FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S7_R1_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S7_R1_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15207
Sequences flagged as poor quality0
Sequence length151
%GC61

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGC4332.847372920365621No Hit
GGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAG3662.406786348392188No Hit
AGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCC3642.3936345104228316No Hit
GAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGA3432.255540211744591No Hit
CCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATC3362.2095087788518444No Hit
GGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTG3232.124021832051029No Hit
AAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGAT3041.999079371342145No Hit
GTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGG2791.8346813967251925No Hit
CTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCC2691.7689222068784112No Hit
GTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCC2301.512461366475965No Hit
GATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATC2151.4138225817057934No Hit
GCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAA2151.4138225817057934No Hit
AGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCG2121.394094824751759No Hit
ATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATT2041.3414874728743342No Hit
CCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAATTCAACGGCATCG1921.2625764450581969No Hit
GGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATG1911.2560005260735188No Hit
GCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTT1781.1705135792727033No Hit
AGCGAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCG1771.1639376602880254No Hit
CTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTT1761.1573617413033472No Hit
AGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTT1721.1310580653646347No Hit
CCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTC1661.091602551456566No Hit
GTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGT1661.091602551456566No Hit
TCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCC1541.0126915236404288No Hit
GTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGC1410.9272045768396133No Hit
CTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGA1380.9074768198855789No Hit
AAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAG1370.9009009009009009No Hit
CTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTC1300.8548694680081542No Hit
GGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGG1200.7891102781613731No Hit
CCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCC1170.7693825212073387No Hit
CGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGC1150.7562306832379825No Hit
GTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTT1140.7496547642533045No Hit
AGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACT1140.7496547642533045No Hit
CCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCG1140.7496547642533045No Hit
CCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATG1130.7430788452686263No Hit
GAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGG1120.7365029262839482No Hit
CAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTG1100.7233510883145919No Hit
AGCGAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAG1090.7167751693299138No Hit
TTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCG1060.6970474123758795No Hit
GGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTG1060.6970474123758795No Hit
ACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTC950.6247123035444203No Hit
GGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGG880.5786808706516736No Hit
CTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGA870.5721049516669955No Hit
CAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGA860.5655290326823174No Hit
CTGCATGGGTTGGCGGACGGGAGTGCGTGAGAGCGTGGTAACGAGTCGAT840.5523771947129611No Hit
GAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACC840.5523771947129611No Hit
GGGAAAGTGGATCGGCGGACGCTGCATGGGTTGGCGGACGGGAGTGCGTG820.5392253567436049No Hit
CTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCA810.5326494377589268No Hit
CCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAAT790.5194975997895707No Hit
GCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGA780.5129216808048924No Hit
GCGAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGT770.5063457618202144No Hit
AAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGA770.5063457618202144No Hit
CGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGC760.49976984283553627No Hit
TTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTC720.47346616689682386No Hit
CGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTG720.47346616689682386No Hit
TGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTG720.47346616689682386No Hit
GAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTTGGCGGACG700.4603143289274676No Hit
AGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACG700.4603143289274676No Hit
GCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGC680.4471624909581114No Hit
AGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCA660.4340106529887552No Hit
GGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAA660.4340106529887552No Hit
TCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCAT650.4274347340040771No Hit
CCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCA650.4274347340040771No Hit
GTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAG620.4077069770500427No Hit
GGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGG580.3814033011113303No Hit
CTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCAT580.3814033011113303No Hit
CCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCC560.3682514631419741No Hit
CTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCT540.3550996251726179No Hit
AGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGG530.34852370618793976No Hit
ACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCG520.34194778720326163No Hit
GAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGA520.34194778720326163No Hit
ACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCG520.34194778720326163No Hit
AGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATA520.34194778720326163No Hit
GTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGT510.33537186821858356No Hit
AACCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAATTCAACGGCAT470.30906819227987115No Hit
GCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCG470.30906819227987115No Hit
CACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCC450.2959163543105149No Hit
GGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGG440.2893404353258368No Hit
CCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCAT440.2893404353258368No Hit
GGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGT440.2893404353258368No Hit
TCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCC430.2827645163411587No Hit
CCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAATTCAAC430.2827645163411587No Hit
CGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCT420.27618859735648055No Hit
TCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACC420.27618859735648055No Hit
GAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTAC410.26961267837180247No Hit
GAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGT400.26303675938712434No Hit
GTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTTGGCGG370.24330900243309003No Hit
GAGCGAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGC370.24330900243309003No Hit
AAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAG340.2235812454790557No Hit
TTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCAC330.2170053264943776No Hit
GCGTCCGCCGATCCACTTTCCCAGTCGAATTCAACGGCATCGCAGACACG330.2170053264943776No Hit
CGAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTC330.2170053264943776No Hit
GCGAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGC330.2170053264943776No Hit
GCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCAC330.2170053264943776No Hit
GCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGT310.20385348852502136No Hit
ACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGAT300.19727756954034328No Hit
GCATGGGTTGGCGGACGGGAGTGCGTGAGAGCGTGGTAACGAGTCGATGG290.19070165055566515No Hit
CTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTT290.19070165055566515No Hit
GCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACG290.19070165055566515No Hit
GTCGAATTCAACGGCATCGCAGACACGAGCGTGATCCGCTCCGGTACCTT280.18412573157098705No Hit
GCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTC280.18412573157098705No Hit
CGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGAT280.18412573157098705No Hit
GCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTT270.17754981258630895No Hit
CTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCA270.17754981258630895No Hit
ATGCAGCGTCCGCCGATCCACTTTCCCAGTCGAATTCAACGGCATCGCAG270.17754981258630895No Hit
CCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTTGGC260.17097389360163082No Hit
CGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGG260.17097389360163082No Hit
CGCTCTCACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCAC260.17097389360163082No Hit
ACGCACTCCCGTCCGCCAACCCATGCAGCGTCCGCCGATCCACTTTCCCA260.17097389360163082No Hit
AGCGTGATCCGCTCCGGTACCTTGTATGCCGCCAGCCTATCCGCCACAAA260.17097389360163082No Hit
GGCGCGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTC250.1643979746169527No Hit
GCAAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGC250.1643979746169527No Hit
GTGGATCGGCGGACGCTGCATGGGTTGGCGGACGGGAGTGCGTGAGAGCG240.1578220556322746No Hit
AGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGGTCC240.1578220556322746No Hit
TCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCC230.15124613664759648No Hit
CATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCA230.15124613664759648No Hit
AAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGTCAGT220.1446702176629184No Hit
GGCGGACGCTGCATGGGTTGGCGGACGGGAGTGCGTGAGAGCGTGGTAAC220.1446702176629184No Hit
ATGCCGTTGAATTCGACTGGGAAAGTGGATCGGCGGACGCTGCATGGGTT220.1446702176629184No Hit
CCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCC220.1446702176629184No Hit
GGTTGGCGGACGGGAGTGCGTGAGAGCGTGGTAACGAGTCGATGGGAGGA210.13809429867824027No Hit
CCGGGGGTAGAGGAGTTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATA210.13809429867824027No Hit
GAGCAAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGC200.13151837969356217No Hit
GCGTGAGAGCGTGGTAACGAGTCGATGGGAGGATGGAGGAAAGGGGAACG200.13151837969356217No Hit
GCAAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCGT190.12494246070888407No Hit
AGCAAAAGCAGGAAGGAAGTAACGCCGGCTCCGGGGGTAGAGGAGTTGCG190.12494246070888407No Hit
CACTTTCCCAGTCGAATTCAACGGCATCGCAGACACGAGCGTGATCCGCT180.11836654172420596No Hit
CGTTCCCCTTTCCTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGC180.11836654172420596No Hit
CACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCTTTCCTCCATCCT180.11836654172420596No Hit
GCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGG180.11836654172420596No Hit
CCACTTTCCCAGTCGAATTCAACGGCATCGCAGACACGAGCGTGATCCGC180.11836654172420596No Hit
CATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGCGATGCCGTTGAAT180.11836654172420596No Hit
CCGATCCACTTTCCCAGTCGAATTCAACGGCATCGCAGACACGAGCGTGA170.11179062273952785No Hit
CCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGAGGGCGCGTTCCCCT170.11179062273952785No Hit
GGAGTGCGTGAGAGCGTGGTAACGAGTCGATGGGAGGATGGAGGAAAGGG170.11179062273952785No Hit
GGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGT170.11179062273952785No Hit
GGATCACGCTCGTGTCTGCGATGCCGTTGAATTCGACTGGGAAAGTGGAT170.11179062273952785No Hit
GATAGGCTGGCGGCATACAAGGTACCGGAGCGGATCACGCTCGTGTCTGC170.11179062273952785No Hit
ATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGCCAACCCATGCAG160.10521470375484973No Hit
TCCCAGTCGAATTCAACGGCATCGCAGACACGAGCGTGATCCGCTCCGGT160.10521470375484973No Hit
GAGCGAAAGCAGGCCTAGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAA160.10521470375484973No Hit
TTGCGTCAGTTTGTGGCGGATAGGCTGGCGGCATACAAGGTACCGGAGCG160.10521470375484973No Hit
CTCCATCCTCCCATCGACTCGTTACCACGCTCTCACGCACTCCCGTCCGC160.10521470375484973No Hit
AGTCCGTACTCTCCTCACGCGCCCTCAAGCTGAAGCCGCGGGCGCGTTCC160.10521470375484973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)