FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638159
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGAGATGGTTGATAATAGGACTTCATGACTCTATGTCTGCAAAG32340.19741673427304673No Hit
TTATAGCACATGTCCATGTAAGCTTAGACATAAAACTCAAGAGGTTTTTA25910.158165355133415No Hit
AAATAACACTGTACTCCTGGACCTAAAGAAGACAATGAAAAAAGGTATTT24550.14986335270263754No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT23770.14510191013204457No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG20630.12593405157863186No Hit
GGGTAAGGGTGTGAGGCCTGGGCTCTGGGCCAATTCTATCACCACTTAGT19760.1206232117883551No Hit
CCCTATATCCAGGGTAAGAGAAATTAAAGTACTACTGAGAAATCACTTTA18560.11329791552590437No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC18160.11085615010508747No Hit
ATACAGCGCTGAATACCTTTTCAGTTTTTCCCCAAAATAACACTGTACTC18090.11042884115644452No Hit
CTATTACTACCGTGGAAAACAGTTACCTTTAATCCGAGCAGTCAACAGCA17190.10493486895960648No Hit
ACATGATACAGCGCTGAATACCTTTTCAGTTTTTCCCCAAAATAACACTG16790.10249310353878957No Hit
GTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTAC16740.10218788286118746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)