FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440790
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAATAGAAGGTAGAAAGTCAATTAGAGACGATTACAAACTAACTGAC22260.15449857369915115No Hit
TTCCTATACAGATGGCTCTAATTTGGGAAGTACACTGACTTTTTGACTTG19250.1336072571297691No Hit
ATATTAAGGATCATCCTGTCTATTTCTGCTAATACCATCCCCTTAGCAGT16970.11778260537621721No Hit
ATGTAGTACAAAGCTGACAGTGAACACTTTTACAAAATCACTGAAAAGAT16140.11202187688698562No Hit
GTATAGGAAATCTCCATTTTACAGGAGCGTAGTGCATCTGTTGTTTGTTT15590.10820452668327792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)