FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848907
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG84580.45745946118436454No Hit
GTATAGAATTACCCATTGTCAAACAATGTATCAGACAACACAGGGTCTTG71620.38736399397049176No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT60620.32786938445254415No Hit
GTATAAATATTACACTTGGGAAAAGGAAAAAAAGTCACCAGCAGTTGAAC38280.20704124112245775No Hit
ATCCTAAAGGTGCCAAATTTATCTCCAGATAAATTTGGAAGCAACTGTGG37540.20303887648215946No Hit
ATATTACACTTGGGAAAAGGAAAAAAAGTCACCAGCAGTTGAACTATTCA36160.19557500728808966No Hit
GTGTGTGTGTTAAGCATGGAACACCACAAACCAGGTATTTTTTGCATGGT36030.19487188917560483No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA33730.18243210718548852No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC33080.17891651662306432No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA31240.16896469103097128No Hit
GTCTTGGCACTGTGTGTGTGTTAAGCATGGAACACCACAAACCAGGTATT29360.15879652140426748No Hit
TCCTAAAGGTGCCAAATTTATCTCCAGATAAATTTGGAAGCAACTGTGGA29160.1577148012312139No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG27350.14792523366507887No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA25020.13532319364900453No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA24700.1335924413721188No Hit
GTTCTATGCTGCTCTTCCATCAGTAAGGCATGAGATCAGCATCAGTAGCC24650.13332201132885538No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT24290.1313749150173589No Hit
CTCTAGGAATTCATAAAAGCATAAGAGGCAGCAAAATACAGAGGCAGATT24010.12986050677508387No Hit
TAATTACCCATTGTCATAAAATCTTGTCTTTAAATCAGTTTTGAGAACCG23340.12623674419535433No Hit
CCCATGGATTATCCTAAAGGTGCCAAATTTATCTCCAGATAAATTTGGAA23050.12466824994442664No Hit
GAATTATGAAGACTATTTGGGAGCTACCTCCTGTTGTCCAGGATGAGACA22840.12353244376272035No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT21330.11536545645616572No Hit
CTTTAGTTCTATGCTGCTCTTCCATCAGTAAGGCATGAGATCAGCATCAG21150.11439190830041748No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG20810.11255298400622638No Hit
TTGTACACCTGTGTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGG19710.10660352305443162No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA19690.10649535103712626No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA19200.10384513661314496No Hit
CTTATGTACCATGGAGTATGTTTCTGCTCGCTTAAGTAACCTCTACATCC19190.10379105060449227No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA19180.10373696459583959No Hit
GTATAGCCCAGTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGC19070.10314201850066011No Hit
GGATAATGGATCCTGCTACTTGGCATGCAAAGAAGGCAATAGGAAACGTT18850.10195212631030116No Hit
TATTTATACTTCTCCACAGTAAAATTAACTTGGGGAAATTTTTTCCTCTT18550.10032954605072078No Hit
GAATTACCCATTGTCAAACAATGTATCAGACAACACAGGGTCTTGGCACT18490.10000502999880469No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)