FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953125
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATACAGGTGCCCCTGATAAGGTCTTTATAGATTTTGGATACTCGCCC86790.44436479999999995No Hit
CCCTTTATCTGATAATGTTATTCGCAAATATCTTCTCCCATTCTCTCCAT82280.42127359999999997No Hit
TTCTAGTACTGAACAAATCTTGACTTTCTTTGGAAAAGCTGAACAAAAGA74620.3820544No Hit
TCCTTACACCACCTTACACACAAAAACAGACTCAAAATGGATGAAAGACC72170.36951039999999996No Hit
ACCTTACACACAAAAACAGACTCAAAATGGATGAAAGACCTAAATGTGAG67310.34462719999999997No Hit
CTTCAATAAATGGTGTTGGAAAAACTGGACAGCCACATGCAGAAGAATGG65870.3372544No Hit
GTCTTTATAGATTTTGGATACTCGCCCTTTATCTGATAATGTTATTCGCA60430.3094016No Hit
CTACAGGGTCATTTGGTTGAGGCCTTTCTGCCACATTACCTAAACCAACC48630.2489856No Hit
CTTAATGACTAAGTCATACAGGTGCCCCTGATAAGGTCTTTATAGATTTT47220.2417664No Hit
GTCCTAGTGAGAGCGGCGCTCAGAAAGAGTCCGACAAGAGATCAAATGAG45730.2341376No Hit
ATGCAGAAGAATGGACTATTTCCTTACACCACCTTACACACAAAAACAGA45100.230912No Hit
AAGTCATACAGGTGCCCCTGATAAGGTCTTTATAGATTTTGGATACTCGC45080.2308096No Hit
CACAAAAACAGACTCAAAATGGATGAAAGACCTAAATGTGAGATAGGAAT44050.22553600000000001No Hit
CTCAATGACTGAGCCACCCTGGCACCCCGGCAATTACTTTTAACTCCAGG41310.2115072No Hit
ACCTAAATGTGAGATAGGAATCCATCAAAATCCTAGAGAACACACACAGC40470.2072064No Hit
GATAAGGTCTTTATAGATTTTGGATACTCGCCCTTTATCTGATAATGTTA40140.2055168No Hit
CTATACGCACAGTCTCAGCTCTCCTGGCTGGAAAAAGCTCAGAGCTCTCG39580.2026496No Hit
GAAAAAAGACAGTCTCTTCAATAAATGGTGTTGGAAAAACTGGACAGCCA37110.1900032No Hit
TCATAGGACCCTGGGATCATGACCTTAGCAGAAGGCAGATGCTTAATGAC37030.1895936No Hit
ACCTAAACCAACCCGTCAGAGGACCCTTACTTAATCTTCAGACACCCTGC36830.1885696No Hit
GTGTGTAGCAGCTCGAGAGGCACCGAGCAGCTGAGCCACGCGAAAACCTG35960.1841152No Hit
CACTAGGACTCTGGCCCTGACTTTTCTCTCTGCCCATGATAGCCAAGCGA35740.1829888No Hit
GGAAAAAAGACAGTCTCTTCAATAAATGGTGTTGGAAAAACTGGACAGCC34090.1745408No Hit
GCCACATGCAGAAGAATGGACTATTTCCTTACACCACCTTACACACAAAA33390.17095680000000002No Hit
ACCCTGTAGAGCGTAATTACCTATTAAAGGGATGGGAGGCACCAAATGGA32770.1677824No Hit
CAATAAATGGTGTTGGAAAAACTGGACAGCCACATGCAGAAGAATGGACT32750.16768No Hit
CTCTTCAATAAATGGTGTTGGAAAAACTGGACAGCCACATGCAGAAGAAT32130.1645056No Hit
AGAATGGACTATTTCCTTACACCACCTTACACACAAAAACAGACTCAAAA29530.15119359999999998No Hit
GATAATGTTATTCGCAAATATCTTCTCCCATTCTCTCCATTGTCTTTGGT29430.1506816No Hit
GACTATTTCCTTACACCACCTTACACACAAAAACAGACTCAAAATGGATG29110.14904320000000001No Hit
CCTTAGCAGAAGGCAGATGCTTAATGACTAAGTCATACAGGTGCCCCTGA27740.1420288No Hit
CCTTAAAGCCTGGAATACATCCGTCCCACCTAGAAACGGTAAGGAAGTCG27330.1399296No Hit
ATTAAAGGGATGGGAGGCACCAAATGGAGCAGATAAGAAAACCAGAAACA26910.13777920000000002No Hit
GGAAAGAATGTCCAAAGGAAAAAAGACAGTCTCTTCAATAAATGGTGTTG24880.12738560000000002No Hit
ATTTCCTTACACCACCTTACACACAAAAACAGACTCAAAATGGATGAAAG24560.1257472No Hit
GTCCAAAGGAAAAAAGACAGTCTCTTCAATAAATGGTGTTGGAAAAACTG24510.1254912No Hit
GGGAAGGACTGTGTGTAGCAGCTCGAGAGGCACCGAGCAGCTGAGCCACG24170.1237504No Hit
GTCTTTGGTGTTGTTGAGTGTTTCTTTTGCTGTGCAAAAGGAAGTCCCAG23780.12175359999999999No Hit
ATCCTAGAGAACACACACAGCAACCTCTTTGAACTTCTTCCTAGACACAT23410.1198592No Hit
GGTCTTTATAGATTTTGGATACTCGCCCTTTATCTGATAATGTTATTCGC23360.1196032No Hit
CCCTGGCACCCCGGCAATTACTTTTAACTCCAGGGTTTTTTGTTGTTGTT22860.11704319999999999No Hit
GTATTTATGCTGTAAGATGTCTGGACTGGAAAGTACTAATCGGAGGGAAC22720.1163264No Hit
GTCTAGGAAGAAGTTCAAAGAGGTTGCTGTGTGTGTTCTCTAGGATTTTG22590.1156608No Hit
TTCCTAGACACATCATCAAAAGCAAGGGAAGCAAGGGCAAAATGAACTAC22380.1145856No Hit
GGCAGAGAGAGGGGGGTAGGCAGGCTCCCTGCCAAGCAGAGAGCGGGGAC22030.1127936No Hit
CCTTACACCACCTTACACACAAAAACAGACTCAAAATGGATGAAAGACCT21970.1124864No Hit
ATCATGGGCAGAGAGAAAAGTCAGGGCCAGAGTCCTAGTGAGAGCGGCGC21800.11161599999999999No Hit
TTCTTTTGCTGTGCAAAAGGAAGTCCCAGTAGTTCATTTTGCCCTTGCTT21680.11100159999999999No Hit
AAATAATAAAGAGCTTTTCAAATCAAAAACACTGTAGCATAAATCCTGAA21380.10946560000000001No Hit
CTCCCATTCTCTCCATTGTCTTTGGTGTTGTTGAGTGTTTCTTTTGCTGT21310.1091072No Hit
CTCCCAATCATCTCCCAATTTCTAGTACTGAACAAATCTTGACTTTCTTT21200.108544No Hit
GCAGGAAAGAATGTCCAAAGGAAAAAAGACAGTCTCTTCAATAAATGGTG20820.10659840000000001No Hit
ATTTAAGGTCAAAGGGGCAACTCAAGGTCAAGTATCTGAATAACTAGCAG20690.1059328No Hit
CTCTTAGCTTATGGAAGCCAAGTCTTCCTGGATCATGCCAGAAGTTCCTG20360.10424320000000001No Hit
CCTTTGACCTCAGTGACCCAGCGCAGGTTTCATTAGCTGTTTCTGGTTTT20180.1033216No Hit
CCCTTTAATAGGTAATTACGCTCTACAGGGTCATTTGGTTGAGGCCTTTC20110.10296319999999999No Hit
GGTCAAAGGTGTGGGATAGAGGAAGTAGTTGTGGGACCAAAGAAGGGCCT20020.10250240000000001No Hit
GTACCGGATAGAATGATTGCTCGCTGAATATTTAGCTGGTGGAATGGAAA19990.10234879999999999No Hit
ATCATGACCTTAGCAGAAGGCAGATGCTTAATGACTAAGTCATACAGGTG19680.1007616No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)