068007914777No Hit
GTAGATGCGGTTGGAGTGAGTCTGGGGATCCTCCAGTGAGAAGCCAGAAG20570.10713803931658744No Hit
ACCCCCATGAGGTCTGCCACTGAGGCCGAATTAGGGGGCGAGGCATGGCG20540.106981785491624No Hit
ACTCTCCACAGTGACATAGTTGCGTTGACAGATGTTGTTGATGTGCACAG20440.1064609394084126No Hit
TACCTATTCTGTTAAGAAAAAGTAGGGCATGAAAATCTTTATAGCAAACT20440.1064609394084126No Hit
AGCGAAAGCAGGTCTCGTCACAGTGTGAGCAGTGCAGGCGGCTCGGCTTG20370.10609634715016461No Hit
GTGCACAGGGATGCTGGCGTCTGTCCCAATGCCATGCTTCTCCATGAGTG20330.10588800871688005No Hit
CCCTGGCAGAGCGTGCCTCTGGAGGAGAGCCTGCCCACCTGCCAGAGGGG20280.10562758567527433No Hit
ACTGTGGAGAGTGGGCGCCGGCTCAAGCCCACCAACCTGGGCATCGTCCT20010.10422130125060353No Hit
GTCTGCCACTGAGGCCGAATTAGGGGGCGAGGCATGGCGGCTGTATGAGT19970.10401296281731895No Hit
CTCTGGAGGAGAGCCTGCCCACCTGCCAGAGGGGCGACACCTTTCCTGTG19830.10328377830082298No Hit
ATATATTACATGGTATGCAATTCTGTTCTTTCTTCACATTCATGAAACAA19800.10312752447585957No Hit
GTGTGGCCCAGGACCTGGCGGTAGTCAGCCTTGCCCTGGGCGATCAGGTT19790.10307543986753842No Hit
ATATTACATGGTATGCAATTCTGTTCTTTCTTCACATTCATGAAACAAAA19590.10203374770111559No Hit
ATCCCGAGAACTTCGACCTGAAGGGCCCTCTGCGACAGCAGGCCAACCAC19530.10172124005118875No Hit
GTGTGTGGCCCAGGACCTGGCGGTAGTCAGCCTTGCCCTGGGCGATCAGG19420.1011483093596562No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)