FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_F09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_F09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687394
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT90730.537693034347639No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA62880.3726456298884552No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG54160.3209683097130842No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA52840.3131455961085556No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA50280.2979742727543182No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA46710.2768173882329794No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA44840.2657352106265638No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG41810.24777852712525944No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC39340.23314057060769447No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC37610.22288807474721375No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG33630.19930140796992285No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA32160.19058974963760686No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA31370.18590797407126017No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT30740.18217440621455333No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT30180.17885567923081389No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC29940.17743336766635415No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG29700.1760110561018944No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT28800.17067738773517033No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG28630.169669917043678No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA27410.16243983325767425No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA27290.1617286774754444No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA26150.15497269754426055No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT25620.1518317595060786No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT25490.15106134074199623No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG25160.1491056623408641No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA24790.14691293201232197No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA23630.14003842611743314No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC23530.13944579629890824No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC23320.13820127368000598No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA22880.13559370247849642No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT22750.13482328371441407No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA22700.1345269688051516No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA22380.13263055338587196No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG22120.1310897158577072No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA21460.1271783590554429No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA21190.12557825854542568No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT20970.12427447294467091No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC20920.12397815803540843No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC20350.12060016806981654No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG20350.12060016806981654No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA20340.12054090508796406No Hit
CAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTA19300.1143775549753051No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT18740.11105882799156569No Hit
GTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAA18660.11058472413674578No Hit
GTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCT18650.11052546115489327No Hit
CTTCTAATTGTGGTAAAATACAATAACACAAAACTCACACCTTAATCATT18650.11052546115489327No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT18200.10785862697153124No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT17970.10649557838892398No Hit
GGTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATC17150.10163601387701983No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG16890.10009517634885509No Hit
GTAAAATACAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACG16880.10003591336700261No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)