FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S7_R1_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S7_R1_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296367
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA69600.303087442033438No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA65060.2833170830272339No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC59590.2594968487179967No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT42090.18328951774694552No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT40180.17497203190953362No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC38850.16918027475573372No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT37650.16395462920343307No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG36530.15907736002128578No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG36500.1589467188824783No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC36240.1578144956794798No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC35850.1561161608749821No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT32810.14287785880915377No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG32570.14183272969869365No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT32180.14013439489419593No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC31480.13708610165535387No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA31080.135344219804587No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA30870.13442973183293438No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC30810.13416844955531934No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT30450.13260075588962913No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT30390.13233947361201412No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA30220.13159917382543818No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT30210.13155562677916902No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA29560.1287250687716728No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT29440.12820250421644275No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA29140.12689609282836758No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG28560.12437036414475562No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT28520.12419617595967891No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT28420.12376070549698719No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC26470.11526903147449864No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA26410.11500774919688359No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT26030.11335296143865506No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC25730.11204655005057988No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT25440.1107836857087739No Hit
AACTTATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA25370.1104788563848897No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA25210.10978210364458293No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA24880.10834505111770025No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG24820.10808376884008522No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC24600.10712573382216345No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC24380.10616769880424166No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC24140.10512256969378152No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT23650.10298876442659209No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT23610.10281457624151541No Hit
GCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTCTCTTTTGAGCT23530.10246619987136203No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC23500.10233555873255451No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT23400.10190008826986278No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC23290.10142107076090189No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT23120.10068077097432596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)