FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837278
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAA507156.057127978998612No Hit
ATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAA393604.7009475944668315No Hit
GAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCAT231702.7673007053810084No Hit
CTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTT222632.6589734831203016No Hit
ACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGT198502.370777686742038No Hit
AAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGA173042.0666970826893816No Hit
GTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCA144581.7267860853862158No Hit
AGCGAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGG125071.4937690946137365No Hit
GAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGG112791.3471033515749846No Hit
TCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCA111821.3355181910906533No Hit
GGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAA106661.273889914699777No Hit
ACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATC105351.2582439763137214No Hit
CCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAG99451.1877775362543863No Hit
GTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGT93211.1132503182933267No Hit
GTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTAT92331.1027400696065106No Hit
CAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTT84501.009222743222681No Hit
ATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGA77170.9216771490472699No Hit
ATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAATG75370.9001789130969643No Hit
GACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCAT74850.8939683116002092No Hit
GCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCT70570.8428502838961491No Hit
CTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCA70000.8360425091785525No Hit
AGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGT69360.8283986919517771No Hit
ATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTA67680.8083336717314917No Hit
TAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAA67390.8048700670506093No Hit
CTCACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGG67050.8008092891488847No Hit
AAACAAAGCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTT66980.7999732466397063No Hit
GCCCTAAACAAAGCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTT66000.7882686515112066No Hit
ACATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAATGGCTTTTAAAGTTA62310.74419726781308No Hit
AAGCAGGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTC59870.715055214635999No Hit
ACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCT57850.6909294165139894No Hit
GGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCC57030.6811357756921834No Hit
GGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGG56790.6782693442321427No Hit
TGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGC56130.6703866577170307No Hit
AAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTT53470.6386170423682457No Hit
GCTTCTCACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACAT53410.6379004345032355No Hit
CTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGG53110.6343173951781845No Hit
CTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGACGATTTCTATGTTGG52780.6303760519206285No Hit
GCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTTTGCCAGG52480.6267930125955776No Hit
GTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGAC49540.5916792272100784No Hit
CTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAG49290.5886933611058692No Hit
CCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTT47740.5701809912597727No Hit
CCCTAAACAAAGCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTTT46360.5536990103645384No Hit
CATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAATGGCTTTTAAAGTTAC46010.5495187978186457No Hit
GGGAAATAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTG45820.5472495395794468No Hit
GTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAG45340.5415166766593652No Hit
TCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTCTCCTTATC44940.5367392908926306No Hit
ACCCAGGCGCCCTAAACAAAGCATTATTTTAGGGACCAACATAGAAATCG44300.5290954736658553No Hit
ACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAG43440.518824094267376No Hit
GGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGA43310.5172714438931871No Hit
TCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGC43290.5170325746048505No Hit
CACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAA41430.4948177307895346No Hit
GAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCC38620.46125659577822425No Hit
CCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCT37200.4442968763063164No Hit
GCGAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGC36910.4408332716254339No Hit
AGCGAAAGCAGGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAA36640.437608536232888No Hit
TTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATA36400.4347421047728473No Hit
GTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGACGATTTC36070.43080076151529123No Hit
ACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAA36020.4302035882944494No Hit
GGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTCTCCTT35790.427456591478577No Hit
TGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAG35690.42626224503689336No Hit
ATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTG34180.40822761376747035No Hit
GGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCA32910.39305941395808797No Hit
TTATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAA32910.39305941395808797No Hit
AAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATG32690.390431851786384No Hit
GCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAA32410.3870876817496698No Hit
GCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTG31570.3770551716395271No Hit
GCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTA31320.37406930553531803No Hit
ATTTCTATGTTGGTCCCTAAAATAATGCTTTGTTTAGGGCGCCTGGGTGG31290.37371100160281295No Hit
ATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGAC30560.3649922725785223No Hit
GTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACTTTAAA30130.3598565828792826No Hit
GGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAG30030.358662236437599No Hit
CAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAG28450.33979156265899735No Hit
GCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAGG27770.33167000685554854No Hit
GATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGA27220.32510110142628856No Hit
CTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAGGG27210.32498166678212015No Hit
AACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCC26550.3170989802670081No Hit
TTTCTATGTTGGTCCCTAAAATAATGCTTTGTTTAGGGCGCCTGGGTGGC26350.31471028738364076No Hit
GACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAGGGACCAACA26060.31124668270275824No Hit
CACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCT25850.3087385551752226No Hit
GCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATG25800.30814138195438073No Hit
GATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTT25550.30515551585017164No Hit
GACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCC24510.29273431285666174No Hit
CCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGG23600.2818657602373405No Hit
GGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTA23480.2804325445073202No Hit
GTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAA23370.27911876342146813No Hit
GCGAAAGCAGGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAAC23250.27768554769144776No Hit
ATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTCTCCTTAT23220.2773272437589427No Hit
GAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAG22850.2729081619247132No Hit
GTTTAGGGCGCCTGGGTGGCTCAGTCAGCTAAGCATCTGCCTTTAGTGCC22690.2709972076180193No Hit
CTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCC21360.25511239994362683No Hit
AGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGC21150.2526042724160912No Hit
GGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAAT20970.2504544488210606No Hit
ATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTT20620.24627423627516784No Hit
TATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGG20260.24197458908510674No Hit
CCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCAC19990.2387498536925609No Hit
AAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAG19510.23301699077247937No Hit
CCCTAAAATAATGCTTTGTTTAGGGCGCCTGGGTGGCTCAGTCAGCTAAG19230.2296728207357652No Hit
GGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAA18660.2228650460181684No Hit
ACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCA18600.2221484381531582No Hit
ATAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAA18590.22202900350898985No Hit
CTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATT18550.22155126493231642No Hit
GGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGA18110.21629614058890834No Hit
GAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAGGGACCAACATAGA17820.21283253590802576No Hit
GGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAAC16890.20172511400036786No Hit
CAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGC16720.19969472504950567No Hit
CCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTTAGCTG16420.19611168572445473No Hit
CTGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCAT16270.19432016606192926No Hit
GCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACA16150.1928869503319089No Hit
TAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACT15850.18930391100685795No Hit
ACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGT15830.1890650417185212No Hit
TGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACT15590.18619861025848045No Hit
AAATAATGCTTTGTTTAGGGCGCCTGGGTGGCTCAGTCAGCTAAGCATCT15500.18512369846096516No Hit
CATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTT14960.1786742276758735No Hit
GAAATAGTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGC14820.17700214265751638No Hit
GCCATTGCCTGGCAAAAGAAAAGCAGACGATTTCTATGTTGGTCCCTAAA14360.1715081490257716No Hit
CCCAGGCGCCCTAAACAAAGCATTATTTTAGGGACCAACATAGAAATCGT13950.16661132861486866No Hit
GAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCTG13900.16601415539402684No Hit
ACCCAGGCACTAAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCC13880.16577528610569012No Hit
TACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAATAGTGGGAATGAT13760.16434207037566972No Hit
TCTCACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGG13500.16123676962729225No Hit
GAGCGAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTG13360.15956468460893514No Hit
TAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATGT13190.15753429565807295No Hit
TGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATTATTTTAG12930.15442899490969547No Hit
GCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGG12890.153951256333022No Hit
ATGTTCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACTTTA12310.14702404697125687No Hit
ATAAACATTCTCCTTATCTTTACATAGGGCTACTTATTTTGTGTTAAATG12100.14451591944372122No Hit
GGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTA11800.14093288011867025No Hit
CTTTTAAAGTTACTGACAATGATCATTCCCACTATTTCCCAAAAGAAAGA11690.13961909903281827No Hit
GTGGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGA11610.13866362187947134No Hit
GCCTGCCATGGGTCTCCATCCCATGACCCAGGCACTAAAGGCAGATGCTT11580.13830531794696624No Hit
CGAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTGGCT11420.1363943636402724No Hit
AGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCATT11230.13412510540107347No Hit
AAGCATTATTTTAGGGACCAACATAGAAATCGTCTGCTTTTCTTTTGCCA10840.12946715427850725No Hit
CAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGCTAC10540.12588411495345633No Hit
AGCAGGATCGATGAGCTTCTCACAAAGTGATACTCAATTATAAACATTCT10530.12576468030928795No Hit
CCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCCCATGACCCAGGCA10360.12373429135842576No Hit
CAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATGGGTCTCCATCC10270.12265937956091047No Hit
CCAACATAGAAATCGTCTGCTTTTCTTTTGCCAGGCAATGGCTTTTAAAG10180.1215844677633952No Hit
CTTTACATAGGGCTACTTATTTTGTGTTAAATGTTCTTTCTTTTGGGAAA9980.11919577488002789No Hit
TCTTTCTTTTGGGAAATAGTGGGAATGATCATTGTCAGTAACTTTAAAAG9670.11549330091080859No Hit
GGGAATGATCATTGTCAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAA9030.10784948368403327No Hit
TCACAAAGTGATACTCAATTATAAACATTCTCCTTATCTTTACATAGGGC8620.10295266327313031No Hit
AAAGGCAGATGCTTAGCTGACTGAGCCACCCAGGCGCCCTAAACAAAGCA8460.10104170896643647No Hit
CAGTAACTTTAAAAGCCATTGCCTGGCAAAAGAAAAGCAGACGATTTCTA8460.10104170896643647No Hit
TGGCAGAGGGACAAGAAGTAGACTCCTGGCTGAGTAGGGAGCCTGCCATG8450.10092227432226811No Hit
GAGCAAAAGCAGGGGGAGCAACTGGCAGAGGGACAAGAAGTAGACTCCTG8400.10032510110142628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)