FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S7_R1_A07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S7_R1_A07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650212
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTATTATGGATGAGAAAAAGAACTCTTGCTAATATTTAATTCCTTTTC87820.532174047940507No Hit
ATTTAATTCCTTTTCTCCTAAGAAAAGCTTATCTCTGAAAGGTTTTGCAA65750.3984336557969521No Hit
GTATTAGATTATCTGTTAAGTTCCCCCTGCAGATCTGGGCATTAGCCTCA52760.31971649703189653No Hit
AAATTGTATTGTGGGGTTACTGGTATATTGAACAACTCTTGTATTTGGAT50710.307293850729482No Hit
CATTTAATGAGATGTAAAAATTATAATGGCATTTTTCTTTTAGTAGCCAG42970.2603907861535366No Hit
GAAATAGATACCTTCCTACAGTAAATTGTATTGTGGGGTTACTGGTATAT40520.2455442088652852No Hit
TGCTAATATTTAATTCCTTTTCTCCTAAGAAAAGCTTATCTCTGAAAGGT35030.21227575608467275No Hit
GGTATATTGAACAACTCTTGTATTTGGATACTACCTTAGCAGCATGTGCC30280.18349157562785873No Hit
GTATGTTCTTGCTCCAAGCAGTGATAATGGATAAAATGGAAGGAATGGGT29840.18082525154343804No Hit
ATCTAATACAGTCCACCCCTGGTACTAGTGCTGACCCTCTTGATCCCACA29280.1774317481632663No Hit
CTGGTATATTGAACAACTCTTGTATTTGGATACTACCTTAGCAGCATGTG28970.17555320164924265No Hit
GAAAAAGAACTCTTGCTAATATTTAATTCCTTTTCTCCTAAGAAAAGCTT27470.166463460452354No Hit
ATATTGAACAACTCTTGTATTTGGATACTACCTTAGCAGCATGTGCCTGT26350.15967645369201047No Hit
GTATTGTGGGGTTACTGGTATATTGAACAACTCTTGTATTTGGATACTAC24400.14785979013605524No Hit
GTCATAGAGAGGTGCCCGTGGTTTCTGAGGGCCAGGCTGTGGGCCGCAGC24100.14604184189667752No Hit
CCTTCCTACAGTAAATTGTATTGTGGGGTTACTGGTATATTGAACAACTC23450.14210295404469245No Hit
ATATAATATTGGGTTCTCACATAGCAGGAGTGAATAATCTTTGTGAGAAT21970.13313440939709564No Hit
GTGATAATGGATAAAATGGAAGGAATGGGTGAGTCATTCAAACATACTCC21460.1300438973901535No Hit
ATTATATTCCTTATTATGGATGAGAAAAAGAACTCTTGCTAATATTTAAT21140.12810475260148393No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA20960.1270139836578573No Hit
ATACTGGATTGCTCCATAAGTGGAACTCTACCCTGAAACAGCTTTGGAAA19410.11762125108773903No Hit
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA19200.11634868732017462No Hit
ATATTATATGAGGCTAATGCCCAGATCTGCAGGGGGAACTTAACAGATAA19040.11537911492583983No Hit
ACCTTAGCAGCATGTGCCTGTTTTTGATAAGGTAATTTCTGATATGTGAC18510.11216740636960584No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT18150.10998586848235258No Hit
AAGAAAAGCTTATCTCTGAAAGGTTTTGCAAAGTATGTTCTTGCTCCAAG17930.10865270644014224No Hit
GTAAATTGTATTGTGGGGTTACTGGTATATTGAACAACTCTTGTATTTGG17860.10822851851762076No Hit
ACAGTAAATTGTATTGTGGGGTTACTGGTATATTGAACAACTCTTGTATT17220.10435022894028162No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG17070.10344125482059274No Hit
GATGTAAAAATTATAATGGCATTTTTCTTTTAGTAGCCAGGAGATTTGGC16890.1023504858769661No Hit
TTATGGATGAGAAAAAGAACTCTTGCTAATATTTAATTCCTTTTCTCCTA16580.10047193936294246No Hit
GTCAATAACTGAATGTTTACATAGTTGAGTCTGCAAGTCTTTTTCTTTGT16510.10004775144042098No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)