FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_F12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_F12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794
Sequences flagged as poor quality0
Sequence length151
%GC77

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGC1206.688963210702341No Hit
AAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGC995.518394648829431No Hit
AAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCG854.738015607580825No Hit
ACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCG744.124860646599777No Hit
AGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGG603.3444816053511706No Hit
AGCGAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCG512.842809364548495No Hit
GGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCAT452.508361204013378No Hit
GGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTT392.1739130434782608No Hit
CAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCC331.839464882943144No Hit
GCGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCC251.3935340022296545No Hit
CAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGC251.3935340022296545No Hit
AGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGG251.3935340022296545No Hit
GTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCC231.282051282051282No Hit
GTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGAC191.0590858416945375No Hit
TCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGC170.947603121516165No Hit
CGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCG140.7803790412486065No Hit
GGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCG140.7803790412486065No Hit
CCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCC140.7803790412486065No Hit
GGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTA130.7246376811594203No Hit
CTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGC130.7246376811594203No Hit
GGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACGGCGG130.7246376811594203No Hit
GTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCC120.6688963210702341No Hit
CCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGTA120.6688963210702341No Hit
ATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCT120.6688963210702341No Hit
GCGAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGT110.6131549609810479No Hit
GCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCT110.6131549609810479No Hit
GATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACGGCGGG100.5574136008918618No Hit
AAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCG90.5016722408026756No Hit
AAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCC90.5016722408026756No Hit
GCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGT90.5016722408026756No Hit
CGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCT90.5016722408026756No Hit
GAGCGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCG80.4459308807134894No Hit
TGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGC70.39018952062430323No Hit
GCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCG70.39018952062430323No Hit
CAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCG70.39018952062430323No Hit
ACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCC70.39018952062430323No Hit
GCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGT70.39018952062430323No Hit
GACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGT70.39018952062430323No Hit
CTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCAC70.39018952062430323No Hit
CGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGGGG70.39018952062430323No Hit
CGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGGGC70.39018952062430323No Hit
GTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTG70.39018952062430323No Hit
CCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCT70.39018952062430323No Hit
GCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGC70.39018952062430323No Hit
CCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAGCCTC70.39018952062430323No Hit
GGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGC60.33444816053511706No Hit
AGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCA60.33444816053511706No Hit
GGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGG60.33444816053511706No Hit
AAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCC60.33444816053511706No Hit
GAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCG60.33444816053511706No Hit
CGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGC60.33444816053511706No Hit
GCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGC60.33444816053511706No Hit
ACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTG60.33444816053511706No Hit
CCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAG60.33444816053511706No Hit
CCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCCGCC50.2787068004459309No Hit
CAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCCGCC50.2787068004459309No Hit
TCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGAC50.2787068004459309No Hit
CATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGTAACGC50.2787068004459309No Hit
GCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAG50.2787068004459309No Hit
GTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGT50.2787068004459309No Hit
GCCACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCG50.2787068004459309No Hit
GAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGG50.2787068004459309No Hit
GGAAAGCAGGGGGAGACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGCTT50.2787068004459309No Hit
GAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGC50.2787068004459309No Hit
GCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGC50.2787068004459309No Hit
GAGCGAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGC50.2787068004459309No Hit
ACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATAGCCG40.2229654403567447No Hit
CCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGCGCCCGTCGGCACGACG40.2229654403567447No Hit
GCAAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCC40.2229654403567447No Hit
CCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGC40.2229654403567447No Hit
GTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCGCGCC40.2229654403567447No Hit
CCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGC40.2229654403567447No Hit
CCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGG40.2229654403567447No Hit
AGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACGGCGGGAAAGCAGGGG40.2229654403567447No Hit
CCGCCACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGC40.2229654403567447No Hit
CAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCAC30.16722408026755853No Hit
AGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACGGCGGGAAAGC30.16722408026755853No Hit
CGGTAACGCCGCCGGCGGCCCAGGCGCCCGTCGGCACGACGGCCACGCTC30.16722408026755853No Hit
AGCGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTTGGCCGC30.16722408026755853No Hit
CGTCACCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGCGCCCGTCGGCA30.16722408026755853No Hit
CGAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTG30.16722408026755853No Hit
CAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCAACGC30.16722408026755853No Hit
ACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCAACGCCC30.16722408026755853No Hit
ACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCAACGCCG30.16722408026755853No Hit
CGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCG30.16722408026755853No Hit
CGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCG30.16722408026755853No Hit
ACGTCACCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGCGCCCGTCGGC30.16722408026755853No Hit
GCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGT30.16722408026755853No Hit
GCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGA30.16722408026755853No Hit
GGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCGGCGGCGGT30.16722408026755853No Hit
GCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGACG30.16722408026755853No Hit
CAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCCGCCATAGC30.16722408026755853No Hit
GGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCCGCCCT20.11148272017837235No Hit
GCGACGACGTCACCTCGCAGCCGGTAACGCCGCCGGCGGCCCAGGCGCCC20.11148272017837235No Hit
CCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATAGCCGCGCTCGCCAGCC20.11148272017837235No Hit
GGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCAA20.11148272017837235No Hit
ACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCCGCCATCGCCG20.11148272017837235No Hit
GAGACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGCTTTCCCGCCGTCGC20.11148272017837235No Hit
GGCGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGC20.11148272017837235No Hit
CACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGC20.11148272017837235No Hit
CTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGGTAACGCCGCC20.11148272017837235No Hit
CGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGCGC20.11148272017837235No Hit
AAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTTCCG20.11148272017837235No Hit
CTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCAG20.11148272017837235No Hit
GAGCAAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCG20.11148272017837235No Hit
AGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCA20.11148272017837235No Hit
TCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCCGC20.11148272017837235No Hit
GCACGCGACGGCGGGAAAGCAGGGGGAGACGCATCGGTTGTCCTGCTTCT20.11148272017837235No Hit
AGCAAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGC20.11148272017837235No Hit
GCTTTCCCGCCGTCGCGTGCCGGCCATCGCCGCGCTCGCCCGCCTCGCCT20.11148272017837235No Hit
GGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCCGCCAT20.11148272017837235No Hit
CGCCGCCACAGGCGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATG20.11148272017837235No Hit
GGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGCGCCCCCC20.11148272017837235No Hit
GTGGCGGCGACGACGTCACCTCGCAGCCGGTAACGCCGCCGGCGGCCCAG20.11148272017837235No Hit
GACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGCTTTCCCGCCGTCGCGT20.11148272017837235No Hit
CCGAAAGCAGGACAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGT20.11148272017837235No Hit
GCTTTCCCGCCGTCGCGTGCCGGCCCTCGCCGCGCTCGCCCGCCTCGCCT20.11148272017837235No Hit
GGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGCGGCCCCC20.11148272017837235No Hit
CAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCGGCCATAGC20.11148272017837235No Hit
GGACAGCTACGCCAAAGTCAACCGGGCATGGGCTTCGCATTTTGAACTGC20.11148272017837235No Hit
CGCCTGGGCCGCCGGCGGCGTTACCGGCTGCGAGGTGACGTCGTGGGGCG20.11148272017837235No Hit
CTCGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCC20.11148272017837235No Hit
CGCCTTCATCCTCGCCGCCTGTGGCGGCGACGACGTCACCTCGCAGCCGG20.11148272017837235No Hit
GGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCGCCCGCGGCGTT20.11148272017837235No Hit
CGGGAAAGCAGGGGGAGACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGC20.11148272017837235No Hit
CAACCGATGCGTCTCCCCCTGCTTTCCCGCCGTCGCGTGCCCGCCATCGC20.11148272017837235No Hit
GGGAGACGCATCGGTTGTCCTGCTTTCGCTCCCCCTGCTTTCCCGCCGTC20.11148272017837235No Hit
AAAGCAGGGCGAGCGTGGCCGTCGTGCCGCCGGGCGCCTGGGCCGCCGGC20.11148272017837235No Hit
GGGCGAGCGTGGCCGTCGTGCCGCCGGGCGCCTGGGCCGCCGGCGGCGTT20.11148272017837235No Hit
CGTGCCGGCCATCGCCGCGCTCGCCAGCCTCGCCTTCATCCTCGCCGCCT20.11148272017837235No Hit
CGGCGAGGATGAAGGCGAGGCTGGCGAGCGCGGCGATGGCCGGCACGCGA20.11148272017837235No Hit
AGCGAAAGCAGGGCGAGCGTGGCCGTCGTGCCGACGGGCGCCTGGGCCCC20.11148272017837235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)