FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_F04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_F04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625197
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC72000.44302321503177766No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG61870.38069230991689007No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA58330.35891033517782767No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA50750.3122698355953155No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT40730.2506157715033931No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA35260.2169583133613956No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT34250.21074368215053316No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG33780.20785172505240904No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC32360.19911432275594898No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT30490.1876080253655403No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC29780.18323932421731026No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC29650.18243942119016957No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA29180.17954746409204544No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC29130.17923980908160672No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA28390.17468651492711346No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA27590.1697640347600937No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG27320.16810269770372455No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG27250.16767198068911032No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT27070.16656442265153087No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT26520.16318021753670478No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA26060.1603497914406684No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC25930.1595498884135277No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC25530.15708864833001784No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC24850.15290454018805105No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA24850.15290454018805105No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG24050.14798206002103131No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT23680.14570541294378467No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA23430.144167137891591No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT22460.13819863068907953No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA22260.1369680106473246No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA22110.1360450456160084No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG21940.1349990185805167No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT21870.13456830156590247No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC21480.13216859248448035No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT21030.12939969739053173No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA20540.12638467828823213No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA20320.12503099624230168No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT20300.12490793423812621No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA20070.12349272119010803No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA19220.11826258601264955No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC19000.1169089039667191No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA18680.11493991189991122No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA18620.11457072588738472No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA18550.1141400088727705No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC18390.11315551283936655No Hit
GAAGGAAAGAGAGCAAACAGGGGGAGCAACAGGCAGAGGGAGAGGGAGAA18300.11260173382057682No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG18090.11130958277673414No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT17590.1082330326723468No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC17560.10804843966608355No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT17460.10743312964520609No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA17450.10737159864311835No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC17370.10687935062641636No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA17100.10521801357004719No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT16640.10238758747401085No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT16550.10183380845522114No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA16540.10177227745313339No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG16540.10177227745313339No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)