FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S7_R1_G12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S7_R1_G12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11230
Sequences flagged as poor quality0
Sequence length151
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCT5985.325022261798754No Hit
AGCGAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGT5635.013357079252004No Hit
AAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAG3152.8049866429207477No Hit
CTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAA2852.5378450578806766No Hit
GCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTG2402.13713268032057No Hit
AAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCA2292.0391807658058774No Hit
GCGAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTG1681.495992876224399No Hit
AGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAG1591.4158504007123776No Hit
GATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGA1561.3891362422083704No Hit
GCGAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTG1511.344612644701692No Hit
GGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCG1241.1041852181656278No Hit
GCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGC1171.0418521816562778No Hit
AAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGGTT1131.006233303650935No Hit
TGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTT1080.9617097061442564No Hit
CGAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGC1070.9528049866429208No Hit
ACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGA1060.943900267141585No Hit
CTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCA1050.9349955476402494No Hit
GACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCT1000.8904719501335707No Hit
CACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGG960.8548530721282279No Hit
CCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCGC920.8192341941228851No Hit
GCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTG910.8103294746215495No Hit
CCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCAC880.7836153161175422No Hit
GGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGT840.7479964381121995No Hit
CCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGC830.7390917186108638No Hit
GAGCGAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCC770.6856634016028494No Hit
TCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACC770.6856634016028494No Hit
GAGCGAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCG760.6767586821015138No Hit
GCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGAC680.6055209260908282No Hit
GCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCG660.5877114870881568No Hit
GGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGC660.5877114870881568No Hit
GTTGAAGGATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACT610.5431878895814782No Hit
AAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGTTTACT550.4897595725734639No Hit
ACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGG540.4808548530721282No Hit
GCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAG540.4808548530721282No Hit
CTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTT520.4630454140694568No Hit
CGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCG460.40961709706144256No Hit
GGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCAC460.40961709706144256No Hit
TCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCG450.4007123775601068No Hit
GTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGTT440.3918076580587711No Hit
GCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTC440.3918076580587711No Hit
GTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGA440.3918076580587711No Hit
ACTTATTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTCTTATACA420.37399821905609976No Hit
TTATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGTTTACTATTTA420.37399821905609976No Hit
GCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCC420.37399821905609976No Hit
AAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGC410.365093499554764No Hit
GTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTATTGTATATC410.365093499554764No Hit
GCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGC390.3472840605520926No Hit
GGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTG390.3472840605520926No Hit
GCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGCACGCCGCTCACGG390.3472840605520926No Hit
AGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAA380.3383793410507569No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATAAGTCTGTCTCTTATACA370.3294746215494212No Hit
GCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCGCT360.32056990204808544No Hit
CTTTTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGC360.32056990204808544No Hit
GGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGC360.32056990204808544No Hit
CAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAAAATTAAAAATTAA350.3116651825467498No Hit
GTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAA350.3116651825467498No Hit
TTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGCACG330.2938557435440784No Hit
TTTGTGGGCCAGCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGCAC330.2938557435440784No Hit
CGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCC330.2938557435440784No Hit
AGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGT310.27604630454140694No Hit
CAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACC290.25823686553873554No Hit
CTATACAACATAGTAAACTATACAACAACACTTTTATAATATTTACTACT290.25823686553873554No Hit
CCTTCGTGCGCGATGCTTCGCACGAGCTGCGCACGCCGCTCACGGCCCTC290.25823686553873554No Hit
TTATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGCTGTCTCTTAT280.2493321460373998No Hit
CTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCG280.2493321460373998No Hit
CACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCG280.2493321460373998No Hit
CTATACAACATAGTAAACTATACAACAACACTTTTATAACTGTCTCTTAT280.2493321460373998No Hit
GTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGTTTACTATGTTGT270.2404274265360641No Hit
GATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACAAC260.2315227070347284No Hit
AAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCG250.22261798753339268No Hit
GTAGTAAATATTATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGT250.22261798753339268No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAAAATTAAAA240.21371326803205698No Hit
CGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCT240.21371326803205698No Hit
GGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGGTTGAAGCGCTGG240.21371326803205698No Hit
GAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGA240.21371326803205698No Hit
GCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGG230.20480854853072128No Hit
AGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATCTGTCTCT230.20480854853072128No Hit
GCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGC230.20480854853072128No Hit
ATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGCTGTCTCTTATAC230.20480854853072128No Hit
GCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCGCG230.20480854853072128No Hit
GCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCAGG220.19590382902938555No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATCTGTCTCTTATACACATC210.18699910952804988No Hit
GAAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCT210.18699910952804988No Hit
TGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAA210.18699910952804988No Hit
CGAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGC210.18699910952804988No Hit
ACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTC210.18699910952804988No Hit
ATGTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTATTGTATA210.18699910952804988No Hit
ACTTATTGTATATCCTTCAACACTTCAGTTGATACCTGCTTTCGCTCTGT200.17809439002671415No Hit
GAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTGTTGTATAGTTTAC200.17809439002671415No Hit
ATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACAACA200.17809439002671415No Hit
GCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAA200.17809439002671415No Hit
ACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGCGCGATGCT200.17809439002671415No Hit
GTTTACTATGTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTA200.17809439002671415No Hit
CAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCT190.16918967052537845No Hit
CAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGG190.16918967052537845No Hit
GCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGGCCCACA190.16918967052537845No Hit
AAGCATCGCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGG180.16028495102404272No Hit
CGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGC180.16028495102404272No Hit
GGCCAGCGCACCTTCGTGCGCGATGCTTCGCACGAGCTGCGCACGCCGCT180.16028495102404272No Hit
CAACTGAAGTGTTGAAGGATATACAATAAGTAGAAACTGTCTCTTATACA180.16028495102404272No Hit
GCTTCGCACGAGCTGCGCACGCCGCTCACGGCCCTCATCGGCGAGCTGGA170.15138023152270705No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAAACTGTCTC170.15138023152270705No Hit
TTTTTCTACTTATTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTC170.15138023152270705No Hit
AGGATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACA170.15138023152270705No Hit
GAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGA170.15138023152270705No Hit
CGCCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGT170.15138023152270705No Hit
AGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAACTGTCTCTT170.15138023152270705No Hit
TGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAG160.14247551202137132No Hit
AAACTATACAACATAGTAAACTATACAACAACACTTTTATAATATTTACT160.14247551202137132No Hit
CGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAAA160.14247551202137132No Hit
AGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAA150.13357079252003562No Hit
TGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTGG150.13357079252003562No Hit
TTCGTGCGCGATGCTTCGCACGAGCTGCGCACGCCGCTCACGGCCCTCAT150.13357079252003562No Hit
CAGCTCGCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCAT150.13357079252003562No Hit
CCTGCTCGACCGCCTCGAGGCCGCTTTTGTGGGCCAGCGCACCTTCGTGC150.13357079252003562No Hit
ATATTATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATAGTTTACTAT150.13357079252003562No Hit
TTACTATGTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTATT150.13357079252003562No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAATAAGTAGAAACTGTCTCTT150.13357079252003562No Hit
TTTCTACTTATTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTCTT150.13357079252003562No Hit
ATACAACATAGTAAACTATACAACAACACTTTTATAATATTTACTACTAA150.13357079252003562No Hit
GTATATCCTTCAACACTTCAGTTGATACCTGCTTTCGCTCTGTCTCTTAT140.1246660730186999No Hit
CCACAAAAGCGGCCTCGAGGCGGTCGAGCAGGCGGTTGAAGCGCTGGGTG140.1246660730186999No Hit
ATAGTAAACTATACAACAACACTTTTATAACTGTCTCTTATACACATCTC140.1246660730186999No Hit
TTATAAAAGTGTTGTTGTATAGTTTACTATGTTGTATCTGTCTCTTATAC140.1246660730186999No Hit
GTTGTATAGTTTACTATGTTGTATAGTTTACTATTTAATTTTTAATTTTT140.1246660730186999No Hit
ATAGTAAACTATACAACAACACTTTTATAATATTTACTACTAAACCTTGT140.1246660730186999No Hit
AGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAAGTCTGT130.1157613535173642No Hit
ATTGTATATCCTTCAACACTTCAGTTGATACCTGCTCTGTCTCTTATACA130.1157613535173642No Hit
GGTATCAACTGAAGTGTTGAAGGATATACAACTGTCTCTTATACACATCT130.1157613535173642No Hit
GTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCACGAAGGTGCGCTG130.1157613535173642No Hit
ATTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTCTTATACACATC130.1157613535173642No Hit
GCGCACGAAGGTGCGCTGGCCCACAAAAGCGGCCTCGAGGCGGTCGAGCA130.1157613535173642No Hit
CAACTGAAGTGTTGAAGGATATACAATAAGTCTGTCTCTTATACACATCT130.1157613535173642No Hit
AGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGA130.1157613535173642No Hit
GCCTCGAGGCGGTCGAGCAGGCGGTTGAAGCGCTGGGTGAGCAGGCCGAT130.1157613535173642No Hit
AAAGCAGCGACGAAATCGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTC130.1157613535173642No Hit
CTATACAACATAGTAAACTATACAACAACACTTTTATCTGTCTCTTATAC130.1157613535173642No Hit
CTGAAGTGTTGAAGGATATACAATAAGTAGAAAAAATTAAAAATTAAATA130.1157613535173642No Hit
TTGTATATCCTTCAACACTTCAGTTGATACCTGCTTTCCTGTCTCTTATA120.10685663401602849No Hit
CGGCCTGCTCACCCAGCGCTTCAACCGCCTGCTCGACCGCCTCGAGGCCG120.10685663401602849No Hit
AGCAGGTATCAACTGAAGTGTTGAAGGATATACAACTGTCTCTTATACAC120.10685663401602849No Hit
TTTTTCTACTTATTGTATATCCTTCAACACTTCAGTTGATACCTGCTTTT120.10685663401602849No Hit
GGATATACAATAAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACAA120.10685663401602849No Hit
TTTCTACTTATTGTATATCCTTCAACACTTCAGTTGATACCTGCTTTCGC120.10685663401602849No Hit
ATTGTATATCCTTCAACACTTCAGTTGATACCTGCTTTTGCCTGTCTCTT120.10685663401602849No Hit
AGCAAAAGCAGGGCCACTTCCAGCTCGCCGATGAGGGCCGTGAGCGGCGT120.10685663401602849No Hit
GCCGATGAGGGCCGTGAGCGGCGTGCGCAGCTCGTGCGAAGCATCGCGCA120.10685663401602849No Hit
AGCAGGTATCAACTGAAGTGTTGAAGGATATACAATCTGTCTCTTATACA120.10685663401602849No Hit
TTGTATATCCTTCAACACTTCAGTTGATACCCTGTCTCTTATACACATCT120.10685663401602849No Hit
GTATAGTTTACTATGTTGTATAGTTTACTATTTAATTTTTAATTTTTTCT120.10685663401602849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)