FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_C02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_C02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920986
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA31230.16257276211279No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG27700.1441967822774346No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG24540.12774689664578504No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA23770.12373853843807295No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG21500.11192169021533734No Hit
TCCCCGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA20070.1044775964010149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)