FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCA494.121110176619007No Hit
GTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCC423.532380151387721No Hit
AGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTC393.280067283431455No Hit
GGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGG393.280067283431455No Hit
GGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCA383.1959629941127No Hit
AGCGAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCG373.1118587047939443No Hit
GAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGT312.607232968881413No Hit
AAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGC292.4390243902439024No Hit
GGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTC211.7661900756938604No Hit
CTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACC191.59798149705635No Hit
ATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAA191.59798149705635No Hit
AAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCT181.5138772077375946No Hit
GTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCC161.3456686291000841No Hit
GGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCA161.3456686291000841No Hit
CCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCG151.2615643397813288No Hit
CACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCC151.2615643397813288No Hit
GTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGC151.2615643397813288No Hit
GCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCG131.0933557611438183No Hit
TTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCCC131.0933557611438183No Hit
AGCGAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCC131.0933557611438183No Hit
CCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGC131.0933557611438183No Hit
GTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGT121.0092514718250631No Hit
GGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGG121.0092514718250631No Hit
GCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGC110.9251471825063078No Hit
GATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGG110.9251471825063078No Hit
CACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGG110.9251471825063078No Hit
GCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCG110.9251471825063078No Hit
GGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAAC110.9251471825063078No Hit
AAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTG100.8410428931875525No Hit
GCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCA100.8410428931875525No Hit
AGCAAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCC100.8410428931875525No Hit
GCGAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGG90.7569386038687973No Hit
GGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCAT90.7569386038687973No Hit
GTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACG90.7569386038687973No Hit
GAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGC90.7569386038687973No Hit
GAGCAAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGC80.6728343145500421No Hit
GGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGC80.6728343145500421No Hit
GCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTG80.6728343145500421No Hit
CGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCCCGACTG80.6728343145500421No Hit
CCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCA70.5887300252312868No Hit
CTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGG70.5887300252312868No Hit
GCAAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCG70.5887300252312868No Hit
CGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCC70.5887300252312868No Hit
GAGCGAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGC70.5887300252312868No Hit
GTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGA60.5046257359125316No Hit
GAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGC60.5046257359125316No Hit
GCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATG60.5046257359125316No Hit
TCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCG60.5046257359125316No Hit
CAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACC60.5046257359125316No Hit
CTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCG60.5046257359125316No Hit
ACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACATGCCCGCCA60.5046257359125316No Hit
GCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGG60.5046257359125316No Hit
CAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCAC60.5046257359125316No Hit
TGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTC50.42052144659377627No Hit
TTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGT50.42052144659377627No Hit
GCAAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGG50.42052144659377627No Hit
TCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGAT50.42052144659377627No Hit
GAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACATGCCCGC50.42052144659377627No Hit
AGCAAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCG50.42052144659377627No Hit
GCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGACCTGTCCCA50.42052144659377627No Hit
CAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCG40.33641715727502103No Hit
ACCTCGGGTTCCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGG40.33641715727502103No Hit
TCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCC40.33641715727502103No Hit
CGGCACCACCGTGAAGCCGGGCTGGAGATAGCGCTGCAGGAAGGGCAGGT40.33641715727502103No Hit
CCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGC40.33641715727502103No Hit
GGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTT40.33641715727502103No Hit
GGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGC40.33641715727502103No Hit
GCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCC40.33641715727502103No Hit
GCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTC40.33641715727502103No Hit
CCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAGATAGCGCTGC40.33641715727502103No Hit
TGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGC40.33641715727502103No Hit
GCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACATGCC40.33641715727502103No Hit
CCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAGATAG40.33641715727502103No Hit
GCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCC40.33641715727502103No Hit
GAGCGAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCC40.33641715727502103No Hit
GCGAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCG30.2523128679562658No Hit
AGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCAC30.2523128679562658No Hit
AGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCG30.2523128679562658No Hit
CATCCGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCGG30.2523128679562658No Hit
GAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGACCTGTCCCACTACC30.2523128679562658No Hit
AAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCCCGGCTT30.2523128679562658No Hit
AGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTT30.2523128679562658No Hit
GGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCCAGGCGGATGGCGG30.2523128679562658No Hit
TGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACC30.2523128679562658No Hit
GTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCAT30.2523128679562658No Hit
CGAAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGT30.2523128679562658No Hit
GTGGCGGGCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGG30.2523128679562658No Hit
CTTCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAG30.2523128679562658No Hit
TCCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTC30.2523128679562658No Hit
CTCGGGTTCCGCAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCT30.2523128679562658No Hit
GTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTG20.16820857863751051No Hit
CCTGCAGCGCTATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCG20.16820857863751051No Hit
GGGTCAGGGCTTTTTCCAGGTTCACCGGGCTGCCGCTCTTGAGGTCGTTC20.16820857863751051No Hit
TGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACATGC20.16820857863751051No Hit
GGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCCCGACTGCGAGC20.16820857863751051No Hit
GGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGGCATGTTCCACGCC20.16820857863751051No Hit
CCTCGGACCTGTCCCACTACCTGCCCTGCTTTCGCTCTGTCTCTTATACA20.16820857863751051No Hit
ACCGTGAAGCCGGGCTGGAGATAGCGCTGCAGGAAGGGCAGGTGCACTTC20.16820857863751051No Hit
GAGCAAAAGCAGGAAGTGCACCTGCCCTTCCTGCAGCGCTATCTCCAGCC20.16820857863751051No Hit
CAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGGCGGG20.16820857863751051No Hit
CGCCACCGCTGTGGTGATCTCCTCGGACCTGTCCCACTACTGTCTCTTAT20.16820857863751051No Hit
TCCGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGAC20.16820857863751051No Hit
TCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAAC20.16820857863751051No Hit
AAAGCAGGGCAGGTAGTGGGACAGGTCCGAGGAGATCACCACAGCGGTGG20.16820857863751051No Hit
GCATGTTCCACGCCAGGCGGATGGCGGCGGCGGCGACCTCGGGTTCCGCA20.16820857863751051No Hit
CCATCCGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCG20.16820857863751051No Hit
CAACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAGATAGCGC20.16820857863751051No Hit
GCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGAACCCGAGG20.16820857863751051No Hit
GCTCGCAGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCC20.16820857863751051No Hit
AGTCGGGGTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGT20.16820857863751051No Hit
ATCTCCAGCCCGGCTTCACGGTGGTGCCGCTCGCAGTCGGGGTTGCGGCT20.16820857863751051No Hit
CGCCTGGCGTGGAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGACCT20.16820857863751051No Hit
GTTGCGGAACCCGAGGTCGCCGCCGCCGCCATCCGCCTGGCGTGGAACAT20.16820857863751051No Hit
GATGGCGGCGGCGGCGACCTCGGGTTCCGCAACCCCGACTGCGAGCGGCA20.16820857863751051No Hit
GAACATGCCCGCCACCGCTGTGGTGATCTCCTCGGACCTGTCTCTTATAC20.16820857863751051No Hit
ACCCCGACTGCGAGCGGCACCACCGTGAAGCCGGGCTGGAGATAGCGCTG20.16820857863751051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)