FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S8_R1_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S8_R1_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756293
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA29860.17001718961471693No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG22730.1294203188192403No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG22500.12811074234196684No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG21080.12002553104749605No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA19720.1122819483992705No Hit
TCCCCGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA18590.10584794222831839No Hit
CTTTAGCGAGTCTCATCGGCTGCAAATCCATAAAACACCCTGCACTTCCA17820.10146370793483776No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)