FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_D08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_D08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490718
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA52220.3503009958959374No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA42140.2826825730956492No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC39330.26383259610469584No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT34580.23196875599543307No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT31580.21184422540010922No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC27430.1840052914099112No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA27110.18185867481307666No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG26880.18031579413410181No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT26200.17575423386582842No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC26010.1744796802614579No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG24250.1626732889788679No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG24020.16113040829989306No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC23610.15838005578519881No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC22570.15140355184548654No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG21570.1446953749803786No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA21140.14181085892838216No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT20130.1350356002946231No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA20030.13436478260811233No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA19660.1318827571680224No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA19540.13107777594420944No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA19370.1299373858771411No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA19020.1275895239743533No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA18820.1262478886013317No Hit
GAAGGAAAGAGAGCAAACAGGGGGAGCAACAGGCAGAGGGAGAGGGAGAA18620.1249062532283101No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA17610.11813099459455109No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA16990.11397192493818416No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA16980.11390484316953306No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA16800.11269737133381365No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC16490.11061783650563017No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA16460.11041659119967694No Hit
GTTTATTTATTTGACAGAGAGAGAAGGAAAGAGAGCAAACAGGGGGAGCA16380.10987993705046831No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG15640.10491588617028841No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG15120.10142763420043228No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC15090.10122638889447905No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)